A Novel High-Molecular-Mass Bacteriocin Produced by Enterococcus faecium: Biochemical Features and Mode of Action

Vasilchenko, Alexey S.; Vasilchenko, Anastasia V.; Valyshev, A V; Рогожин, Е. А.

doi:10.1007/s12602-018-9392-0

Cited by 13 publications

(15 citation statements)

References 35 publications

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“…Earlier we successfully performed this approach for investigation of mode of action of the various antimicrobial peptides [ 25 ]. We showed that this effect is really occurred when PI was able to influx into the cells via a disordered barrier following displacement of the SYTO9 from the DNA [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina

et al. 2018

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Soil fungi are known to contain a rich variety of defense metabolites that allow them to compete with other organisms (fungi, bacteria, nematodes, and insects) and help them occupy more preferential areas at the expense of effective antagonism. These compounds possess antibiotic activity towards a wide range of other microbes, particularly fungi that belong to different taxonomical units. These compounds include peptaibols, which are non-ribosomal synthesized polypeptides containing non-standard amino acid residues (alpha-aminoisobutyric acid mandatory) and some posttranslational modifications. We isolated a novel antibiotic peptide from the culture medium of Emericellopsis alkalina, an alkalophilic strain. This peptide, called emericellipsin A, exhibited a strong antifungal effect against the yeast Candida albicans, the mold fungus Aspergillus niger, and human pathogen clinical isolates. It also exhibited antimicrobial activity against some Gram-positive and Gram-negative bacteria. Additionally, emericellipsin A showed a significant cytotoxic effect and was highly active against Hep G2 and HeLa tumor cell lines. We used NMR spectroscopy to reveal that this peptaibol is nine amino acid residues long and contains non-standard amino acids. The mode of molecular action of emericellipsin A is most likely associated with its effects on the membranes of cells. Emericellipsin A is rather short peptaibol and could be useful for the development of antifungal, antibacterial, or anti-tumor remedies.

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Section: Resultsmentioning

confidence: 99%

A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina

et al. 2018

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“…They are presumed to modulate the commensal intestinal flora. The mechanisms of action displayed by these phylogenetically diverse microorganisms range from immunomodulation [ 2 , 3 ] and epithelial barrier maintenance [ 4 ] to direct antagonism with pathogens via bacteriocin production [ 5 , 6 ] and competitive exclusion [ 7 , 8 ]. Short chain fatty acids, such as formic, acetic, propionic, butyric and lactic acids, are produced by probiotics during carbohydrate catabolism and play an important role in the decrease of pH that inhibits the growth of enteric pathogens [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

A Sensitive and Rapid Method to Determine the Adhesion Capacity of Probiotics and Pathogenic Microorganisms to Human Gastrointestinal Mucins

et al. 2018

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Mucus is the habitat for the microorganisms, bacteria and yeast that form the commensal flora. Mucins, the main macromolecules of mucus, and more specifically, the glycans that cover them, play essential roles in microbial gastrointestinal colonization. Probiotics and pathogens must also colonize mucus to have lasting positive or deleterious effects. The question of which mucin-harboured glycan motifs favour the adhesion of specific microorganisms remains very poorly studied. In the current study, a simple test based on the detection of fluorescent-labeled microorganisms raised against microgram amounts of mucins spotted on nitrocellulose was developed. The adhesion of various probiotic, commensal and pathogenic microorganisms was evaluated on a panel of human purified gastrointestinal mucins and compared with that of commercially available pig gastric mucins (PGM) and of mucins secreted by the colonic cancer cell line HT29-MTX. The latter two proved to be very poor indicators of adhesion capacity on intestinal mucins. Our results show that the nature of the sialylated cores of O-glycans, determined by MALDI MS-MS analysis, potentially enables sialic acid residues to modulate the adhesion of microorganisms either positively or negatively. Other identified factors affecting the adhesion propensity were O-glycan core types and the presence of blood group motifs. This test should help to select probiotics with enhanced adhesion capabilities as well as deciphering the role of specific mucin glycotopes on microbial adhesion.

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“…Bacteriocins are divided into four classes. Division by classes is carried out according to the https://www.id-press.eu/mjms/index methods of synthesis, physicochemical and amino acid composition, and antibacterial properties [9], [10].…”

Section: Bacteriocin Classificationmentioning

confidence: 99%

Screening and Characterization of the Antagonistic Properties of Microorganisms Isolated From Natural Sources

Le¹,

Asyakina²,

Velichkovitch³

et al. 2020

Open Access Maced J Med Sci

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BACKGROUND: Human infectious diseases caused by antibiotic-resistant bacterial pathogens present a serious problem for clinical medicine. Causative agents of nosocomial infections, such as Escherichia coli, Klebsiella pneumoniae, Enterobacter spp., are the most common among them. An active search for antimicrobial agents that can effectively combat drugresistant pathogens is underway. Antimicrobial substances of bacterial origin are of particular interest. Promising sources of microorganisms with antibiotic properties are natural sources: Soil, water, plants, etc. AIM: The purpose of this work is to screen and characterize the antagonistic properties of microorganisms isolated from natural sources in connection with the creation of new pharmaceutical substances. METHODS: The material for the isolation of microorganisms was the soil, water bodies, and plant objects of various municipal districts of the Kemerovo Region. Identification of the isolated microorganisms was carried out using the methods proposed in the directory “Bergey’s Manual of Determinative Bacteriology” and in the monograph Nesterenko et al. The selection of strains from soil samples was carried out according to standard methods described in “Methods of soil microbiological control. Methodical recommendations,” cultural-morphological properties of isolates were studied using conventional microbiological methods. RESULTS: The following results are obtained: (1) Lactic acid bacteria and other microorganisms antagonists from natural sources were isolated: Soil, water bodies, and plant objects; 20 isolates were isolated, their cultural and morphological properties were studied; isolated microorganisms were found to belong presumably to the genera Bacillus, Leuconostoc, Pedio-coccus, Lactobacillus, and Bacteroides; (2) Antimicrobial properties of lactic acid bacteria and other antagonistic microorganisms isolated from natural sources on solid and liquid nutrient media were studied; (3) 12 strains of 20 isolates with maximum antimicrobial properties were selected for further studies. CONCLUSION: Further research on the biochemical properties of lactic acid bacteria and other antagonist microorganisms isolated from natural sources, the study of antibiotic resistance of lactic acid bacteria and other antagonist microorganisms isolated from natural sources, as well as other more detailed studies will be conducted with selected 12 strains with maximum antimicrobial properties.

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A Novel High-Molecular-Mass Bacteriocin Produced by Enterococcus faecium: Biochemical Features and Mode of Action

Cited by 13 publications

References 35 publications

A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina

A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina

A Sensitive and Rapid Method to Determine the Adhesion Capacity of Probiotics and Pathogenic Microorganisms to Human Gastrointestinal Mucins

Screening and Characterization of the Antagonistic Properties of Microorganisms Isolated From Natural Sources

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