A novel halo-alkali-tolerant and thermo-tolerant chitinase from Pseudoalteromonas sp. DC14 isolated from the Caspian Sea

Abstract

A novel halo-alkali and thermo-tolerant chitinase was obtained from an isolated strain found in the Caspian Sea. The effects of media composition and various fermentation conditions for the optimization of chitinase production were studied one factor at a time and by response surface methodology. The novel strain, which is designated as strain DC14 and phylogenetically related to the genus Pseudoalteromonas, produced chitinase after 72 h under the following optimal conditions: glucose 1 % (w/v), ammonium sulphate 0.2 % (w/v), chitin 1.07 % (w/ v), pH 8, NaCl 10 % (w/v), inoculums size 2.5 % (v/v), temperature 30°C, CaCl 2 3 mM and MgCl 2 4 mM. Using the statistical optimization method, chitinase production was found to increase from 2.30 to 21.90 U/dl. The enzyme showed maximum activity at 40°C, pH 9 and 10 % NaCl. It was stable in a wide range of temperature from 15 to 65°C, pH from pH 7 to 11 and NaCl concentration from 0 to 15 % (w/v). The molecular weight of the enzyme was estimated by SDS-PAGE to be about 65 kDa. With regard to the halo-alkali and thermo-stable properties of this enzyme, it has potential industrial activity.

A novel red pigment from marineArthrobactersp. G20 with specific anticancer activity

“…The extraction of genomic DNA, PCR amplification of 16S rRNA and phylogenetic analysis were carried out as described later (Makhdoumi et al . ).…”

“…A red-pigmented bacterium designated as strain G20 was obtained in the isolation procedure. The extraction of genomic DNA, PCR amplification of 16S rRNA and phylogenetic analysis were carried out as described later (Makhdoumi et al 2015).…”

Bioconversion of chitin and concomitant production of chitinase and N-acetylglucosamine by novel Achromobacter xylosoxidans isolated from shrimp waste disposal area

“…O-7 29 , 40 °C for Aeromonas hydrophila 30 , Pseudoalteromonas sp. DC14 31 , Pseudoalteromonas sp. DXK012 32 , Pseudomonas aeruginosa K-187 3 and 36 °C for Sphingomonas sp.…”

Isolation, identification and functional characterization of cultivable bacteria from Arabian Sea and Bay of Bengal water samples reveals high diversity

“…It also shows degradation of naphthalene, phenanthrene, dibenzothiophene and fluorene (mixed into crude oil) [45]. We have also isolated Pseudoalteromonas shioyasakiensis which was reported to show halo-alkali and thermo tolerant propertied in addition to chitinase production [46]. Same spp.…”