A novel fluorescent probe with large Stokes shift for the detection of viscosity changes and its imaging in living cells

Abstract

As an important cellular microenvironmental parameter, viscosity could reflect the status of living cells. Small molecular fluorescent probes are a vital tool to measure the change of viscosity in living cells. A novel fluorescence probe ZL-1 with a large Stokes shift (in methanol it reached to 153 nm and in glycerol it reached to 125 nm) and excellent sensitivity toward viscosity was developed. The sharp enhancement of the emission intensity for the probe ZL-1 from low viscous methanol to high viscous glycerol indicated that the probe ZL-1 could respond to the viscosity variations.Moreover, the probe ZL-1 has been successfully utilized to detect of the viscosity variations in living cells.

Simultaneous detection of SO2 and viscosity in drug‐induced inflammation in live cells and zebrafish

“…This phenomenon is a stress response and homeostasis mechanism that can cause organelle breakdown and bodily dysfunction [12–14]. Severe abnormalities in viscosity are associated with various diseases, including but not limited to inflammation, fatty liver, and so on [15–17]. A susceptible and non‐invasive imaging tool holds significant potential for elucidating the mechanisms underlying related diseases and discerning the biological and pathological functions associated with viscosity is paramount.…”

“…with various diseases, including but not limited to inflammation, fatty liver, and so on [15][16][17]. A susceptible and non-invasive imaging tool holds significant potential for elucidating the mechanisms underlying related diseases and discerning the biological and pathological functions associated with viscosity is paramount.…”