A Novel High-Mannose Specific Lectin from the Green Alga Halimeda renschii Exhibits a Potent Anti-Influenza Virus Activity through High-Affinity Binding to the Viral Hemagglutinin

Mu, Jinmin; Hirayama, Makoto; Sato, Yoji; Morimoto, Kanehisa; Hori, Kazuaki

doi:10.3390/md15080255

Cited by 42 publications

(53 citation statements)

References 39 publications

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“…Based on the qualitative HI assay, both of the P. australis and P. minor extracts did not have an affinity to the various simple sugars tested. This result is in line with previous reports that macroalgae lectins generally do not have an affinity to simple sugars but have more affinity to glycoconjugates (Hung et al, 2015;Mu, Hirayama, Sato, Morimoto, & Hori, 2017;Nagano et al, 2002;Singh & Walia, 2018). Lectincontaining extract of P. australis bond 7 glycoproteins, i.e., thyroglobulin from bovine thyroid gland (BTG), thyroglobulin from porcine thyroid gland (PTG), Asialo-Fetuin (aFe), Asialo-BSM (aBSM), Asialo-PTG (aPTG), Asialo-Tf (aTf), and Asialo-BTG (aBTG), while P. minor extract only bound 3 asialo glycoproteins, i.e.…”

Section: Resultssupporting

confidence: 93%

Extraction and Partial Characterization of Lectin from Indonesian Brown Algae Padina australis and Padina minor

Fajarningsih

Intaqta

Praseptiangga

et al. 2019

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Extraction and partial characterization of lectin from Indonesian Padina australis and Padina minor had been carried out. The crude extract of the P. australis and P. minor were examined for hemagglutination activity (HA) using native and trypsin-treated of rabbit and human A, B, O type erythrocytes. Both extracts agglutinated all of the trypsintreated erythrocytes tested in the HA assay. Strong HA was detected in the crude extract of P. minor with trypsintreated of human type A and O erythrocytes. However, the sugar-binding specificity study through the quantitative hemagglutination inhibition (HI) assay showed that P. minor extract could not specifically recognize the glycans tested. Apparently, the HA of the P. minor was more due to its co-extracted polyphenols content than its lectin content. On the other hand, the HI assay showed that asialo transferrin human (aTf) and asialo porcine thyroglobulin (aPTG) were the most powerful in inhibiting the HA of P. australis. Those indicated that P. australis protein extract was able to specifically recognized aTf and aPTG. The stability of P. australis and P. minor HA over various temperatures, pH ranges, and divalent cations studies showed that the P. minor HA was stable on a wide range of pH and temperature; not affected by the presence of EDTA, but decreased by Ca 2+ and Mg 2+ additions showed that P. minor protein extract was not a metallic protein. The HA of P. australis decreased at 60 o C and was inactivated at 90 o C; increased at strong acidic (pH 3 & 4) and strong basic (pH 9 & 10) and dependent by the presence of either EDTA or Ca 2+ and Mg 2+ divalent cation.

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Section: Resultssupporting

confidence: 93%

Extraction and Partial Characterization of Lectin from Indonesian Brown Algae Padina australis and Padina minor

Fajarningsih

Intaqta

Praseptiangga

et al. 2019

Squalen Bull. Marine Fisheries Postharvest Biotech.

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“…They are a structurally diverse group of highly specific and reversibly carbohydrate-binding proteins [70]. The three groups of macroalgae (Rhodophyta, Phaeophyta, and Chlorophyta) can produce lectins [71], and these lectins present great potential for the development of new drugs [72][73][74][75][76]. In fact, because of the highly specific way lectins bind to sugars outside cell surfaces inhibiting cell proliferation [77,78], lectins primarily show antiviral, antibacterial, and antifungal activities [73,[79][80][81].…”

Section: Griffithsinmentioning

confidence: 99%

Seaweed Secondary Metabolites with Beneficial Health Effects: An Overview of Successes in In Vivo Studies and Clinical Trials

et al. 2019

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Macroalgae are increasingly viewed as a source of secondary metabolites with great potential for the development of new drugs. In this development, in vitro studies are only the first step in a long process, while in vivo studies and clinical trials are the most revealing stages of the true potential and limitations that a given metabolite may have as a new drug. This literature review aims to give a critical overview of the secondary metabolites that reveal the most interesting results in these two steps. Phlorotannins show great pharmaceutical potential in in vivo models and, among the several examples, the anti-dyslipidemia activity of dieckol must be highlighted because it was more effective than lovastatin in an in vivo model. The IRLIIVLMPILMA tridecapeptide that exhibits an in vivo level of activity similar to the hypotensive clinical drug captopril should still be stressed, as well as griffithsin which showed such stunning results over a variety of animal models and which will probably move onto clinical trials soon. Regarding clinical trials, studies with pure algal metabolites are scarce, limited to those carried out with kahalalide F and fucoxanthin. The majority of clinical trials currently aim to ascertain the effect of algae consumption, as extracts or fractions, on obesity and diabetes.Studies focusing on the preparation of macroalgae extracts and their chemical characterization revealed a large range of seaweed compounds with very interesting biological activities including antitumor, anti-inflammatory, antimicrobial, antidiabetic, antivirus, antihypertensive, fat-lowering, and neuroprotective activities [12][13][14][15].The large volume of studies proving the seaweed compound activities in in vitro systems [16][17][18][19] hints the need for further advancements in the knowledge about macroalgae compound efficiency in living systems (in vivo) and their use in the development of pharmaceuticals. In vitro studies are very relevant and yield very important information, but they only represent the first step of a long process, and the results obtained rarely reveal anything about the effects of a compound in vivo, because the responses observed in vitro can be magnified, diminished, or totally different in a more complex and integrated system. In fact, in vivo studies and clinical trials are those which contribute most to truly understanding the real potential of compounds as future pharmaceuticals.In this regard, the present work intends to present insight into the results obtained in the last few years regarding secondary metabolites, such as phlorotannins, halogenated compounds, fucoxanthin, and fucosterol isolated from macroalgae, involved in in vivo studies and clinical trials, identifying the research opportunities and knowledge gaps, to valorize these compounds and their natural resources. The intention is not to present an exhaustive survey of all published works, but rather a selection of authors based on the following criteria: in-depth studies involving pure compounds most characteristic f...

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“…Mannose-specific Lectin from Green alga Halimeda renschii showed strong activity against influenza virus due to high affinity binding to hemagglutinins on envelopes of viruses [115].…”

Section: Glycoproteinsmentioning

confidence: 99%

Antimicrobial lead compounds from marine plants

Kurhekar¹

2020

Phytochemicals as Lead Compounds for New Drug Discovery

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A Novel High-Mannose Specific Lectin from the Green Alga Halimeda renschii Exhibits a Potent Anti-Influenza Virus Activity through High-Affinity Binding to the Viral Hemagglutinin

Cited by 42 publications

References 39 publications

Extraction and Partial Characterization of Lectin from Indonesian Brown Algae Padina australis and Padina minor

Extraction and Partial Characterization of Lectin from Indonesian Brown Algae Padina australis and Padina minor

Seaweed Secondary Metabolites with Beneficial Health Effects: An Overview of Successes in In Vivo Studies and Clinical Trials

Antimicrobial lead compounds from marine plants

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