A novel fluorescent turn-on probe for highly selective detection of nitroreductase in tumor cells

Zhu, Kangning; Qin, Tianyi; Zhao, Chen; Luo, Zijie; Huang, Yingying; Liu, Bin; Wang, Lei

doi:10.1016/j.snb.2018.08.134

Cited by 37 publications

(13 citation statements)

References 39 publications

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“…2 × 10 6 cells were suspended in media and implanted subcutaneously into nude mice. A549 and A549/DDP xenografts were established in nude mice until the tumor volumes typically reached to about 200 mm 3 . All experiments were performed in accordance with the guidelines established by the Committee of Animal Research Policy of Binzhou Medical University.…”

Section: Establishment Of the A549 And A549/ddp Transplanted Tumor Numentioning

confidence: 99%

“…Encouraged by the results that HBO-assistant chemotherapy can effectively induce tumor cell apoptosis and can effectively treat tumor cells, we then evaluated the therapy efficacy of HBO-assistant chemotherapy in A549 xenografts mice. A549 xenografts were established in nude mice until tumor volume typically reached approximately to 200 mm 3 [43]. Next, tumor-bearing nude mice were divided into two groups.…”

Section: Therapy Efficacy Of Hbo-assistant Chemotherapy In A549 Xenogmentioning

confidence: 99%

“…[1] Tumor hypoxia has been known as a pathophysiological consequence of deteriorating microenvironment, and it is mainly derived from the rapid and exaggerated growth of tumor cells [2]. Hypoxia can lead to structures, functions and diffusion conditions of tumors disturbed, and it plays significant roles in tumor invasion, metastasis and drug resistance [3]. Researchers report that hypoxia causes many tumor-associated genes overexpress, especially hypoxia inducible factors 1α (HIF-1α) and HIF-mediated genes [4,5,48].…”

Section: Introductionmentioning

confidence: 99%

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Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo

Wang

Zhang

Huang

et al. 2020

Sensors and Actuators B: Chemical

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Tumor resistance is a huge challenge for tumor treatment, which may lead to tumor treatment failure. Relieving tumor resistance and improving efficacy are long-term goals for tumor treatment. Nitroreductase (NTR) is an endogenous enzyme that highly expressed in hypoxia tumors. Herein, we develop a NTR-activatable fluorescent probe, TP-NO 2 , for NTR detection during tumor treatment. TP-NO 2 with simple synthesis steps and high yield can qualitatively and quantitatively detect NTR in a wide range (0−20 μg/mL) with a detection limit of 26 ng/ mL. The probe has been successfully applied for NTR detection in cells under simulated hypoxia conditions. Hyperbaric oxygen (HBO) can alleviate tumor hypoxia and reduce NTR concentration. We pre-evaluate the effect of tumor treatment by NTR imaging. And the results demonstrate that HBO-assistant chemotherapy can effectively treat tumor cells. We further apply TP-NO 2 for NTR detection in A549 and cis-dichlorodiamineplatinum(II) (DDP)-resistant A549 (A549/DDP) xenograft nude mice, and we choose NTR as an indicator to pre-evaluate the treatment efficacy of malignant tumors. With the adjuvant therapy of HBO, chemotherapeutic drugs gemcitabine and carboplatin can effectively treat A549 and A549/DDP xenograft. These applications provide us a novel perspective for NTR imaging.

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Section: Establishment Of the A549 And A549/ddp Transplanted Tumor Numentioning

confidence: 99%

Section: Therapy Efficacy Of Hbo-assistant Chemotherapy In A549 Xenogmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo

Wang

Zhang

Huang

et al. 2020

Sensors and Actuators B: Chemical

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“…The flexibility of these enzymes comprises their usefulness in a variety of biological and medical applications. These include environmental decontamination using bioremediation [ 2 ]; various cancer therapies such as gene and viral-directed prodrugs [ 3 , 4 , 5 , 6 , 7 ] and probes for detecting hypoxia in tumours [ 8 , 9 , 10 , 11 ], antiparasitics [ 12 , 13 ], herbicides [ 14 ] and for the detection of explosives [ 15 ]. For a more detailed review of the many uses of nitroreductases, see the papers by Kumari et al [ 11 ], Zhang et al [ 4 ] and Nepali et al [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

The Role of Nitroreductases in Resistance to Nitroimidazoles

Thomas

Gwenin

2021

Biology

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Antimicrobial resistance is a major challenge facing modern medicine, with an estimated 700,000 people dying annually and a global cost in excess of $100 trillion. This has led to an increased need to develop new, effective treatments. This review focuses on nitroimidazoles, which have seen a resurgence in interest due to their broad spectrum of activity against anaerobic Gram-negative and Gram-positive bacteria. The role of nitroreductases is to activate the antimicrobial by reducing the nitro group. A decrease in the activity of nitroreductases is associated with resistance. This review will discuss the resistance mechanisms of different disease organisms, including Mycobacterium tuberculosis, Helicobacter pylori and Staphylococcus aureus, and how these impact the effectiveness of specific nitroimidazoles. Perspectives in the field of nitroimidazole drug development are also summarised.

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“…However, most of these NTR fluorescent probes need ultraviolet light as the excitation light which could cause photodamage to biological samples. Most importantly, only a few fluorescent probes have the ability to target the activity of NTR in specific subcellular organelle [ 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel NIR Fluorescent Probe for Highly Selective Detection of Nitroreductase and Hypoxic-Tumor-Cell Imaging

Liu

Zhang

et al. 2021

Molecules

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Nitroreductase as a potential biomarker for aggressive tumors has received extensive attention. In this work, a novel NIR fluorescent probe for nitroreductase detection was synthesized. The probe Py-SiRh-NTR displayed excellent sensitivity and selectivity. Most importantly, the confocal fluorescence imaging demonstrated that HepG-2 cells treated with Py-SiRh-NTR under hypoxic conditions showed obvious enhanced fluorescence, which means that the NTR was overexpressed under hypoxic conditions. Moreover, the probe showed great promise that could help us to study related anticancer mechanisms research.

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A novel fluorescent turn-on probe for highly selective detection of nitroreductase in tumor cells

Cited by 37 publications

References 39 publications

Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo

Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo

The Role of Nitroreductases in Resistance to Nitroimidazoles

A Novel NIR Fluorescent Probe for Highly Selective Detection of Nitroreductase and Hypoxic-Tumor-Cell Imaging

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