A novel fluorescent adhesive-assisted biomimetic mineralization

Abstract

We propose a novel fluorescent adhesive-assisted biomimetic mineralization strategy, based on which 1 wt% of sodium fluorescein and 25 wt% of polyacrylic acid stabilized amorphous calcium phosphate (PAA-ACP) nanoparticles were incorporated into a mild self-etch adhesive (Clearfil S3 Bond) as a fluorescent mineralizing adhesive. The characterization of the PAA-ACP nanoparticles indicates that they were spherical particles clustered together, each particle with a diameter of approximately 20-50 nm, in a metastable phase with two characteristic absorption peaks (1050 cm-1 and 580 cm-1). Our results suggest that the fluorescent mineralizing adhesive was non-cytotoxic with minimal esthetic interference and its fluorescence intensity did not significantly decrease within 6 months. Our data reveal that the fluorescent mineralizing adhesive could induce the extra- and intra-fibrillar remineralization of the reconstituted type I collagen, the demineralized enamel and dentin substrate. Our data demonstrate that a novel fluorescent adhesive-assisted biomimetic mineralization strategy will pave the way to design and produce anti-carious materials for the prevention of dental caries.

The use of bioactive particles and biomimetic analogues for increasing the longevity of resin-dentin interfaces: A literature review

“…More than ten different analogs have been tested for dentin remineralization 47) . Among them, those tested for resin-dentin interfaces remineralization include PAA 9,[48][49][50][51][52][53][54][55][56] , polyvinylphosphonic acid (PVA) 9,[53][54][55] , sodium trimetaphosphate (STMP) [48][49][50][51]57,58) , polyaspartic acid (PAS) 57,58) , and other engineered peptides [59][60][61] . The majority of the studies added these analogs to the storage medium (usually SBF or AS) 9,17,48,[50][51][52][53][54][55][56] , while few studies employed them as water-based primers [57][58][59]61) , and one study tested an experimental adhesive containing ACP nanoparticles stabilized by PAA 52) .…”

In vitro remineralization of enamel white spot lesions with a carrier-based amorphous calcium phosphate delivery system

“…Similar to CPP, PAA is a biomimetic analogue that can stabilize ACP precursors and induce them to remineralize dentin and enamel. 17,36 However, in order to make PAA-ACP more stable and periodically replenished, a mesoporous carrier would be highly desirable. Mesoporous silica nanoparticles (MSN) are remarkable biological delivery systems for a variety of drugs due to their large pore volume, high internal surface area, and cost effectiveness.…”

Effect of polyvinylphosphonic acid on resin-dentin bonds and the cytotoxicity of mouse dental papilla cell-23

“…Biomimetic remineralization, an approach that mimics natural biomineralization, has been reported to play a crucial role in inducing amorphous calcium phosphate nanoparticles to remineralize type I collagen and improve adhesive procedures. 7,8 The evidence suggests that the application of the protease inhibitors might be less effective in promoting dentin remineralization. 23 In a previous study, the use of biomimetic remineralized materials provided durable resin-dentin bonds.…”

“…4,5 Biomimetic dentin remineralization, a valuable tool for maintaining the mechanical properties and biostability of collagen fibrils, is an emerging biomedical technology for improving the durability of the bond strength. [6][7][8] Remineralization of demineralized dentin may reduce collagen degradation, [9][10][11] and specific agents have been used for this purpose.…”