A novel homogeneous assay format utilising proximity dependent fluorescence energy transfer between particulate labels

Valanne, Antti; Lindroos, Hanne; Lövgren, Timo; Soukka, Tero

doi:10.1016/j.aca.2005.03.030

Cited by 25 publications

(24 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[33] An optical FRET-based multiion sensor has been designed by incorporating two independent sensing systems and QDs in submicron spheres. [34] Apart from some bioassays based on ruthenium-or europium-chelate-doped polymer NPs, [35,36] the use of fluorescent polymer NPs for the elaboration of FRET-based sensors or probes is still in its infancy. [1] We have recently shown that ligand-functionalized fluorescent polymer NPs are efficient and selective sensors for cupric ions in water using the quenching of fluorescence by energy transfer from dyes to metal complexes as a transduction signal.…”

Section: Introductionmentioning

confidence: 99%

A Cascade FRET‐Mediated Ratiometric Sensor for Cu²⁺Ions Based on Dual Fluorescent Ligand‐Coated Polymer Nanoparticles

Frigoli

Ouadahi

Larpent

2009

Chemistry A European J

View full text Add to dashboard Cite

Core-shell type dual fluorescent nanoparticles (NPs) in the 16 nm diameter range with a selective ligand (cyclam) attached to the surface and two fluorophores--9,10-diphenyl-anthracene (donor, D) and pyrromethene PM 567 (acceptor, A)--embedded within the polymer core were synthesized and their fluorescent and copper-sensing properties were studied and compared to single D-doped and A-doped NPs. The acceptor (A) and donor (D) dyes were chosen to allow two sequential Förster resonance energy transfer (FRET) processes from D to A and from the encapsulated dyes to copper complexes that form at the surface and act as quenchers. NPs with different D/A loads were readily obtained by two consecutive entrapments of the dyes. Dual NPs present tunable fluorescence emission that is dependent on the doping ratio. FRET from D to A results in sensitized emission from A upon excitation of D, with FRET efficiencies reaching 80 % at high acceptor loads. A 9-fold amplification of the signal of A is observed at high D-to-A ratios. Single- and dual-dye-doped NPs were used to detect the presence of cupric ions in water by using the quenching of fluorescence as a transduction signal. In accordance with the spectral overlaps and the values of the critical distance (R0) of D- and A-copper complex pairs, the acceptor is much more sensitive than the donor. In dual fluorescent NPs, the sensitized emission of A is efficiently attenuated whereas the remaining emission of D is much less affected, allowing the detection of copper in a ratiometric manner upon excitation at a single (D) wavelength. Dual-dye-doped NPs with the highest acceptor loads (23 A-per-NP) were found to be the most sensitive for the detection of copper over a wide range of concentrations (20 nM to 8.5 microM). Owing to its great convenience and modularity, the cascade FRET strategy based on dual fluorescent NPs holds great promise for the design of various sensing nanodevices.

show abstract

Section: Introductionmentioning

confidence: 99%

A Cascade FRET‐Mediated Ratiometric Sensor for Cu²⁺Ions Based on Dual Fluorescent Ligand‐Coated Polymer Nanoparticles

Frigoli

Ouadahi

Larpent

2009

Chemistry A European J

View full text Add to dashboard Cite

show abstract

“…The limit of detection was slightly better compared with assay using Tb chelates and FRET-based detection [42] and comparable to assay using Eu nanoparticles and FRET-based detection [43]. The obtained limit of detection also enables further development of the PSA assay for clinical applications where a cutoff of 4 ng/ ml is used, and patients with higher concentrations are examined further [44].…”

Section: Homogeneous Psa Assaymentioning

confidence: 74%

Precise construction of oligonucleotide–Fab fragment conjugate for homogeneous immunoassay using HaloTag technology

Päkkilä

Peltomaa

Lamminmäki

et al. 2015

Analytical Biochemistry

Self Cite

View full text Add to dashboard Cite

“…In the absence of inhibitor, the enzyme cleaves the substrate releasing the quencher, leading to emission of the fluorescent acceptor. Another FRET system has been described in a homogeneous sandwich immunoassay for prostate specific antigen (PSA) using Eu(III) chelate-doped donor NPs and near-infrared fluorescent acceptor microparticles, which were coated with two different PSA antibodies [56].…”

Section: Individual Assaysmentioning

confidence: 99%

High Throughput Bioassays Using Nanoparticles

Gómez‐Hens¹,

Fernández-Romero²,

Aguilar-Caballos³

2010

CCHTS

View full text Add to dashboard Cite

An overview of the usefulness of different nanoparticles to improve the features of high throughput separation and individual and multiplexed detection bioassays is presented. Although the development of microarray and microfluidic systems has expanded the capabilities of these high throughput assays, the combined use of NPs and these devices has provided them with new applications in drug discovery, proteomic and genomic studies, and clinical diagnosis. This article reviews the wide application field of magnetic, gold, silver, semiconductor and other nanoparticles in high throughput bioassays. Also, the versatility of the detection systems described shows that NPs are useful alternatives to fluorescent dyes, which are often used in these assays.

show abstract

A novel homogeneous assay format utilising proximity dependent fluorescence energy transfer between particulate labels

Cited by 25 publications

References 12 publications

A Cascade FRET‐Mediated Ratiometric Sensor for Cu²⁺Ions Based on Dual Fluorescent Ligand‐Coated Polymer Nanoparticles

A Cascade FRET‐Mediated Ratiometric Sensor for Cu²⁺Ions Based on Dual Fluorescent Ligand‐Coated Polymer Nanoparticles

Precise construction of oligonucleotide–Fab fragment conjugate for homogeneous immunoassay using HaloTag technology

High Throughput Bioassays Using Nanoparticles

Contact Info

Product

Resources

About

A novel homogeneous assay format utilising proximity dependent fluorescence energy transfer between particulate labels

Cited by 25 publications

References 12 publications

A Cascade FRET‐Mediated Ratiometric Sensor for Cu2+Ions Based on Dual Fluorescent Ligand‐Coated Polymer Nanoparticles

A Cascade FRET‐Mediated Ratiometric Sensor for Cu2+Ions Based on Dual Fluorescent Ligand‐Coated Polymer Nanoparticles

Precise construction of oligonucleotide–Fab fragment conjugate for homogeneous immunoassay using HaloTag technology

High Throughput Bioassays Using Nanoparticles

Contact Info

Product

Resources

About

A Cascade FRET‐Mediated Ratiometric Sensor for Cu²⁺Ions Based on Dual Fluorescent Ligand‐Coated Polymer Nanoparticles

A Cascade FRET‐Mediated Ratiometric Sensor for Cu²⁺Ions Based on Dual Fluorescent Ligand‐Coated Polymer Nanoparticles