“…Immunomagnetic enrichment was carried out as described previously . Briefly, 5 mL of each blood sample was lysed by lysing solution (BD Biosciences, San Jose, CA, USA), and then was negatively enriched using Dynabeads coated with anti‐CD45 mAb (Thermo Fisher Scientific Inc., Waltham, MA, USA) to remove white blood cells, followed by fixation and labeling with the FITC‐conjugated anti‐CK mAb CK3‐6H5 (1:25 dilution; Miltenyi Biotec, Bergisch‐Gladbach, Germany), the phycoerythrin‐conjugated anti‐vimentin mAb D21H3 (1:50 dilution; Cell Signaling Technology, Danvers, MA, USA), and the Alexa Fluor 700‐conjugated anti‐CD45 mAb F10‐89‐4 (1:20 dilution; AbD Serotec, Oxford, UK).…”