A novel gE-deleted pseudorabies virus (PRV) provides rapid and complete protection from lethal challenge with the PRV variant emerging in Bartha-K61-vaccinated swine population in China

Wang, Chunhua; Yuan, Jianmin; Qin, Hua-Yang; Luo, Yuzi; Xu, Cong; Li, Yongfeng; Chen, Jianing; Su, Li; Sun, Yuan; Qiu, Hua‐Ji

doi:10.1016/j.vaccine.2014.04.035

Cited by 109 publications

(102 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results demonstrated that the Bartha-K61 vaccine could effectively protect against lethal challenge with the traditional PRV SH2010 strain, but not against the emerging PRV variant strain SH2012. These findings are consistent with those of other studies [3,11].…”

Section: Discussionsupporting

confidence: 94%

“…Among these, the E43A mutation also occurred in the Bartha, Becker, and Kaplan strains [20]. Furthermore, the gC gene is most widely used for phylogenetic analysis of PRV strains mainly because the sequence is prone to variation [5,11,21]. Recent reports have revealed that PRV can be classified into two major genotypes: genotype I (European and American strains) and gen-otype II (Chinese strains) [15].…”

Section: Discussionmentioning

confidence: 99%

“…The PRV variant was shown to be both antigenically different from the classical PRVs and more pathogenic in older pigs [3,7,8,10]. Previous studies on immunological protection in sheep have shown that the Bartha-K61 vaccine does not provide complete protection against the emerging PRV variant, but could offer full protection against infection with PRV strain SC (an earlier virulent strain in China) [3,11].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Experimental Infection of Sheep With Pseudorabies Viruses Isolated In Shanghai, China, Between 2010 and 2012

Ju¹,

Yang²,

Wang³

et al. 2017

Eur J Exp Bio

View full text Add to dashboard Cite

For many years, pseudorabies has been efficiently controlled in China by vaccination. However, a sudden outbreak occurred on many pig farms during 2012-2013. In this study, phylogenetic analysis was carried out with the aim of understanding the genetic relatedness between strains in both populations and comparing strains in China with those circulating in other countries. To obtain more pathogenetic relevant data regarding two pseudorabies virus (PRV) isolates (SH2010 and SH2012) in sheep, we evaluated samples of PRV isolated in Shanghai, China between 2010 and 2012. Based on clinical signs, body temperature variation, and the maximum amounts of viral DNA in unvaccinated/infected sheep, the emerging PRV variant strain, SH2012 was observed to be more highly virulent than the traditional PRV SH2010 strain. The data of the present study will aid in a better understanding of the differences in pathogenicity between the traditional PRV SH2010 strain and the emerging PRV variant strain SH2012.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Experimental Infection of Sheep With Pseudorabies Viruses Isolated In Shanghai, China, Between 2010 and 2012

Ju¹,

Yang²,

Wang³

et al. 2017

Eur J Exp Bio

View full text Add to dashboard Cite

show abstract

“…As for PRV modified live vaccines, several virulencerelated genes such as thymidine kinase (TK), and glycoproteins glycoprotein E (gE) and glycoprotein I (gI) are commonly targeted for deletion to reduce virulence but keep immunogenicity [4] . By using bacterial artificial chromosome (BAC) technology, double-or triple-gene deleted vaccines were constructed and showed to provide useful protection as vaccine candidates [5,6] . In PRV, ribonucleotide reductase (RR) genes including RR1 (UL39) and RR2 (UL40) encode ribonucleotide reductase that reduces ribonucleoside diphosphates to the corresponding deoxyribonucleoside diphosphates and is a determinant of virus virulence in mice [7] .…”

Section: Introductionmentioning

confidence: 99%

RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies virus vaccine candidate in natural pig host

Yan¹,

He²,

Zhang³

et al. 2016

Front. Agr. Sci. Eng.

View full text Add to dashboard Cite

As virulence-determining genes, RR1 and RR2 encode the small subunit and large subunit of viral ribonucleotide reductase (RR) in pseudorabies virus which have been extensively studied in mice. However, their role in pigs has not been adequately investigated. In this study, we deleted RR1 and RR2 genes based on a TK/gE/gI triple gene-deleted pseudorabies virus and tested its efficacy in pigs as a vaccine candidate. The rescued virus showed similar growth properties and plaque size in vitro as its parent strain. In an animal study, the virus could elicit humoral immune responses shown by generation of gB-specific antibodies and virus neutralizing antibodies. However, vaccination could not provide protection against virulent pseudorabies virus challenge since vaccinated pigs showed clinical pseudorabies-specific syndromes. The deficiency in protection may due to the generation of late and low levels of gB antibodies and virus neutralizing antibodies.

show abstract

“…Sequence analysis indicated that the recently emerging PRV isolates from various regions of China were clustered into an independent branch in the phylogenetic tree, which was relatively distant from earlier ones (13)(14)(15)(16). Recently, we showed that rPRVTJ-delgE, a gE/gI-deleted PRV mutant based on the emergent PRV variant, was safe for pigs and provided complete protection against lethal challenge with the PRV variant (17). In this study, we generated a PRV variant-based recombinant expressing the CSFV E2 protein and evaluated its safety, immunogenicity, and efficacy in pigs.…”

mentioning

confidence: 97%

Generation and Efficacy Evaluation of a Recombinant Pseudorabies Virus Variant Expressing the E2 Protein of Classical Swine Fever Virus in Pigs

Wang

Yuan

et al. 2015

Clin Vaccine Immunol

Self Cite

View full text Add to dashboard Cite

Classical swine fever (CSF) is an economically important infectious disease of pigs caused by classical swine fever virus (CSFV). Pseudorabies (PR), which is caused by pseudorabies virus (PRV), is another important infectious disease of pigs and other animals. Coinfections of pigs with PRV and CSFV occur occasionally in the field. The modified live vaccine Bartha-K61 strain has played an important role in the control of PR in many countries, including China. Since late 2011, however, increasing PR outbreaks caused by an emerging PRV variant have been reported in Bartha-K61-vaccinated swine populations on many farms in China. Previously, we generated a gE/gI-deleted PRV (rPRVTJ-delgE) based on this PRV variant, which was shown to be safe and can provide rapid and complete protection against lethal challenge with the PRV variant in pigs. Here, we generated a new recombinant PRV variant expressing the E2 gene of CSFV (rPRVTJ-delgE/gI-E2) and evaluated its immunogenicity and efficacy in pigs. The results showed that rPRVTJ-delgE/gI-E2 was safe for pigs, induced detectable anti-PRV and anti-CSFV neutralizing antibodies, and provided complete protection against the lethal challenge with either the PRV TJ strain or the CSFV Shimen strain. The data indicate that rPRVTJ-delgE/gI-E2 is a promising candidate bivalent vaccine against PRV and CSFV coinfections.

show abstract

A novel gE-deleted pseudorabies virus (PRV) provides rapid and complete protection from lethal challenge with the PRV variant emerging in Bartha-K61-vaccinated swine population in China

Cited by 109 publications

References 18 publications

Experimental Infection of Sheep With Pseudorabies Viruses Isolated In Shanghai, China, Between 2010 and 2012

Experimental Infection of Sheep With Pseudorabies Viruses Isolated In Shanghai, China, Between 2010 and 2012

RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies virus vaccine candidate in natural pig host

Generation and Efficacy Evaluation of a Recombinant Pseudorabies Virus Variant Expressing the E2 Protein of Classical Swine Fever Virus in Pigs

Contact Info

Product

Resources

About