A novel gene trapping for identifying genes expressed under the control of specific transcription factors

Naruse, Chie; Fukusumi, Yoshiyasu; Kakiuchi, Dai; Asano, Masahide

doi:10.1016/j.bbrc.2007.06.161

Cited by 24 publications

(26 citation statements)

References 31 publications

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“…Cbx3-KO mice were generated by the gene trap method described previously (Naruse et al, 2007). To determine the genotype of mutant mice, genomic PCR was performed using Cbx3_F (5Ј-GAGTGATT -ACCGACACCACCA-3Ј) and Cbx3_R (5Ј-TTTAATCGGAGACTTG -AAGAGC-3Ј) in intron 1 for the wild-type allele, and Cbx3_F and primer 3 (5Ј-GTTCGCTTCTCGCTTCTGTT-3Ј) in the trap vector for the mutant allele.…”

Section: Micementioning

confidence: 99%

HP1γ links histone methylation marks to meiotic synapsis in mice

Takada

Naruse

Costa³

et al. 2011

Development

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SUMMARYDuring meiosis, specific histone modifications at pericentric heterochromatin (PCH), especially histone H3 tri-and dimethylation at lysine 9 (H3K9me3 and H3K9me2, respectively), are required for proper chromosome interactions. However, the molecular mechanism by which H3K9 methylation mediates the synapsis is not yet understood. We have generated a Cbx3-deficient mouse line and performed comparative analysis on Suv39h1/h2-, G9a-and Cbx3-deficient spermatocytes. This study revealed that H3K9me2 at PCH depended on Suv39h1/h2-mediated H3K9me3 and its recognition by the Cbx3 gene product HP1. We further found that centromere clustering and synapsis were commonly affected in G9a-and Cbx3-deficient spermatocytes. These genetic observations suggest that HP1/G9a-dependent PCH-mediated centromere clustering is an axis for proper chromosome interactions during meiotic prophase. We propose that the role of the HP1/G9a axis is to retain centromeric regions of unpaired homologous chromosomes in close alignment and facilitate progression of their pairing in early meiotic prophase. This study also reveals considerable plasticity in the interplay between different histone modifications and suggests that such stepwise and dynamic epigenetic modifications may play a pivotal role in meiosis.

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Section: Micementioning

confidence: 99%

HP1γ links histone methylation marks to meiotic synapsis in mice

Takada

Naruse

Costa³

et al. 2011

Development

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“…The importance of this protein is implied by knockout experiments. The WTAP‐null and heterozygous mice died at E6.5 and at E10.5, respectively . Despite its ubiquitous expression, dynamic change in its expression has been described.…”

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confidence: 99%

“…The WTAPnull and heterozygous mice died at E6.5 and at E10.5, respectively. (10,11) Despite its ubiquitous expression, dynamic change in its expression has been described. When vascular smooth muscle cells shift from a synthetic, proliferative state to a nonproliferative and contractile state, its expression was substantially upregulated.…”

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confidence: 99%

Expression and roles of Wilms' tumor 1‐associating protein in glioblastoma

et al. 2012

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Glioblastoma is a diffusely growing malignant brain tumor and among the most aggressive of all tumors. Wilms' tumor 1-associating protein (WTAP) is a nuclear protein that has been associated with regulation of proliferation and apoptosis. Although its dynamic expression and physiological functions in vascular cells have been reported, those in other cells are largely unknown. Here, we show for the first time that WTAP is overexpressed in glioblastoma. Moreover we found that WTAP regulates migration and invasion of glioblatoma cells. Specific knockdown by siRNA or overexpression by cDNA regulated migration and invasion of cancer cells. In xenograft study, WTAP overexpression made cancer cells more tumorigenic. In the investigation for its underlying mechanism, we found that the activity of epidermal growth factor receptor can be regulated by WTAP. These results reveal a novel function of WTAP and suggest its clinical application. (Cancer Sci 2012; 103: 2102-2109

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“…The cbx-3 (gene encoding HP-1γ) mutant mouse was generated by gene-trapping technology as described previously (10, 28). We found that cbx-3 − /− mice died perinatally.…”

Section: Resultsmentioning

confidence: 99%

HP-1Î³ Controls High-Affinity Antibody Response to T-Dependent Antigens

Pham

Shahsafaei

et al. 2014

Front. Immunol.

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In vitro observations suggest a role for the mouse heterochromatin protein 1γ (HP-1γ) in the immune system. However, it has not been shown if and how HP-1γ contributes to immunity in vivo. Here we show that in mice, HP-1γ positively regulates the germinal center reaction and high-affinity antibody response to thymus (T)-dependent antigens by limiting the size of CD8+ regulatory T-cell (Treg) compartment without affecting progenitor B- or T-cell-development. Moreover, HP-1γ does not control cell proliferation or class switch recombination. Haploinsufficiency of cbx-3 (gene encoding HP-1γ) is sufficient to expand the CD8+ Treg population and impair the immune response in mice despite the presence of wild-type HP-1α and HP-1β. This is the first in vivo evidence demonstrating the non-redundant role of HP-1γ in immunity.

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A novel gene trapping for identifying genes expressed under the control of specific transcription factors

Cited by 24 publications

References 31 publications

HP1γ links histone methylation marks to meiotic synapsis in mice

HP1γ links histone methylation marks to meiotic synapsis in mice

Expression and roles of Wilms' tumor 1‐associating protein in glioblastoma

HP-1Î³ Controls High-Affinity Antibody Response to T-Dependent Antigens

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