A novel high-throughput single-molecule technique: DNA curtain

Cha, Jongjin; Lee, Ja Yil

doi:10.1007/s40042-020-00031-9

Search citation statements

Order By: Relevance

Paper Sections

Select...

Single-molecule Methods In Transcription Studies1

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2021

2023

Publication Types

Select...

Book3

Article1

Relationship

Self Cite0

Independent4

Authors

Journals

Cited by 4 publications

(1 citation statement)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The DNA curtain assay is performed by aligning DNA molecules on a surface ( 58 , 99 ) ( Fig. 3 A , left).…”

Section: Single-molecule Methods In Transcription Studiesmentioning

confidence: 99%

Probing steps in DNA transcription using single-molecule methods

Lee

Myong

2021

Journal of Biological Chemistry

View full text Add to dashboard Cite

Transcriptional regulation is one of the key steps in determining gene expression. Diverse single-molecule techniques have been applied to characterize the stepwise progression of transcription, yielding complementary results. These techniques include, but are not limited to, fluorescence-based microscopy with single or multiple colors, force measuring and manipulating microscopy using magnetic field or light, and atomic force microscopy. Here, we summarize and evaluate these current methodologies in studying and resolving individual steps in the transcription reaction, which encompasses RNA polymerase binding, initiation, elongation, mRNA production, and termination. We also describe the advantages and disadvantages of each method for studying transcription.

show abstract

“…The DNA curtain assay is performed by aligning DNA molecules on a surface ( 58 , 99 ) ( Fig. 3 A , left).…”

Section: Single-molecule Methods In Transcription Studiesmentioning

confidence: 99%