The effect of seasonal and geographical changes on a nutritional yield of Tribulus terrestris fruits was investigated in the South Indian region. Principally important nutritive elements like carbohydrates, crude protein, and crude fiber were studied in this investigation. The study evaluated two seasonal and three geographically variable Tribulus terrestris fruits collected from South India (Karnataka, Tamilnadu and Telangana states) in June and December months during 2015 for analysis of crude protein, crude fiber, and carbohydrates. The values of the specifications determined were significantly greater for the December season than that of samples collected in June season. Fruits collected from Bellary (Karnataka state) region recorded higher quantity of nutritional elements than the fruits collected from Telangana and Tamilnadu regions. It was concluded that the variation in the nutritional quality of Tribulus terrestris fruits collected between the main June, December season and three different geographical locations is presented. The findings showed that the nutritive values of Tribulusfruits were remarkably altered as a result of seasonal and geographical variability.