A novel gene, RSD-3/HSD-3.1, encodes a meiotic-related protein expressed in rat and human testis

Abstract

The expression of stage-specific genes during spermatogenesis was determined by isolating two segments of rat seminiferous tubule at different stages of the germinal epithelium cycle delineated by transillumination-delineated microdissection, combined with differential display polymerase chain reaction to identify the differential transcripts formed. A total of 22 cDNAs were identified and accepted by GenBank as new expressed sequence tags. One of the expressed sequence tags was radiolabeled and used as a probe to screen a rat testis cDNA library. A novel full-length cDNA composed of 2228 bp, designated as RSD-3 (rat sperm DNA no.3, GenBank accession no. AF094609) was isolated and characterized. The reading frame encodes a polypeptide consisting of 526 amino acid residues, containing a number of DNA binding motifs and phosphorylation sites for PKC, CK-II, and p34cdc2. Northern blot of mRNA prepared from various tissues of adult rats showed that RSD-3 is expressed only in the testis. The initial expression of the RSD-3 gene was detected in the testis on the 30th postnatal day and attained adult level on the 60th postnatal day. Immunolocalization of RSD-3 in germ cells of rat testis showed that its expression is restricted to primary spermatocytes, undergoing meiosis division I. A human testis homologue of RSD-3 cDNA, designated as HSD-3.1 (GenBank accession no. AF144487) was isolated by screening the Human Testis Rapid-Screen arrayed cDNA library panels by RT-PCR. The exon-intron boundaries of HSD-3.1 gene were determined by aligning the cDNA sequence with the corresponding genome sequence. The cDNA consisted of 12 exons that span approximately 52.8 kb of the genome sequence and was mapped to chromosome 14q31.3.

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 1: Background to spermatogenesis, spermatogonia, and spermatocytes

“…is a protein with 526 amino acid residues and possesses DNA-binding motifs. It is expressed in rat primary spermatocytes undergoing the first meiotic division and it is also found in human testis (Zhang et al, 2003). Hepatocyte growth factor activator inhibitor type 2 protein [ID: T1-168] is an integral membrane Kunitz-type 2 proteinase inhibitor expressed exclusively in primary spermatocytes where it may play a role during spermatogenesis (Yamauchi et al, 2002).…”

A Novel Testis-specific GTPase Serves as a Link to Proteasome Biogenesis: Functional Characterization of RhoS/RSA-14-44 in Spermatogenesis

“…In previous studies, we had identified a series of factors in spermatogenesis through a system combining laser capture microdissection (LCM) and suppressive subtractive hybridization (Zhang et al , 2003; Liang et al , 2004; Chen et al , 2008). Based on this technique, a new gene was isolated and cloned from rat testis cDNA library and assigned the name of RSA-14-44 (GenBank ID: 297173).…”

中国人类功能基因克隆与鉴定研究的文献与专利分析

“…中国医学科学院基础医学研究所克隆了一大批 和精子发生相关的基因. 例如, SPATA7 是与人睾丸 中减数分裂相关的基因 [116] ; ZYX 关系到精子成熟和 获能过程 [117] ; VCX/Y 可能参与调控精子生成过程中 核糖体的装配 [118] ; RGPD5 基因在生殖干细胞产生精 子过程中表达, 与精子发生有重要关系 [119] ; BS-63 表 达的蛋白在精子发生过程中将 aF10 蛋白运送到精子 细胞核中去, 与精子发生中有重要作用 [120] . 同时还…”