A Novel Ferroptosis Related Gene Signature for Prognosis Prediction in Patients With Colon Cancer

Abstract

PurposeColon cancer (CC) is a serious disease burden. The prognosis of patients with CC is different, so looking for effective biomarkers to predict prognosis is vitally important. Ferroptosis is a promising therapeutic and diagnosis strategy in CC. However, the role of ferroptosis in prognosis of CC has not been studied. The aim of the study is to build a prognosis model related ferroptosis, and provide clues for further therapy of CC.MethodsThe RNA-seq data were from TCGA (training group) and GEO (testing group). The R language and Perl language were used to process and analyze data. LASSO regression analysis was used to build the prognosis model. ssGSEA was used to compare the immune status between two groups. Immunohistochemistry was used to detect expression of AKR1C1 and CARS1 in colon cancer tissues and adjacent tissues.ResultsThe prognosis model consisted of five ferroptosis related genes (AKR1C1, ALOX12, FDFT1, ATP5MC3, and CARS1). The area under curve (AUC) at 1-, 2-, and 3-year were 0.668, 0.678, and 0.686, respectively. The high- and low-risk patients had significant survival probability and could be clearly distinguished by the PCA and t-SNE analysis. The multivariate cox regression analysis also showed the riskscore is an independent prognosis factor. Importantly, we found that the immune status between high- and low-risk patients were different obviously, such as CD8+T cells. And STING, a new promising immune target, was also correlated to our signature genes statistically significantly.ConclusionOur ferroptosis prognosis signature could predict survival of CC patients to a certain degree. And the crosstalk between ferroptosis and immune, especially STING need further studies.

An Original Ferroptosis-Related Gene Signature Effectively Predicts the Prognosis and Clinical Status for Colorectal Cancer Patients

“…Immunotherapy combined with ferroptosis-targeting therapy might be a feasible therapeutic approach. Almost at the same time with our work, there were some studies reported the prognostic signature of ferroptosis-related genes for colon cancer patients ( 69 , 70 ). Compared to their works, this signature in our study demonstrated better predictive ability.…”

Construction and Validation of a Novel Ferroptosis-Related lncRNA Signature to Predict Prognosis in Colorectal Cancer Patients

“…Through the above analysis, we preliminarily inferred that these four ferroptosis-related lncRNAs may directly or indirectly regulate DE-FGs or genes participated in the pathways which were closely related to CRC and thus caused the difference in patient survival. Nie et al (2021) comprehensively reported construction of a ferroptosis related genes prognosis model in colon cancer, which aimed to predict survival probability of patients. Our study further screened for differently expressed lncRNAs in CRC which were associated with ferroptosis-related genes, and then constructed a ferroptosis-related lncRNA prognosis model of CRC by Cox regression analyses.…”

A New Prognostic Risk Signature of Eight Ferroptosis-Related Genes in the Clear Cell Renal Cell Carcinoma

“…Down regulation of ACACA expression is associated with the inhibition of malignant progression of prostate cancer (27). Nie et al constructed a novel prognostic signature involving CARS1, which effectively predicted the prognosis of colon cancer (28). CRYAB has been reported to be a potential therapeutic target for nasopharyngeal carcinoma (29).…”