A novel function for KIF13B in germ cell migration

Tarbashevich, Katsiaryna; Dzementsei, Aliaksandr; Pieler, Tomas

doi:10.1016/j.ydbio.2010.10.016

Cited by 24 publications

(46 citation statements)

References 49 publications

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“…7B). efnb1 inhibition significantly increased the total number of embryos containing mislocalized PGCs according to the boundaries designated for normal PGC localization, between somites 5 and 11, as described by Tarbashevich et al (2011). Mislocalization was along the anterior/posterior (A/P) axis, primarily beyond the normal posterior boundary (Fig.…”

Section: Misexpression Of Sox7 Reduces Pgc Numbermentioning

confidence: 78%

High-throughput analysis reveals novel maternal germline RNAs crucial for primordial germ cell preservation and proper migration

et al. 2017

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During oogenesis, hundreds of maternal RNAs are selectively localized to the animal or vegetal pole, including determinants of somatic and germline fates. Although microarray analysis has identified localized determinants, it is not comprehensive and is limited to known transcripts. Here, we utilized high-throughput RNAsequencing analysis to comprehensively interrogate animal and vegetal pole RNAs in the fully grown Xenopus laevis oocyte. We identified 411 (198 annotated) and 27 (15 annotated) enriched mRNAs at the vegetal and animal pole, respectively. Ninety were novel mRNAs over 4-fold enriched at the vegetal pole and six were over 10-fold enriched at the animal pole. Unlike mRNAs, microRNAs were not asymmetrically distributed. Whole-mount in situ hybridization confirmed that all 17 selected mRNAs were localized. Biological function and network analysis of vegetally enriched transcripts identified protein-modifying enzymes, receptors, ligands, RNA-binding proteins, transcription factors and co-factors with five defining hubs linking 47 genes in a network. Initial functional studies of maternal vegetally localized mRNAs show that sox7 plays a novel and important role in primordial germ cell (PGC) development and that ephrinB1 (efnb1) is required for proper PGC migration. We propose potential pathways operating at the vegetal pole that highlight where future investigations might be most fruitful.

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Section: Misexpression Of Sox7 Reduces Pgc Numbermentioning

confidence: 78%

High-throughput analysis reveals novel maternal germline RNAs crucial for primordial germ cell preservation and proper migration

et al. 2017

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“…Our yeast two-hybrid screen using a gastrula cDNA library identified the motor kinesin KIF13B as a Dvl2 interacting partner (see Materials and Methods). KIF13B, containing the microtubule-binding CAP-Gly domain, has been previously implicated in neuronal polarity (Horiguchi et al, 2006) and the migration of germ cells in Xenopus embryos (Tarbashevich et al, 2011). We confirmed the biochemical interaction of Dvl2 and KIF13B by immunoprecipitation of tagged proteins from lysates of transfected HEK293T cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The following morpholino oligonucleotides (MOs) have been used: Vangl2/Stbm MO 5′-GAG TAC CGG CTT TTG TGG CGA TCCA-3′ (Ossipova et al, 2015a), KIF13B MO, 5′-ATCTTGCACAGCGAGCTCCCCTAAC-3′ (Tarbashevich et al, 2011); Pk3 MO1, 5′-GGATGCCGCCCGCTCTCTCCCTTA-3′. Pk3 MO2, 5′-CTCCTCCTGGAATTACGGAACATCC-3′.…”

Section: Methodsmentioning

confidence: 99%

“…To further address the underlying mechanism, we identified the motor kinesin KIF13B as a Disheveled-interacting protein. KIF13B has been previously implicated in cell polarity and cell migration (Horiguchi et al, 2006; Tarbashevich et al, 2011). We demonstrate that KIF13B physically associates with Disheveled and synergizes with PCP signaling to regulate cell intercalatory behavior.…”

Section: Introductionmentioning

confidence: 99%

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The involvement of PCP proteins in radial cell intercalations during Xenopus embryonic development

Ossipova

Chu

Fillâtre

et al. 2015

Developmental Biology

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The planar cell polarity (PCP) pathway orients cells in diverse epithelial tissues in Drosophila and vertebrate embryos and has been implicated in many human congenital defects and diseases, such as ciliopathies, polycystic kidney disease and malignant cancers. During vertebrate gastrulation and neurulation, PCP signaling is required for convergent extension movements, which are primarily driven by mediolateral cell intercalations, whereas the role for PCP signaling in radial cell intercalations has been unclear. In this study, we examine the function of the core PCP proteins Vangl2, Prickle3 (Pk3) and Disheveled in the ectodermal cells, which undergo radial intercalations during Xenopus gastrulation and neurulation. In the epidermis, multiciliated cell (MCC) progenitors originate in the inner layer, but subsequently migrate to the embryo surface during neurulation. We find that the Vangl2/Pk protein complexes are enriched at the apical domain of intercalating MCCs and are essential for the MCC intercalatory behavior. Addressing the underlying mechanism, we identified KIF13B, as a motor protein that binds Disheveled. KIF13B is required for MCC intercalation and acts synergistically with Vangl2 and Disheveled, indicating that it may mediate microtubule-dependent trafficking of PCP proteins necessary for cell shape regulation. In the neural plate, the Vangl2/Pk complexes were also concentrated near the outermost surface of deep layer cells, suggesting a general role for PCP in radial intercalation. Consistent with this hypothesis, the ectodermal tissues deficient in Vangl2 or Disheveled functions contained more cell layers than normal tissues. We propose that PCP signaling is essential for both mediolateral and radial cell intercalations during vertebrate morphogenesis. These expanded roles underscore the significance of vertebrate PCP proteins as factors contributing to a number of diseases, including neural tube defects, tumor metastases, and various genetic syndromes characterized by abnormal migratory cell behaviors.

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“…PGCs explanted from Xenopus embryos on the other hand, exhibit bleb-like protrusions [17] that appear to be powered by cytoplasmic flow rather than by actin polymerization [18]. Likewise, zebrafish PGC migration is facilitated by the formation of actin-free protrusions in the form of blebs that are inflated in the direction of migration [19].…”

Section: Introductionmentioning

confidence: 99%