A novel gene order in the Paracentrotus lividus imtochondrial genome

Cantatore, Palmiro; Roberti, Marina; Morisco, Patrizia; Rainaldi, Guglielmo; Gadaleta, Maria Nicola; Saccone, Cecilia

doi:10.1016/0378-1119(87)90091-6

Cited by 50 publications

(25 citation statements)

References 23 publications

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“…The length is, however, normalized (318 aa) if the reading frame starts immediately downstream the tRNALe"(UUR) gene at the level of GTG. On the basis of such theoretical observations, we suggested that this non-canonical codon might act as initiator in the rat mt genome similarly to other mt genes like D. ~~&uba ND5 [23], sunflower Cyt b (Gallerani R., personal communication), sea urchin ATPase 8 [24], blue whale ND4L [25] and to some prokaryotic genes 1261. The results reported here demonstrate that there is only one mRNA for ND1 starting from GTG (Fig.…”

Section: Discussionmentioning

confidence: 99%

Transcription of rat mitochondrial NADH‐dehydrogenase subunits presence of antisense and precursor RNA species

et al. 1994

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bstract We have characterized the transcriptional pattern of the rat mitochondrial ND6containing region in vivo. We have identified a stable nyadenylated RNA species complementary for the full length of the ND6 mRNA. The analysis of the ND5 region has revealed the presence of I antisense RNA only at its 3' end. The presence of these stable antisense species complementary to structural genes is intriguing and suggests a rssible regulatory function. The quantitative analyses have demonstrated that the H transcripts, both codogenic and non-codogenic, are more stable an the L transcriptswe have defined the 5' end of the ND6 mRNA at the level of the ATG downstream of the tRNAo'". The mapping of the ND1 end has demonstrated that GTG is the first codon of the mRNA. Our findings suggest that the post-transcriptional mechanisms involved in the pression of the mt genome are much more numerous and complex than those already described in the literature.

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Section: Discussionmentioning

confidence: 99%

Transcription of rat mitochondrial NADH‐dehydrogenase subunits presence of antisense and precursor RNA species

et al. 1994

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“…These structures, however, show no primary or secondary structure similarity with each other, nor with canonical tRNAs. Moreover, the cloverleaf structures cannot be detected in the corresponding region of mtDNA of other animals (Cantatore et al, 1989;Johansen et al, 1989). The designation 'tRNA-like' for these cloverleaf structures is, therefore, doubtful.…”

Section: Generalmentioning

confidence: 99%

tRNA‐like structures

Mans

Pleij

Bosch

1991

European Journal of Biochemistry

122

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“…1), this mechanism is precluded by the fact that the two rRNA genes are separated by two proteincoding sequences (encoding NADH dehydrogenase subunits 1 and 2 [ND1 and ND2]) and by 16 tRNA genes, 12 of which are, like ND1 and ND2, transcribed off the same strand as the rRNAs (8,16,17). Furthermore, the single extended unassigned sequence found in the genome, which probably contains the replication origin and displacement (D-) loop, lies downstream, not upstream, of the small subunit (12S) rRNA gene.…”

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confidence: 99%

Mutually exclusive synthetic pathways for sea urchin mitochondrial rRNA and mRNA.

Elliott¹,

Jacobs²

1989

Mol. Cell. Biol.

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The structure and abundance of mitochondrial transcripts in sea urchin embryos were investigated by a combination of RNA blot-hybridization, SI mapping, and primer extension assays. Between the egg and blastula stages, the relative abundance of mitochondrial rRNAs declined slightly, while that of mitochondrial mRNAs increased up to 10-fold. Fine mapping of the termini of the rRNAs and of the adjacent transcripts indicated that, although they appeared to be butt-joined at their 5' ends to the upstream transcripts, tRNA-Phe 5' to the small subunit (12S) rRNA and NADH dehydrogenase subunit 2 mRNA 5' to the large subunit (16S) rRNA, respectively, their 3' ends were found to overlap the 5' ends of the downstream transcripts. 12S rRNA was found to extend 7 to 13 nucleotides into the sequence of tRNA-Glu; 16S rRNA was shown to terminate 3 to 5 nucleotides inside the coding region of cytochrome oxidase subunit I (COI) and 8 to 10 nucleotides from the mapped 5' end of COI mRNA. The rRNAs and the downstream transcripts must therefore be synthesized by distinct pathways, either by alternative processing of the same primary transcript(s) or by processing of different precursors. In either case, the events which select the ribosomal 3' ends preclude the production of functional transcripts of the downstream genes from the same precursor molecule. No developmental alterations in transcript structure were detected. We propose that mitochondrial RNA levels are regulated in early development by the selection of alternate and mutually exclusive RNA-processing pathways.Efficient protein synthesis in all cells and organelles depends on the maintenance of a large excess of rRNA over mRNA. In mammalian mitochondria, rRNA levels are up to 50 to 100 times those of individual mRNAs (2). This appears to be achieved by differential rates of synthesis of the two classes of transcript, with control exercised at the level of transcriptional termination (2, 9, 11, 13, 24). Transcription of the major coding strand of mammalian mitochondrial DNA (mtDNA) commences at the gene for tRNA-Phe (23, 24) just upstream of the effectively contiguous rRNA genes, which are separated only by tRNA-Val. Early termination of transcription at the 3' end of the large subunit (16S) rRNA gene results in the production of a subgenomic transcript which acts as a precursor to both rRNAs and probably to the two tRNAs. Relatively infrequent readthrough of this terminator is believed to result in the production of a genome-length transcript which is processed to the remaining mRNAs and tRNAs encoded on this strand.In sea urchins (Fig. 1) represented in the RNA populations of eggs and embryos (7,28), and at least one mRNA, encoding cytochrome oxidase subunit I (COI), accumulates during embryogenesis (28), whereas the level of the large subunit (16S) rRNA remains approximately constant. The ratio of mitochondrial mRNA to rRNA would therefore appear to be under developmental regulation.In order to gain insight into the mechanism by which this ratio is determined and modula...

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A novel gene order in the Paracentrotus lividus imtochondrial genome

Cited by 50 publications

References 23 publications

Transcription of rat mitochondrial NADH‐dehydrogenase subunits presence of antisense and precursor RNA species

Transcription of rat mitochondrial NADH‐dehydrogenase subunits presence of antisense and precursor RNA species

tRNA‐like structures

Mutually exclusive synthetic pathways for sea urchin mitochondrial rRNA and mRNA.

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