A novel host of <scp>MCR</scp>‐5 belonging to <i>Enterobacter</i> spp. isolated from hospital sewage water

Xu, Tingting; Ji, Yang; Song, Jingjie; Huang, Junxi; Chen, Rongchang; Qiu, Chen; Zhou, Kai

doi:10.1111/1758-2229.12937

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…For example, up to 31% of mcr -resistant strains were isolated directly from the feces of patients [ 49 ]. mcr-1 [ 50 ], mcr-1.1 [ 51 ], mcr-1.4 [ 51 ], mcr-5 [ 52 ], mcr-10 [ 53 ] were isolated from hospital wastewater. Additionally, mcr-1 [ 54 ], mcr-5.3 [ 55 ], and mcr-8 [ 56 ] were isolated from animal waste.…”

Section: Discussionmentioning

confidence: 99%

mcr-1-Mediated In Vitro Inhibition of Plasmid Transfer Is Reversed by the Intestinal Environment

Yang

Shu²,

Hou³

et al. 2022

Antibiotics

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Colistin is regarded as an antibiotic of last resort against multidrug-resistant Gram-negative bacteria, including Klebsiella pneumoniae and Escherichia coli. Colistin resistance is acquired by microorganisms via chromosome-mediated mutations or plasmid-mediated mobile colistin resistance (mcr) gene, in which the transfer of mcr is the predominant factor underlying the spread of colistin resistance. However, the factors that are responsible for the spread of the mcr gene are still unclear. In this study, we observed that mcr-1 inhibited the transfer of the pHNSHP45 backbone in liquid mating. Similar inhibitory effect of mcr-1.6 and chromosomal mutant ΔmgrB suggested that colistin resistance, acquired from either plasmid or chromosomal mutation, hindered the transfer of colistin resistance-related plasmid in vitro. Dual plasmid system further proved that co-existing plasmid transfer was reduced too. However, this inhibitory effect was reversed in vivo. Some factors in the gut, including bile salt and anaerobic conditions, could increase the transfer frequency of the mcr-1-containing plasmid. Our results demonstrated the potential risk for the spread of colistin resistance in the intestine, provide a scientific basis against the transmission of colistin resistance threat.

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Section: Discussionmentioning

confidence: 99%

mcr-1-Mediated In Vitro Inhibition of Plasmid Transfer Is Reversed by the Intestinal Environment

Yang

Shu²,

Hou³

et al. 2022

Antibiotics

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“…In addition, the mcr-5 gene has been detected in an Enterobacter sp. isolated from hospital sewage in China ( 13 ), and an MCR-5.3-producing Stenotrophomonas sp. has been isolated from animal waste in China ( 14 ).…”

Section: Observationmentioning

confidence: 99%

MCR-5-Producing Colistin-Resistant Cupriavidus gilardii Strain from Well Water in Batna, Algeria

Cherak

Loucif

Khedher

et al. 2021

mSphere

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“…Molecular mechanisms of colistin resistance can be attributed to chromosomal mutations and plasmid-mediated mobile colistin resistance genes ( mcr ). The first mcr gene, mcr-1 was identified from Escherichia coli in 2016 in China [5] , which variants have also been found in Enterobacter spp., including mcr-1 [6] , mcr-3 [7] , mcr-4 [8] , mcr-5 [9] , mcr-9 [10] , and mcr-10 [11] . The modification of LPS via two-component system (TCS) was a common strategy to reduce colistin susceptibility in Gram-negative bacteria [4] .…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology and genomic characterization of a plasmid-mediated mcr-10 and blaNDM-1 co-harboring multidrug-resistant Enterobacter asburiae

Jiang

et al. 2023

Computational and Structural Biotechnology Journal

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A novel host of MCR‐5 belonging to Enterobacter spp. isolated from hospital sewage water

Cited by 4 publications

References 17 publications

mcr-1-Mediated In Vitro Inhibition of Plasmid Transfer Is Reversed by the Intestinal Environment

mcr-1-Mediated In Vitro Inhibition of Plasmid Transfer Is Reversed by the Intestinal Environment

MCR-5-Producing Colistin-Resistant Cupriavidus gilardii Strain from Well Water in Batna, Algeria

Molecular epidemiology and genomic characterization of a plasmid-mediated mcr-10 and blaNDM-1 co-harboring multidrug-resistant Enterobacter asburiae

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