A novel form of TNF/cachectin is a cell surface cytotoxic transmembrane protein: Ramifications for the complex physiology of TNF

Kriegler, Michael; Perez, Carl F.; DeFay, Kim; Albert, Iris; Lu, Shi-Da

doi:10.1016/0092-8674(88)90486-2

Cited by 993 publications

(493 citation statements)

References 26 publications

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“…The immunologic importance of the cell-associated cytokines is largely unknown. On the other hand, IL-1, 23 TNF, 24 LT␣ 25 and IFN␥ 26 are expressed in two different versions, one is a membrane-bound form and the other is soluble form. While precise functional discrimination of the forms has not been made, the membrane-bound form of TNF has a unique function distinct from that of the soluble TNF.…”

Section: Figure 3 Proliferation Of T Cell Clones In Response Tomentioning

confidence: 99%

“…Several cytokines are expressed as membrane-associated forms, soluble forms or proteolytically processed forms; IL-1, 23 TNF, 24 LT␣, 25 and IFN␥. 26 Genetically engineered membrane-bound forms can result in lower tox- icity of TNF and LT␣.…”

Section: Introductionmentioning

confidence: 99%

“…26 Genetically engineered membrane-bound forms can result in lower tox- icity of TNF and LT␣. 24,25 In some cases cytokines secreted by the tumor cells bind to receptors on the tumor cells and deliver effective signals by cell to cell contact. 27,28 In the present study, we have generated transfected tumor cells expressing a membrane-bound form of IL-4 (IL-4m).…”

Section: Introductionmentioning

confidence: 99%

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Tumor cells expressing membrane-bound form of IL-4 induce antitumor immunity

Kim

Sonn

et al. 2000

Gene Ther

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Local cytokine concentrations are required for inhibition of tumor growth with less toxic side-effects. However, genetically engineered tumor cells secreting cytokines still induce toxicity and activate bystander cells. To circumvent such problems, membrane-bound forms of IL-4 (IL-4m) were expressed on MethA fibrosarcoma tumor cells. Chimeric forms of IL-4 with the type I transmembrane protein CD4 or type II transmembrane protein TNF were designed to express IL-4 in opposite orientations on the tumor cell surface. The IL-4m on tumor clones was able to support cell growth of the IL-4 dependent cytotoxic cell line (CT.4S) and

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Section: Figure 3 Proliferation Of T Cell Clones In Response Tomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tumor cells expressing membrane-bound form of IL-4 induce antitumor immunity

Kim

Sonn

et al. 2000

Gene Ther

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“…However, TNF-␣ is known to be produced as a membrane bound precursor, which requires proteolytic activation. 43 In order to find out whether TNF-␣ is released from NB4 cells, we determined soluble TNF-␣ in NB4 conditioned medium, which is a prequisite for the autocrine regulation we describe here.…”

Section: Figurementioning

confidence: 99%

Autocrine regulation of matrix metalloproteinase-9 gene expression and secretion by tumor necrosis factor-α (TNF-α) in NB4 leukemic cells: specific involvement of TNF receptor type 1

Ismair¹,

Ries²,

Lottspeich

et al. 1998

Leukemia

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“…IL-4 modulates IgE levels, resulting in inflammatory cell migration to inter-respiratory cells [8], and IL-13 induces morphological changes in the pulmonary system typical of asthma, such as mucous hypersecretion, epithelial hyperplasia, base membrane thickening, inflammatory cell infiltration, and B cell activation [9–12]. TNF-α is produced by macrophages and is involved in the interaction between mast cells [13] and airway smooth muscle cells, a key mechanism for inducing airway hyperresponsiveness [14]. In asthma, IL-6 is upregulated in pulmonary epithelial cells by various stimuli [15] and is involved in facilitating IL-4 differentiation, downregulating Th2 cell differentiation, and promoting Th17 cell differentiation [16].…”

Section: Introductionmentioning

confidence: 99%

Opuntia humifusamodulates morphological changes characteristic of asthma via IL-4 and IL-13 in an asthma murine model

Lee

Bae

Choi

et al. 2017

Food & Nutrition Research

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Asthma is a chronic pulmonary disease that affects an estimated 235 million people worldwide, but asthma drugs have many adverse effects. Opuntia humifusa (eastern prickly pear) has been used as a food and traditional medicine worldwide; however, its anti-asthmatic effects have not been reported. We evaluated O. humifusa as a potential therapeutic or preventive component of anti-asthmatic drugs. We divided ovalbumin-sensitized mice into the following groups: normal control, asthma-induced control, dexamethasone-treated group (positive control), 50 mg/kg O. humifusa-treated group, 100 mg/kg O. humifusa-treated group, and 500 mg/kg O. humifusa-treated group. Levels of Th1/Th2/Th17-related cytokines were evaluated using RT-PCR, ELISA, and immunohistochemistry. O. humifusa dose-dependently suppressed the morphological changes typically observed in asthma, such as goblet cell hyperplasia, inflammatory cell infiltration, mucous hypersecretion, and relative basement membrane thickening in the respiratory system. These results may be attributable to regulation of Th1-/Th2-/Th17-related factors, especially interleukin (IL)-4 and IL-13. We conclude that O. humifusa is a potential anti-asthmatic functional food. Abbreviations: O. humifusa: Opuntia humifusa; Th: helper T; RT-PCR: real-time polymerase chain reaction; ELISA: enzyme-linked immunosorbent assay; IL: interleukin; WHO: World Health Organization; IFN-γ: interferon gamma; TNF-α: tumor necrosis factor-alpha; IgE: immunoglobulin E; CD: cluster of differentiation; OVA: ovalbumin; DEX: dexamethasone; BALF: bronchoalveolar fluid; H&E: hematoxylin and eosin; PAS: periodic acid-schiff; PBS: phosphate-buffered saline; BM: basement membrane; cDNA: complementary deoxyribonucleic acid; RNA: ribo nucleic acid; RIPA: radioimmunoprecipitation assay; IHC: immunohistochemistry; HPLC: high-performance liquid chromatography; SD: standard deviation; WBC: white blood cells; APCs: antigen-presenting cells

show abstract

A novel form of TNF/cachectin is a cell surface cytotoxic transmembrane protein: Ramifications for the complex physiology of TNF

Cited by 993 publications

References 26 publications

Tumor cells expressing membrane-bound form of IL-4 induce antitumor immunity

Tumor cells expressing membrane-bound form of IL-4 induce antitumor immunity

Autocrine regulation of matrix metalloproteinase-9 gene expression and secretion by tumor necrosis factor-α (TNF-α) in NB4 leukemic cells: specific involvement of TNF receptor type 1

Opuntia humifusamodulates morphological changes characteristic of asthma via IL-4 and IL-13 in an asthma murine model

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