A novel function of CD82/KAI-1 on E-cadherin-mediated homophilic cellular adhesion of cancer cells

Abé, Masakazu; Sugiura, Tetsuro; Takahashi, Miho; Ishii, Kazuya; Shimoda, Miyuki; Shirasuna, Kanemitsu

doi:10.1016/j.canlet.2008.02.058

Cited by 62 publications

(73 citation statements)

References 22 publications

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“…The results of the in vitro invasion assay showed a significant induction of cancer cell dissemination from the primary tumor nests in the ACCS-M GFP cell line. As this assay evaluates E-cadherin-mediated tumor cell dissemination (14), these data support the invasive and metastatic character of the primary implanted tumor of ACCS-M GFP in vivo and suggest that molecular alteration of cell adhesion molecules occurs on ACCS-M GFP cell surfaces. One explanation for the success of in vivo selection and changes in metastatic character is the inherent genetic heterogeneity of malignant cells.…”

Section: Discussionsupporting

confidence: 59%

“…Tumor dissemination potential from the primary cancer nest was evaluated by an in vitro invasion assay, as described previously (14). briefly, 1x10…”

Section: Accs Metastatic Orthotopic Implantation Mouse Model and In Vmentioning

confidence: 99%

“…To evaluate the invasive and metastatic characteristics of the ACCS cell lines, we performed a newly established invasion assay using fibroblast-containing collagen type I, which mimics cancer mesenchymal tissue, as described previously (14). In this assay, cancer cells are allowed to form highly concentrated tumor nests, and subsequent cell migration through the ECM, which closely mimics the conditions found in vivo, is measured.…”

Section: Analysis Of Invasive and Metastatic Characteristics Of Highlmentioning

confidence: 99%

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Involvement of epithelial-mesenchymal transition in adenoid cystic carcinoma metastasis

et al. 2011

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Abstract. The high frequencies of recurrence and distant metastasis of adenoid cystic carcinoma (AdCC) are significant obstacles for the long-term cure of patients with AdCC and emphasize the need for better understanding of the biological factors associated with these outcomes. To identify proteins that mediate AdCC metastasis, we established three AdCC cell lines expressing green fluorescent protein (GFP) from the ACCS cell line using orthotopic transplantation and in vivo selection in nude mice: Parental ACCS-GFP, highly tumorigenic ACCS-T GFP and metastatic ACCS-M GFP. ACCS-GFP and ACCS-M GFP were subjected to DNA microarray analysis and the results were used for data mining studies. DNA microarray analysis revealed significantly altered biological processes in the ACC-M GFP cells, including events related to cell adhesion (three categories) and signaling (three categories). In particular, a significant down-regulation of cell adhesion molecules, such as cadherins and integrin subunits was observed. The loss of E-cadherin and integrins and the gain of vimentin in ACCS-M GFP cells were confirmed by immunoblotting. These results suggest that epithelial-mesenchymal transition (EMT) is a putative event in AdCC metastasis that induces tumor cell dissemination from the primary tumor site. In summary, in this study we established a useful nude mouse metastasis model which will enable further AdCC metastasis research and clinical treatment trials and we also provide evidence that EMT is significantly involved in the AdCC metastatic process.

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Section: Discussionsupporting

confidence: 59%

“…Tumor dissemination potential from the primary cancer nest was evaluated by an in vitro invasion assay, as described previously (14). briefly, 1x10…”

Section: Accs Metastatic Orthotopic Implantation Mouse Model and In Vmentioning

confidence: 99%

Section: Analysis Of Invasive and Metastatic Characteristics Of Highlmentioning

confidence: 99%

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Involvement of epithelial-mesenchymal transition in adenoid cystic carcinoma metastasis

et al. 2011

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“…The question as to how CD82, a tumor suppressor protein, mediates xenorecognition is yet to be addressed. Tumor metastasis suppression by CD82 is thought to occur by stabilizing E-cadherin/b-catenin complex formation (66), upregulation of Sprouty2 (67), maturation of b 1 -integrin (68), and direct interactions with the Duffy Ag/receptor for chemokine-expressing endothelial cells (69). Whether such mechanisms could be exploited for increased survival of a xenograft remains to be addressed.…”

