A novel green synthesis of silver nanoparticles by the residues of Chinese herbal medicine and their biological activities

Wei, Simin; Wang, Yinghui; Tang, Zhishu; Xu, Hongbo; Wang, Zhe; Yang, Tian; Zou, Taiyan

doi:10.1039/d0ra08287b

Cited by 33 publications

(34 citation statements)

References 45 publications

(55 reference statements)

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“…This Bragg reflection plane corresponds to the face-centered cubic (fcc) phase of silver, as previously reported. 28 The characteristics of the XRD spectrum of the NPs referenced to the JCPDS (Joint Committee on Powder Diffraction Standards -Card No. 05-0667) showed that the material had a crystalline nature.…”

Section: Resultsmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using the Flower Extract of Abelmoschus esculentus for Cytotoxicity and Antimicrobial Studies

Devanesan¹,

AlSalhi²

2021

IJN

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Background: Abelmoschus esculentus (L.) Moench, an economically important malvaceous vegetable crop popularly known as okra, is used in various culinary preparations and is rich in vitamins, minerals, and nutrients. The biological properties of okra flowers in relation to nanoparticle synthesis have not yet been reported. Materials and Methods: In the current study, silver nanoparticles (AgNPs) were synthesized using extracts of the flowers of A. esculentus. The characteristics of the AgNPs were studied using a UV-vis spectrometer, Fourier transmission infrared spectrophotometer (FTIR), X-ray diffractometer (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM) and energy dispersive X-ray spectrometer (EDX). Antibacterial activity screening was performed using the agar well diffusion method, and cytotoxicity and cell viability studies were conducted using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The synthesized AgNPs were spherical and ranged in size from 5.52 to 31.96 nm, with an average size of 16.19 nm, as determined by UV-vis spectroscopy, FTIR, XRD, TEM and EDX. A. esculentus flower extract-mediated silver nanoparticles (AME-AgNPs) exhibited excellent activities in vitro studies, particularly in vitro cytotoxic and antiproliferative studies against cancer cell lines, such as the TERT-4 and A-549 cell lines. The antibacterial effects on the Gram-positive pathogens Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus pyogenes and the Gram-negative pathogens Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Salmonella typhimurium and Shigella sonnei were tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values varied with the bacterial strain. The IC 50 values of the synthesized NPs for the tested cell lines were close to that of a standard drug. Conclusion:Compared to other NPs the NPs synthesized in this study were smaller in size and exhibited a higher level of antibacterial activity, cytotoxicity and apoptosis at minimal concentrations, and this is the first study on okra flower-induced anticancer and antimicrobial activities.

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Section: Resultsmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using the Flower Extract of Abelmoschus esculentus for Cytotoxicity and Antimicrobial Studies

Devanesan¹,

AlSalhi²

2021

IJN

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“…The AgNPs in the synthesis condition at pH 2.5 showed the smallest size, although the trend of particle size was increased with a decreasing pH solution from 11 to 3. The nonlinear relationship between pH and the particle size could be similarly observed in the synthesis of AgNPs using herbal extract [54,55]. This could be related to different charged surface on NPs in different pH [55,56].…”

Section: Effect Of the Volume Ratio Of Agno 3 To Rb Solution Onmentioning

confidence: 74%

Photoassisted Synthesis of Silver Nanoparticles Using Riceberry Rice Extract and Their Antibacterial Application

Jiamboonsri

Wanwong

2021

Journal of Nanomaterials

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The green synthesis of silver nanoparticles (AgNPs) has been attractive in biomedical applications due to its nontoxic and eco-friendly approach. This study presents the facile, rapid, and cost-effective synthesis of AgNPs by photoassisted chemical reduction using Riceberry (RB) rice extract as a reducing agent. The effects of reaction parameters including photoirradiation, irradiation time, the volume ratio of silver nitrate (AgNO3) to RB extract, and pH condition on the AgNP formation were also investigated. The characterization of AgNPs was determined by UV–visible spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier-transform infrared (FT-IR) spectroscopy. For antibacterial application, the synthesized AgNPs were studied by disc diffusion method against Escherichia coli and Staphylococcus aureus. The results indicated that light irradiation was an important factor to accelerate the formation of AgNPs. The synthesis parameters including volume of RB extract and pH condition significantly affected the particle size and crystallinity of AgNPs. The volume ratio of AgNO3 to RB extract 1 : 12.5 at pH 2.5 under photoirradiation was the successful condition to form nanometer-sized crystalline particles (average particle size of 59.48 ± 0.37 nm ) within 30 min with a rate constant of 0.210 min–1. The FT-IR measurement also suggested that the phytochemical constituents in RB extract were served as reducing and stabilizing agents for the synthesis of AgNPs. Additionally, the obtained AgNPs from various conditions demonstrated the antibacterial activity against both strains. Therefore, this study proposes an effective integration technique to synthesize AgNPs within a short time for antibacterial application.

