A novel fluorescence immunoassay based on inner filter effect and gold nanoclusters for Vibrio parahaemolyticus determination

Yang, Gangyi; Wei, Chunhao; Tang, Yunyu; Zhang, Jingru; Zhang, Xuan; Chi, Hai; Lei, Tao; Kong, Cong

doi:10.1016/j.rechem.2021.100208

Cited by 4 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…16−19 Novel methods recent in the literature include colorimetric aggregation assays 20 and fluorescent nanoclusters in enzymelinked immuno sorbent assay (ELISA). 21 Here, we describe an inexpensive and ready-to-use immunochromatographic assay to detect Vp in raw oysters (Figure 1). These assays possess the advantages of being lowcost and easy to use and can be administered at POC.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Thus, providing a point of consumption (POC) assay that can be read out without special training or instruments is critical to contribute to the prevention of vibriosis. Nanotechnology has enabled novel sensing methods for various diseases and biomarkers and thus has the potential to facilitate new modes of detection of these pathogens. − Novel methods recent in the literature include colorimetric aggregation assays and fluorescent nanoclusters in enzyme-linked immuno sorbent assay (ELISA) …”

Section: Introductionmentioning

confidence: 99%

“… 16 − 19 Novel methods recent in the literature include colorimetric aggregation assays 20 and fluorescent nanoclusters in enzyme-linked immuno sorbent assay (ELISA). 21 …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Gold Nanoparticle Paper Immunoassays for Sensing the Presence of Vibrio parahaemolyticus in Oyster Hemolymph

et al. 2023

View full text Add to dashboard Cite

Seafood contamination with Vibrio bacteria is a problem for aquaculture, especially with oysters, which are often consumed raw. Current methods for diagnosing bacterial pathogens in seafood involve lab-based assays such as polymerase chain reaction or culturing, which are time consuming and must occur in a centralized location. Detection of Vibrio in a point-of-care assay would be a significant tool for food safety control measures. We report here a paper immunoassay that can detect the presence of Vibrio parahaemolyticus (Vp) in buffer and oyster hemolymph. The test uses gold nanoparticles conjugated to polyclonal anti-Vibrio antibodies in a paper-based sandwich immunoassay. A sample is added to the strip and wicked through by capillary action. If Vp is present, it results in a visible color at the test area that can be read out by eyes or a standard mobile phone camera. The assay has a limit of detection of 6.05 × 10 5 cfu/mL and a cost estimate of $5 per test. Receiver operating characteristic curves with validated environmental samples showed a test sensitivity of 0.96 and a specificity of 1.00. Because the assay is inexpensive and can be used on Vp directly without the requirement for culturing, or sophisticated equipment, it has the potential to be used in fieldable settings.

show abstract

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Gold Nanoparticle Paper Immunoassays for Sensing the Presence of Vibrio parahaemolyticus in Oyster Hemolymph

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Zeng et al [15] reported the development of a PCR-based lateral flow test strip for detecting V. parahaemolyticus in codfish, with a detection limit of 50 CFU/mL. Similarly, Yang [16] developed a fluorescence immunoassay based on the inner filter effect and BSA-gold nanoclusters. Each of these methods has advantages and disadvantages, and developing new methods for the rapid and sensitive detection of V. parahaemolyticus is crucial for the surveillance of foodborne diseases.…”

Section: Introductionmentioning

confidence: 99%

Detection of Vibrio parahaemolyticus Based on Magnetic and Upconversion Nanoparticles Combined with Aptamers

Song,

Li,

et al. 2023

Foods

View full text Add to dashboard Cite

Vibrio parahaemolyticus is a halophilic and heat-labile gram-negative bacterium and is the most prevalent foodborne bacterium in seafood. In order to develop a rapid and sensitive method for detecting the foodborne pathogenic bacterium Vibrio parahaemolyticus, an aptamer-modified magnetic nanoparticle and an aptamer-modified upconversion nanoparticle were synthesised and used as a capture probe and a signal probe, respectively. The aptamer-modified magnetic nanoparticle, V. parahaemolyticus cell, and aptamer-modified upconversion nanoparticle formed a sandwich-like complex, which was rapidly separated from a complex matrix using a magnetic force, and the bacterial concentration was determined by fluorescence intensity analysis. The results showed that the fluorescence intensity signal correlated positively with the concentration of V. parahaemolyticus in the range of 3.2 × 102 to 3.2 × 105 CFU/mL, with a linear equation of y = 296.40x − 217.67 and a correlation coefficient of R2 = 0.9610. The detection limit of the developed method was 4.4 CFU/mL. There was no cross-reactivity with other tested foodborne pathogens. This method is highly specific and sensitive for the detection of V. parahaemolyticus, and can achieve the qualitative detection of this bacterium in a complex matrix.

show abstract

Highly efficient dual-mode detection of AFB1 based on the inner filter effect: Donor-acceptor selection and application

Xiong,

Sun,

Zhang

et al. 2024

Analytica Chimica Acta

View full text Add to dashboard Cite

A novel fluorescence immunoassay based on inner filter effect and gold nanoclusters for Vibrio parahaemolyticus determination

Cited by 4 publications

References 22 publications

Gold Nanoparticle Paper Immunoassays for Sensing the Presence of Vibrio parahaemolyticus in Oyster Hemolymph

Gold Nanoparticle Paper Immunoassays for Sensing the Presence of Vibrio parahaemolyticus in Oyster Hemolymph

Detection of Vibrio parahaemolyticus Based on Magnetic and Upconversion Nanoparticles Combined with Aptamers

Highly efficient dual-mode detection of AFB1 based on the inner filter effect: Donor-acceptor selection and application

Contact Info

Product

Resources

About