A novel gene, MdSSK1, as a component of the SCF complex rather than MdSBP1 can mediate the ubiquitination of S-RNase in apple

Abstract

SummaryThe related components of the SCF complex in apple were cloned and it was proved that an SCF complex containing MdSSK1 rather than MdSBP1 can mediate the ubiquitination of S-RNase.

Distinct Self-recognition in the <i>Prunus</i> S-RNase-based Gametophytic Self-incompatibility System

“…Nevertheless, the possible involvement of SBP1 in SI is still unclear because SBP1 by itself was reported to interact with S-RNase in the family Solanaceae and the tribe Maleae Kao, 2006, 2008;Minamikawa et al, 2013;Sims and Ordanic, 2001). Furthermore, polyubiquitination of S-RNase by SBP1 was observed in vitro in Solanaceae species, while no such polyubiquitination was detected in Maleae species Kao, 2006, 2008;Yuan et al, 2014).…”

“…Although SBP1 was also identified in the tribe Maleae, inconsistent results were obtained regarding the interaction between SBP1 and SFBB. Minamikawa et al (2014) observed that SBP1 interacted with SFBB and Cul1, which contradicted the findings of Yuan et al (2014). Nevertheless, the possible involvement of SBP1 in SI is still unclear because SBP1 by itself was reported to interact with S-RNase in the family Solanaceae and the tribe Maleae Kao, 2006, 2008;Minamikawa et al, 2013;Sims and Ordanic, 2001).…”

“…Pollen S F-box proteins from the families Solanaceae and Plantaginaceae and the tribe Maleae, were confirmed to form SCF complexes with the Skp1-like protein SSK1 (Huang et al, 2006;Minamikawa et al, 2014;Xu et al, 2013;Yuan et al, 2014;Zhao et al, 2010). In Solanaceae species, downregulation of either SSK1 or Cul1 results in a loss of cross-compatibility, indicating the indispensable role of the SCF SLF complex in compatible reactions, and the involvement of the UPS in S-RNase detoxification (Li and Chetlat, 2014;Zhao et al, 2010).…”

“…Several SLFs from the family Solanaceae and SFBBs from the tribe Maleae are known to interact with a subset of nonself S-RNases. Additionally, some SLFs from Solanaceae and Plantaginaceae species interact with self S-RNases (Hua and Kao, 2006;Hua et al, 2007;Kubo et al, 2010;Qiao et al, 2004a;Xu et al, 2013;Yuan et al, 2014). Hua and Kao (2006) revealed that S 2 -SLF1 has a greater affinity for S 3 -RNases than for self S-RNases, which suggests self-interactions may not be strong enough to induce polyubiquitination of self S-RNases.…”

The Jasmonate-Activated Transcription Factor MdMYC2 Regulates ETHYLENE RESPONSE FACTOR and Ethylene Biosynthetic Genes to Promote Ethylene Biosynthesis during Apple Fruit Ripening

“…The isopropyl b-D-1-thiogalactopyranoside concentration for inducing protein expression was 0.5 mM, and the purification of the GST-or His-tagged fusion proteins was performed as previously described (Li et al, 2016). The purification of the MBP-tagged fusion protein was performed as by Yuan et al (2014). All primers used are listed in Supplemental Data Set 1.…”