A novel hierarchically structured siliceous packing to boost the performance of rotating bed enzymatic reactors

Szymańska, Katarzyna; Odrozek, K.; Zniszczoł, Aurelia; Pudło, Wojciech; Jarzębski, Andrzej B.

doi:10.1016/j.cej.2016.12.131

Cited by 21 publications

(26 citation statements)

References 25 publications

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“…tank vs. tube/column and the mode of operation: periodic/batch vs. continuous flow. Conventional stirred tank reactors (STR) [259,266,270,271] are still frequently applied, but basket and in particular rotating bed reactors [272] are increasingly used in batch operations. In continuous flow applications, packed-bed (micro)reactors [273] have been most popular, but structural (micro)reactors [274,275], lab-on-a-chip [276], and capillary microreactors are on the rise [277].…”

Section: Reactor Engineering For (Non)-leloir Glycosyltransferasesmentioning

confidence: 99%

“…The STRs are predominantly used in biotechnology owing to cost efficiency and versatility. However, vigorous mixing results in frequent collisions of fine biocatalyst particles resulting in tensile and shearing forces which enhance abrasion or disintegration of the enzyme or its carrier [272]. Moreover, the mixing on a medium and large scale can be insufficient to prevent “hot spot” formation, resulting in enzyme denaturation.…”

Section: Reactor Engineering For (Non)-leloir Glycosyltransferasesmentioning

confidence: 99%

See 1 more Smart Citation

Leloir Glycosyltransferases in Applied Biocatalysis: A Multidisciplinary Approach

Mestrom

Przypis

Kowalczykiewicz

et al. 2019

IJMS

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Enzymes are nature's catalyst of choice for the highly selective and efficient coupling of carbohydrates. Enzymatic sugar coupling is a competitive technology for industrial glycosylation reactions, since chemical synthetic routes require extensive use of laborious protection group manipulations and often lack regio-and stereoselectivity. The application of Leloir glycosyltransferases has received considerable attention in recent years and offers excellent control over the reactivity and selectivity of glycosylation reactions with unprotected carbohydrates, paving the way for previously inaccessible synthetic routes. The development of nucleotide recycling cascades has allowed for the efficient production and reuse of nucleotide sugar donors in robust one-pot multi-enzyme glycosylation cascades. In this way, large glycans and glycoconjugates with complex stereochemistry can be constructed. With recent advances, LeLoir glycosyltransferases are close to being applied industrially in multi-enzyme, programmable cascade glycosylations.hydroxynitrile lyases catalyzed the enzymatic hydrolysis of the glycoside amygdalin [3]. Moving almost two centuries forward, the largest volumetric biocatalytic industrial process is the application of glucose isomerase for the production of high fructose syrup for food and drink applications, producing fructose from glucose at 10 7 tons per year [4]. The secret of the success of enzymes in the production or treatment of carbohydrates and glycosides is their exquisite stereo-and regioselectivity. The excellent selectivity of enzymes is required due to the diversity of structural features of carbohydrates [5], comprising d-and l-epimers, ring size, anomeric configuration, linkages, branching, and oxidation state(s). Since drug targets often exhibit specificity for all of these structural features, the production process should not contain any side-products to prevent undesired side-effects [6].The challenge in the synthesis of carbohydrates is their wide variety of functionalities and stereochemistry ( Figure 1). (Poly)hydroxyaldehydes containing a terminal aldehyde are referred to as aldoses and (poly)hydroxyketones are defined as ketoses. In aqueous solutions, monosaccharides form equilibrium mixtures of linear open-chain and ring-closed 5-or 6-membered furanoses or pyranoses, respectively. For aldoses, the asymmetric ring forms at C-1. For ketoses, it closes at C-2 as an axial (α) or equatorial (β) hemiacetal or hemiketal, respectively (commonly defined as the anomeric center). A glycosidic linkage is a covalent O-, S-, N-, or C-bond connecting a monosaccharide to another residue resulting in a glycoside, while glucoside is specific for a glucose moiety. The equatorial or axial position of the glycosidic bond is referred to as α-(axial) or β-linkage (equatorial). The number of carbohydrates linked via glycosidic bonds can be subdivided into oligosaccharides with two to ten linked carbohydrates, while polysaccharides (glycans) contain more than ten glycosidic bonds. A glycan either con...

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Section: Reactor Engineering For (Non)-leloir Glycosyltransferasesmentioning

confidence: 99%

Section: Reactor Engineering For (Non)-leloir Glycosyltransferasesmentioning

confidence: 99%

Leloir Glycosyltransferases in Applied Biocatalysis: A Multidisciplinary Approach

Mestrom

Przypis

Kowalczykiewicz

et al. 2019

IJMS

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“…This results in very intensive homogenization of reactants even in the laminar flow rate range, which reduced diffusional times and enhanced mass transfer, while simultaneously providing ample surface for enzyme immobilization. 32,33 To visualize this: several aspects change when switching from a dissolved enzyme or an enzyme attached to a small particle to an enzyme immobilized on a monolith reactor surface. Both, the enzyme in solution or the enzyme on a particle will move together with the reaction mixture and thus move with a similar motion as the reaction mixture.…”

Section: Introductionmentioning

confidence: 99%

Hydroxynitrile lyases covalently immobilized in continuous flow microreactors

Helm

Bracco

Busch

et al. 2019

Catal. Sci. Technol.

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“…The permeability coefficient was three orders of magnitude lower in M3 than in M1, in perfect agreement with the smaller size of macropores in that material. The permeability of the monoliths and the packed bed reactor (Amberlyst) was calculated using Darcy equation [25], and the results obtained are shown in Table 2. The permeability coefficient was three orders of magnitude lower in M3 than in M1, in perfect agreement with the smaller size of macropores in that material.…”

Section: Introductionmentioning

confidence: 99%

Continuous-Flow Monolithic Silica Microreactors with Arenesulphonic Acid Groups: Structure–Catalytic Activity Relationships

et al. 2017

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Abstract:The performance of monolithic silica microreactors activated with sulphonic acid groups and a packed bed reactor with Amberlyst 15 resin were compared in the esterification of acetic acid with n-butanol. The monolithic microreactors were made of single silica rods with complex pore architecture, differing in the size of mesopores, and in particular, flow-through macropores which significantly affected the flow characteristic of the continuous system. The highest ester productivity of 105.2 mol·mol H +−1 ·h −1 was achieved in microreactor M1 with the largest porosity, characterized by a total pore volume of 4 cm 3 ·g −1 , mesopores with 20 nm diameter, and large flow-through macropores 30-50 µm in size. The strong impact of the permeability of the monoliths on a reaction kinetics was shown.

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A novel hierarchically structured siliceous packing to boost the performance of rotating bed enzymatic reactors

Cited by 21 publications

References 25 publications

Leloir Glycosyltransferases in Applied Biocatalysis: A Multidisciplinary Approach

Leloir Glycosyltransferases in Applied Biocatalysis: A Multidisciplinary Approach

Hydroxynitrile lyases covalently immobilized in continuous flow microreactors

Continuous-Flow Monolithic Silica Microreactors with Arenesulphonic Acid Groups: Structure–Catalytic Activity Relationships

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