A novel grapevine badnavirus is associated with the Roditis leaf discoloration disease

Maliogka, Varvara I.; Olmos, Antonio; Pappi, Polyxeni; Lotos, Leonidas; Efthimiou, K.; Grammatikaki, G.; Candresse, Thierry; Katis, N. I.; Avgelis, A.

doi:10.1016/j.virusres.2015.03.003

Cited by 68 publications

(54 citation statements)

References 27 publications

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“…Additionally, three viroids and one virus (GRLDaV) detected in this study are reported for the first time from Croatian grapevines. Besides GBV-1, grapevine vein clearing virus (GVCV) and GRLDaV are, so far, the only known badnaviruses infecting grapevine [16,17]. Unlike GRLDaV, GBV-1 has motifs of cauliflower mosaic virus peptidase (A3); it also lacks the arginine methyltransferase-interacting protein present in GVCV.…”

Section: Discussionmentioning

confidence: 99%

Screening of some Croatian autochthonous grapevine varieties reveals a multitude of viruses, including novel ones

Vončina

Almeida

2018

Arch Virol

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Next-generation sequencing of total RNA samples from four Croatian autochthonous grapevine varieties revealed the presence of a novel virus in two grapevine accessions. The complete genome sequence of a novel virus, tentatively named "grapevine badnavirus 1" (GBV-1), was reconstructed from a de novo-assembled contig. GBV-1 has a genome of 7,145 nucleotides containing three ORFs with sequence similarity to other badnaviruses. In addition, several other viruses and viroids, including grapevine virus G, grapevine virus K/D, grapevine virus T, grapevine Roditis leaf discoloration-associated virus, grapevine yellow speckle viroids 1 and 2, and hop stunt viroid were detected and identified for the first time in Croatian grapevines in the course of this study.

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Section: Discussionmentioning

confidence: 99%

Screening of some Croatian autochthonous grapevine varieties reveals a multitude of viruses, including novel ones

Vončina

Almeida

2018

Arch Virol

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“…The genome of plant viruses can be rapidly determined even when occurring at extremely low titers in the infected host. The detection of both DNA and RNA viruses [10] has been made possible by the reconstruction of partial or complete viral genomes [11] and sequencing of the accumulated 21–24 nt virus-derived siRNAs generated by Dicer enzymes upon recognition of viral dsRNA. With the development of NGS technology and its relatively low cost, NGS has widen understanding and its potentials of diagnostics of viral pathogens without a priori knowledge of the invading pathogen, which provide accurate and timely detection of these viral pathogens in plant for effective disease management and control.…”

Section: Introductionmentioning

confidence: 99%

Ecogenomic survey of plant viruses infecting Tobacco by Next generation sequencing

Akinyemi

Wang

Zhou

et al. 2016

Virol J

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BackgroundThe invasion of plant by viruses cause major damage to plants and reduces crop yield and integrity. Devastating plant virus infection has been experienced at different times all over the world, which are attributed to different events of mutation, re-assortment and recombination occurring in the viruses. Strategies for proper virus management has been mostly limited to eradicating the vectors that spreads the plant viruses. However, development of prompt and effective diagnostic methods are required to monitor emerging and re-emerging diseases that may be symptomatic or asymptomatic in the plant as well as the genetic variation and evolution in the plant viruses. A survey of plant viruses infecting field-grown Tobacco crop was conducted in Anhui Province of China by the deep sequencing of sRNAs.MethodsSurvey of plant viruses infecting Tobacco was carried based on 104 samples collected across the province. Nine different sRNA libraries was prepared and custom-made bioinformatics pipeline coupled with molecular techniques was developed to sequence, assemble and analyze the siRNAs for plant virus discovery. We also carried out phylogenetic and recombination analysis of the identified viruses.ResultsTwenty two isolates from eight different virus species including Cucumber mosaic virus, Potato virus Y, Tobacco mosaic virus, Tobacco vein banding Mosaic virus, Pepper mottle virus, Brassica yellow virus, Chilli venial mottle virus, Broad bean wilt virus 2 were identified in tobacco across the survey area. The near-complete genome sequence of the 22 new isolates were determined and analyzed. The isolates were grouped together with known strains in the phylogenetic tree. Molecular variation in the isolates indicated the conserved coding regions have majorly a nucleotide sequence identity of 80-94 % with previously identified isolates. Various events of recombination were discovered among some of the isolates indicating that two or more viruses or different isolates of one virus infect the same host cell.ConclusionThis study describes the discovery of a consortium of plant viruses infecting Tobacco that are broadly distributed in Anhui province of China. It also demonstrates the effectiveness of NGS in identifying plant viruses without a prior knowledge of the virus and the genetic diversity that enhanced mixed infection.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-016-0639-7) contains supplementary material, which is available to authorized users.