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“…After 1.0 h of incubation, however, there were not any obvious changes in the color of the mixed solution (Figure S1). As shown in previous studies, , the formation of AgNPs would turn the color of mixed solution to dark brown. This result indicates that the primary extract of Mentha pulegium is helpless for reducing Ag + to Ag 0 within 1.0 h. This may originate from the slow formation rate, which results in very little AgNP generation.…”

Section: Resultsmentioning

confidence: 98%

Green Fabrication of Bioactive Silver Nanoparticles Using Mentha pulegium Extract under Alkaline: An Enhanced Anticancer Activity

Wang

Wei

2021

ACS Omega

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Fabrication of silver nanoparticles (AgNPs) using Chinese herbal medicine is popular as the bioactive components included in them would generate potential synergistic effect with the metal nanoparticles. The leaf of Mentha pulegium , whose extract contains a range of phytochemicals and exhibits a wide spectrum of bioactivities, is used as Chinese herbal medicine after drying naturally. Thus, the green synthesis of AgNPs using Mentha pulegium has aroused interests from analysts. However, the biosynthesis of AgNPs under alkaline conditions and the biological activities remain elusive, where alkaline conditions may influence the physicochemical properties and the biological activities of biosynthesized AgNPs. In this study, we were stimulated to fabricate bioactive AgNPs using Mentha pulegium extract under alkaline conditions, accompanied by a systematic evaluation on the effect of biosynthesis parameters on the formation, average size, and polydispersity of AgNPs. Our results showed that alkaline conditions could accelerate the formation of AgNPs with a small average size but at a disadvantage to the polydispersity. Additionally, the as-prepared AgNPs had a hexagonal structure and spherical shape with an average size of 15.7 ± 0.1 nm, existing in the monodispersed form and revealing a high degree of stability. The AgNPs exhibited potent antioxidant and significant inhibitory activity for both bacterial and cancer cell lines. The MIC values of AgNPs for Staphylococcus aureus and Escherichia coli were both 50.0 μg·mL –1 , and the IC 50 values for HCT116, HepG2, and HeLa cells were 9.0, 14.5, and 31.5 μg·mL –1 , respectively. The AgNPs biosynthesized using M. pulegium under alkaline conditions, which had a smaller size and more surface loads, are entirely different with those synthesized under acidic conditions, and the anticancer activity increased significantly. The internalization of AgNPs inside these five cells displayed a variant trend with variable AgNPs concentrations, suggesting the different mechanism of cell death. For two pathogens, HCT116 and HepG2 cancer cell lines, both cell wall and intracellular damage may be responsible for the cell death. However, for Hela cell line the cell death may be rooted in oxidative stress or intracellular penetration. These results confirmed that the AgNPs biosynthesized from M. pulegium extract under alkaline conditions would act as better anticancer agents in biomedicine.

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A novel green synthesis of silver nanoparticles by the residues of Chinese herbal medicine and their biological activities

Abstract: A novel method for green synthesis of medicinal AgNPs has been developed by using the residues of Chinese herbal medicine.

Cited by 33 publications

References 45 publications

Green Synthesis of Silver Nanoparticles Using the Flower Extract of Abelmoschus esculentus for Cytotoxicity and Antimicrobial Studies

Green Synthesis of Silver Nanoparticles Using the Flower Extract of Abelmoschus esculentus for Cytotoxicity and Antimicrobial Studies

Photoassisted Synthesis of Silver Nanoparticles Using Riceberry Rice Extract and Their Antibacterial Application

Green Fabrication of Bioactive Silver Nanoparticles Using Mentha pulegium Extract under Alkaline: An Enhanced Anticancer Activity

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