A Novel Genetic Determination of a Lectin Gene in Iraqi Acinetobacter baumannii Isolates and Use of Purified Lectin as an Antibiofilm Agent

Muslim, Sahira Nsayef; Al-Kadmy, Israa M.S.; Auda, Ibtesam Ghadban; Ali, Alaa Naseer Mohammed; Al-Jubori, Sawsan Sajid

doi:10.5740/jaoacint.17-0422

Cited by 4 publications

(2 citation statements)

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“…Metallo ג-lactamases producing A. baumannii caused a nosocomial infection in clinical samples from patients’ sputum, blood, urine, and burn wound swabs, in addition to samples from the environment; 124 strains were acquired from Baghdad hospitals to study their multidrug resistance [ 189 ]. A study conducted by Muslim et al to determine the lectin-producing ability among 51 A. baumannii isolates from a hospital environment and patient samples of the sputum, blood, wounds and urine, revealed 17 lectin producers [ 183 ]. In addition, a study isolated 96 A. baumannii from clinical samples of wound and burn infections tested against 16 antimicrobial drugs using the disc diffusion technique, and 84 were found to be MDR [ 174 ].…”

Section: Logical Relation Between Sets Of Multidrug-resistant (Mdr) Microbesmentioning

confidence: 99%

Emerging Status of Multidrug-Resistant Bacteria and Fungi in the Arabian Peninsula

Borgio

Rasdan

Sonbol

et al. 2021

Biology

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We aimed to identify the prevalence and emerging status of multidrug-resistant bacteria and fungi and their associated mortality in nine countries in the Arabian Peninsula. Original research articles and case studies regarding multidrug-resistant bacteria and fungi in the Arabian Peninsula, published during the last 10 years, were retrieved from PubMed and Scopus. A total of 382 studies were included as per the inclusion and exclusion criteria, as well as the PRISMA guidelines, from a thorough screening of 1705 articles, in order to analyse the emerging status and mortality. The emerging nature of >120 multidrug-resistant (MDR) bacteria and fungi in the Arabian Peninsula is a serious concern that requires continuous monitoring and immediate preventive measures. More than 50% (n = 453) of multidrug-resistant, microbe-associated mortality (n = 871) in the Arabian Peninsula was due to MDR Acinetobacter baumannii, Mycobacterium tuberculosis and Staphylococcus aureus infection. Overall, a 16.51% mortality was reported among MDR-infected patients in the Arabian Peninsula from the 382 articles of this registered systematic review. MDR A. baumannii (5600 isolates) prevailed in all the nine countries of the Arabian Peninsula and was one of the fastest emerging MDR bacteria with the highest mortality (n = 210). A total of 13,087 Mycobacterium tuberculosis isolates were reported in the region. Candida auris (580 strains) is the most prevalent among the MDR fungal pathogen in the Arabian Peninsula, having caused 54 mortalities. Active surveillance, constant monitoring, the development of a candidate vaccine, an early diagnosis of MDR infection, the elimination of multidrug resistance modulators and uninterrupted preventive measures with enhanced data sharing are mandatory to control MDR infection and associated diseases of the Arabian Peninsula. Accurate and rapid detection methods are needed to differentiate MDR strain from other strains of the species. This review summarises the logical relation, prevalence, emerging status and associated mortality of MDR microbes in the Arabian Peninsula.

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Section: Logical Relation Between Sets Of Multidrug-resistant (Mdr) Microbesmentioning

confidence: 99%

Emerging Status of Multidrug-Resistant Bacteria and Fungi in the Arabian Peninsula

Borgio

Rasdan

Sonbol

et al. 2021

Biology

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“…Hemagglutinins and lectins occur in viruses, bacteria, plants, and animals. They have chemical properties that make them a helpful tool in numerous fields such as cell biology, immunology, membrane structure, molecular biology, genetic engineering, pharmacology, clinical chemistry, and cancer research [ 8 ]. The term nanoparticles refers to any particle whose three dimensions are measured on the nanoscale of atoms or molecules [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Anti‐biofilm and anti‐virulence effects of silica oxide nanoparticle–conjugation of lectin purified from Pseudomonas aeruginosa

Muslim

Ali

Auda

2021

IET Nanobiotechnology

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Pseudomonas aeruginosa lectin is purified and nanoparticle-conjugated in an attempt to inhibit biofilm formation. Thirteen (23.6%) P. aeruginosa isolates are obtained from chicken meat samples, of which 30.8% are biofilm producers and 69.2% are lectin producers. Lectin is purified 36.8-fold to final specific activity of 506.9 U/mg. Four nanoparticle types are prepared via laser ablation: platinum (Pt), gold (Au), silica oxide (SiO 2 ), and tin oxide (SnO 2 ). The four types are characterised, and pulse feeding is used to conjugate the lectin and nanoparticles. Pt, Au, SiO 2, and SnO 2 nanoparticles inhibit biofilm formation, especially SiO 2 nanoparticles, which have higher effectiveness when conjugated with purified lectin. SiO 2 -conjugated lectin significantly (p < 0.05) inhibits biofilm formation more effectively than control and other nanoparticle-conjugated lectins. Au-, Pt nanoparticle-, and SnO 2 -conjugated lectins inhibit biofilm significantly compared with control (p < 0.05), and rhlR gene expression is decreased in the presence of SiO 2 -conjugated lectin. Furthermore, lectin and Pt, Au, SiO 2 and SnO 2 nanoparticles separately, and their conjugated lectins, are effective biofilm inhibitors. Of these, SiO 2 -conjugated lectin was most significant as an anti-biofilm. Moreover, virulence factors regulon and RhlR were reduced by SiO 2conjugated lectin, indicating that this conjugation may also decrease the virulence of P. aeruginosa.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Antibacterial activity against Staphylococcus aureus of curcumin-loaded chitosan spray coupled with photodynamic treatment

Tian-mi

Zhang

Matthews

et al. 2020

LWT

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A Novel Genetic Determination of a Lectin Gene in Iraqi Acinetobacter baumannii Isolates and Use of Purified Lectin as an Antibiofilm Agent

Cited by 4 publications

References 0 publications

Emerging Status of Multidrug-Resistant Bacteria and Fungi in the Arabian Peninsula

Emerging Status of Multidrug-Resistant Bacteria and Fungi in the Arabian Peninsula

Anti‐biofilm and anti‐virulence effects of silica oxide nanoparticle–conjugation of lectin purified from Pseudomonas aeruginosa

Antibacterial activity against Staphylococcus aureus of curcumin-loaded chitosan spray coupled with photodynamic treatment

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