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“…Grapevine roditis leaf discoloration-associated virus (GRlDaV) was recently found associated with typical symptoms of yellow and/or reddish discolorations and deformations of the young leaves, while grape berries are reduced in number and size, and have lower sugar content (MALIOGKA et al, 2015b). Recently the virus was also discovered in symptomless vines in apulia, Italy .…”

Section: New Emerging Diseases Of Grapevinementioning

confidence: 99%

“…Grapevine viruses associated with these diseases have a RNa genome. Recently, DNA viral agents have been identified, such as a geminivirus associated with Red blotch disease in the USA (AL RWAHNIH et al, 2013b;KRENZ et al, 2012 andPOOJaRI et al, 2013), two badnaviruses associated with Grapevine vein-clearing and vine decline syndrome in the uSa (ZhaNG et al, 2011) and with Roditis leaf discoloration disease in Greece (MALIOGKA et al, 2015b), and a unclassified virus associated with fruit tree decline syndrome in the Brazilian orchards and vineyards (BaSSO et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Recent advances in molecular biology tools to study the viral population present in vineyards, allowed the efficient detection, identification and prospection of known or new viral species and strains (al RWahNIh et al, 2015;MAREE, 2015;ROOSSINCK et al, 2015). using next-generation sequencing technologies (NGS) (al RWahNIh et al, 2015) and rolling-circle amplification (RCA) strategies (INOue-NaGaTa et al, 2004) have enhanced the diagnosis tools and knowledge of grapevine virome (al RWahNIh et al, 2013b;BaSSO et al, 2015;GIAMPETRUZZI et al, 2012;MALIOGKA et al, 2015b). In Brazilian vineyards, eighteen viruses, three viroids, two virus-like diseases and some phytoplasmas have been already reported and were molecularly characterized (BaSSO et al, 2014) (Table 2 and 3).…”

Section: Introductionmentioning

confidence: 99%

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Grapevine Virus Diseases:economic Impact and Current Advances in Viral Prospection and Management

2017

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ABSTRACT-Grapevine (Vitis spp.) is a major vegetative propagated fruit crop with high socioeconomic importance worldwide. It is susceptible to several graft-transmitted agents that cause several diseases and substantial crop losses, reducing fruit quality and plant vigor, and shorten the longevity of vines. The vegetative propagation and frequent exchanges of propagative material among countries contribute to spread these pathogens, favoring the emergence of complex diseases. Its perennial life cycle further accelerates the mixing and introduction of several viral agents into a single plant. currently, approximately 65 viruses belonging to different families have been reported infecting grapevines, but not all cause economically relevant diseases. The grapevine leafroll, rugose wood complex, leaf degeneration and fleck diseases are the four main disorders having worldwide economic importance. In addition, new viral species and strains have been identified and associated with economically important constraints to grape production. In Brazilian vineyards, eighteen viruses, three viroids and two virus-like diseases had already their occurrence reported and were molecularly characterized. Here, we review the current knowledge of these viruses, report advances in their diagnosis and prospection of new species, and give indications about the management of the associated grapevine diseases. Index terms: Vegetative propagation, plant viruses, crop losses, berry quality, next-generation sequencing. VIROSES EM VIDEIRAS: IMPACTO ECONÔMICO E RECENTES AVANÇOS NA PROSPECÇÃO DE VÍRUS E MANEJO DAS DOENÇAS DE ORIGEM VIRALRESUMO-a videira (Vitis spp.) é propagada vegetativamente e considerada uma das principais culturas frutíferas por sua importância socioeconômica mundial. ela é suscetível a vários agentes transmitidos por meio da enxertia, os quais causam diversas doenças e significativas perdas na produtividade e produção, redução na qualidade dos frutos, no vigor da planta e na longevidade dos vinhedos. A propagação vegetativa e o frequente intercâmbio de material propagativo entre países contribuem para a disseminação destes patógenos, favorecendo a emergência de doenças complexas. Seu ciclo de vida perene acelera ainda mais a mistura e a introdução de vários agentes virais em uma mesma planta. Atualmente, aproximadamente 65 vírus pertencentes a diferentes famílias foram reportados infectando videiras, embora nem todos causem doenças economicamente relevantes. as viroses do enrolamento da folha, complexo do lenho rugoso, degenerescência e mancha-das-nervuras da videira são as quatro principais desordens que têm importância econômica mundial. Além disso, novas espécies e estirpes virais foram identificadas e associadas a limitações economicamente importantes para a produção de uvas. Em vinhedos brasileiros, dezoito espécies virais, três viroides e duas doenças semelhantes a viroses já tiveram sua ocorrência reportada e foram molecularmente caracterizados. aqui, nós revisamos o conhecimento atual dessas viroses, os recentes av...

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A novel grapevine badnavirus is associated with the Roditis leaf discoloration disease

Cited by 68 publications

References 27 publications

Screening of some Croatian autochthonous grapevine varieties reveals a multitude of viruses, including novel ones

Screening of some Croatian autochthonous grapevine varieties reveals a multitude of viruses, including novel ones

Ecogenomic survey of plant viruses infecting Tobacco by Next generation sequencing

Grapevine Virus Diseases:economic Impact and Current Advances in Viral Prospection and Management

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