Section: Discussionmentioning

confidence: 99%

Identification of the Tetraspanin CD82 as a New Barrier to Xenotransplantation

Saleh

Parhar

Al-Hejailan

et al. 2013

The Journal of Immunology

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Significant immunological obstacles are to be negotiated before xenotransplantation becomes a clinical reality. An initial rejection of transplanted vascularized xenograft is attributed to Galα1,3Galβ1,4GlcNAc-R (Galα1,3-Gal)–dependent and –independent mechanisms. Hitherto, no receptor molecule has been identified that could account for Galα1,3-Gal–independent rejection. In this study, we identify the tetraspanin CD82 as a receptor molecule for the Galα1,3-Gal–independent mechanism. We demonstrate that, in contrast to human undifferentiated myeloid cell lines, differentiated cell lines are capable of recognizing xenogeneic porcine aortic endothelial cells in a calcium-dependent manner. Transcriptome-wide analysis to identify the differentially expressed transcripts in these cells revealed that the most likely candidate of the Galα1,3-Gal–independent recognition moiety is the tetraspanin CD82. Abs to CD82 inhibited the calcium response and the subsequent activation invoked by xenogeneic encounter. Our data identify CD82 on innate immune cells as a major “xenogenicity sensor” and open new avenues of intervention to making xenotransplantation a clinical reality.

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“…CD82 inhibits fibronectin adhesion-induced epithelial-to-mesenchymal transition in prostate cancer cells by repressing the associated integrin signaling [3], and CD44 alternative splicing-dependent melanoma metastasis by mediating U2AF2 ubiquitination and degradation [4]. Abe et al [5] found that CD82 strengthened E-cadherin-mediated intercellular adhesion, stabilized E-cadherin/ β-catenin complex formation, and reduced tyrosine phosphorylation of β-catenin on HGF stimulation. CD82 specifically suppressed ubiquitylation of EGFR after stimulation with heparin-binding EGF or amphiregulin [6], and attenuated compartmentalisation and ligand-induced dimerization of EGFR [7].…”

Section: Introductionmentioning

confidence: 99%

The roles of CD82 expression in gastric cancer: a meta and bioinformatics analysis

Zheng¹,

Gong²

2017

Oncotarget

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CD82 encodes a transmembrane glycoprotein of tetraspanins family, and functions as a tumor metastasis suppressor. We performed a systematic meta and bioinformatics analysis through multiple online databases up to March 14, 2017. We¬ found down-regulated CD82 expression in gastric cancer, compared with normal mucosa (p < 0.05). CD82 expression was negatively with depth of invasion, lymph node and distant metastasis, TNM staging and dedifferentiation of gastric cancer (p < 0.05). A positive association between CD82 expression and favorable overall survival was found in patients with gastric cancer (p < 0.005). According to bioinformatics analysis, CD82 mRNA expression was higher in gastric cancer than normal tissues (p < 0.05). According to Kaplan-Meier plotter and TCGA database, we found that a higher CD82 expression was positively correlated with overall or progression-free survival rates of all cancer patients, even stratified by aggressive parameters or as an independent factor (p < 0.05). These findings indicated that CD82 expression might be employed as a potential marker to indicate gastric carcinogenesis and subsequent progression, even favorable prognosis.

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A novel function of CD82/KAI-1 on E-cadherin-mediated homophilic cellular adhesion of cancer cells

Cited by 62 publications

References 22 publications

Involvement of epithelial-mesenchymal transition in adenoid cystic carcinoma metastasis

Involvement of epithelial-mesenchymal transition in adenoid cystic carcinoma metastasis

Identification of the Tetraspanin CD82 as a New Barrier to Xenotransplantation

The roles of CD82 expression in gastric cancer: a meta and bioinformatics analysis

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