A Novel Glycosaminoglycan-binding Protein Is the Vertebrate Homologue of the Cell Cycle Control Protein, Cdc37

Abstract

Using a monoclonal antibody, IVd4, that recognizes a novel group of hyaluronan-binding proteins, we have immunoscreened a cDNA library constructed from embryonic chick heart muscle mRNA. One of the cDNAs isolated from the library encodes a 29.3-kDa protein homologous to Cdc37, an essential cell cycle regulatory factor previously characterized genetically in yeast and Drosophila; this is the first vertebrate CDC37 gene to be cloned to date. We also present evidence for the existence of a second chick isoform that is identical to the 29.3-kDa protein over the first 175 amino acids but is entirely different at the carboxyl terminus and lacks the IVd4 epitope. The avian Cdc37 binds hyaluronan, chondroitin sulfate and heparin in vitro, and both isoforms contain glycosaminoglycan-binding motifs previously described in several hyaluronan-binding proteins. These findings suggest a role for glycosaminoglycans in cell division control.

Hyaluronic acid induced hyaluronic acid binding protein phosphorylation and inositol triphosphate formation in lymphocytes

“…Recently, a chicken homologue of Cdc37, known to influence the activity of p34 ~a kinase in cell cycle progression is also shown to bind hyaluronic acid, heparin and chondroitin sulfate, suggesting the role of GAG binding protein in cell division control (28). Thus, the present observation documents the role of 34 kDa HA-binding protein in HA induced cellular signalling, in addition to the reported involvement of CD44 (3,4), RHAMM (5) and Cdc37 (28).…”

“…Thus, the present observation documents the role of 34 kDa HA-binding protein in HA induced cellular signalling, in addition to the reported involvement of CD44 (3,4), RHAMM (5) and Cdc37 (28). In this regard, it is important to mention that the sequence analysis of 34 kDa HA-binding protein already revealed the consensus substrate phosphorylation sites for protein kinases like PKC and CKII (8), highlighting its possible role in signalling.…”

Sequence‐specific DNA binding activity of rat cdc37‐related gene product

“…Northern analysis revealed that the radio-labeled clone 11 DNA could not detect RNA both in 3Y1 and SR-3Y1 cells, indicating that the REC11 DNA does not correspond to the exon sequence. Recently, Grammatikakis et al identified and characterized the chicken homolog of Cdc37 (CCdc37) [2]. Interestingly, CCdc37 could associate with glycosaminoglycan (GAG) and the putative GAG-binding domains were conserved among Cdc37 of different species (chicken, Drosophila and yeast).…”

“…N17 mRNA level begins to be increased in the late G1 and the same level was retained until just before the G2/M transition, suggesting the cell cycle-regulatory role(s) of N17 gene product in mammalian cell systems [1]. Interestingly, Grammatikakis et al have cloned a chicken homolog of Cdc37 (CCdc37), as one of the members which can associate with glycosaminoglycan (GAG) [2]. The nucleotide sequence (306-1047) of N17, which corresponds to the coding region of CCdc37, is 80% identical to the chicken sequence.…”

Golgi localization and dynamics of hyaluronan binding protein 1 (HABP1/p32/C1QBP) during the cell cycle

“…In this perspective, the interaction of HABP1 with LBR may also facilitate the process of chromosomal organization during mitosis. Cdc37 is a protein, which has been characterized as a hyaladherin in chicken and as an essential cell cycle regulatory protein in yeast and Drosophila [22]. The role of HABP1 in cell-adhesion is well established and in combination with HA, it might facilitate the process of adhesion and de-adhesion during mitotic stages.…”