A novel feedstock for biodiesel production: The application of palmitic acid from Schizochytrium

Chen, Wei; Ma, Lin; Zhou, Pengpeng; Zhu, Yongming; Wang, Xiaopeng; Luo, Xin-an; Bao, Zhendong; Yu, Liang

doi:10.1016/j.energy.2015.03.110

Cited by 42 publications

(13 citation statements)

References 63 publications

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“…Nonetheless, Schizochytrium sp. S056 almost met the EN 14214 standard (Chen et al 2015 ), showing that thraustochytrid crude oil rich in palmitic acid could potentially be used for biodiesel exploitation directly without requiring a winterization step. However, the CFPP (7.5 °C) was higher when compared to the findings of this study (as low as −10.9 °C) which would be a disadvantage (Table 6 ).…”

Section: Resultsmentioning

confidence: 91%

“…Most of the research on DHA from thraustochytrids to date has been performed with sub-tropical strains, many of which have been isolated from Malaysian, Japanese and Chinese coastal waters (Fan et al 2002 ; Li et al 2009 ; Byreddy et al 2015 ; Chen et al 2015 ; Manikan et al 2015 ). Other work has been done on isolates from temperate regions such as southern Australian waters and cold temperate waters of Canadian littoral (Burja et al 2006 ; Lee Chang et al 2012 ).…”

Section: Introductionmentioning

confidence: 99%

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Screening of new British thraustochytrids isolates for docosahexaenoic acid (DHA) production

et al. 2017

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Thraustochytrids isolated from hot tropical and sub-tropical waters have been well studied for DHA and biodiesel production in the last decades. However, little research has been performed on the oils of cold water thraustochytrids, in particular from the North Sea region. In this study, thraustochytrid strains from British waters showed high relative levels of omega-3 long-chain (≥C20) polyunsaturated fatty acids (LC-PUFA), including docosahexaenoic acid (DHA, 22:6ω3). The relative levels of DHA (as % of total fatty acids, TFA) in the different British strains are hitherto amongst the highest recorded from any thraustochytrid screening study, with strain TL18 reaching up to 67% DHA in modified Glucose-Yeast Extract-Peptone (GYP) medium. At this screening stage, low final biomass and fatty acid yield were observed in modified GYP and MarChiquita-Brain Heart Broth (MCBHB), while PUFA profiles (as % of PUFA) remained unaltered regardless of the culture medium used. Hence, optimizing the medium and culture conditions to improve growth and lipid content, without impacting the relative percentage of DHA, has the potential to increase the final DHA concentration. With this in mind, three strains were identified as promising organisms for the production of DHA. In the context of possible future industrial exploitation involving a winterization step, we investigated the recycling of the residual oil for biodiesel use. To do this, a mathematical model was used to assess the intrinsic properties of the by-product oil. The results showed the feasibility of producing primary DHA-rich oil, assuming optimized conditions, while using the by-product oil for biodiesel use.

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Section: Resultsmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Screening of new British thraustochytrids isolates for docosahexaenoic acid (DHA) production

et al. 2017

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“…The thraustochytrid strains Schizochytrium sp. S056 [28] and Schizochytrium limacinum [27] have been considered for biodiesel production due to their FA profiles and rapid growth patterns. The Icelandic T. kinnei isolates from Location A had similar FA profiles as these strains, but in order for them to be considered for biofuel production their growth must be further optimized.…”

Section: Discussionmentioning

confidence: 99%

“…Lipid profiles with high ratios of long chains (C13–C19) of saturated and monounsaturated fatty acids have better fuel properties than high ratios of very long chains (≥ C20) (e.g., [24,25,26]). Thraustochytrid species have been considered for biodiesel production as strains that synthesize fatty acids (FAs) have been isolated to produce high-quality fuel [27,28].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters

et al. 2019

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The following study reports on the first thraustochytrid isolates identified from Iceland. They were collected from three different locations off the northern coast of the country (Location A, Skagaströnd; Location B, Hveravík; and Location C, Eyjafjörður). Using 18S rDNA sequence analysis, isolates from Locations A and B were identified within the Thraustochytrium kinnei species while other isolates within the Sicyoidochytrium minutum species when compared to other known strains. Cells isolated from Locations A ( 2 . 10 ± 0 . 70 g/L) and B ( 1 . 54 ± 0 . 17 g/L) produced more biomass than the ones isolated from Location C ( 0 . 43 ± 0 . 02 g/L). This study offers the first-time examination of the utility of byproducts from fisheries as a nitrogen source in media formulation for thraustochytrids. Experiments showed that isolates produced more biomass (per unit of substrate) when cultured on nitrogen of marine ( 2 . 55 ± 0 . 74 g/L) as compared to of commercial origin ( 1 . 06 ± 0 . 57 g/L). Glycerol ( 2 . 43 ± 0 . 56 g/L) was a better carbon source than glucose ( 1 . 84 ± 0 . 57 g/L) in growth studies. Fatty acid (FA) profiles showed that the isolates from Location C (S. minutum) had low ratios of monounsaturated ( 4 . 21 ± 2 . 96 % ) and omega-6 ( 0 . 68 ± 0 . 59 % ) FAs. However, the isolates also had high ratios of docosahexaenoic acid (DHA; 35 . 65 ± 1 . 73 % ) and total omega-3 FAs ( 40 . 39 ± 2 . 39 % ), indicating that they could serve as a source of marine oils for human consumption and in aquaculture feeds. The T. kinnei isolates from Location A could be used in biodiesel production due to their high ratios of monounsaturated ( 18 . 38 ± 6 . 27 % ) long chain ( 57 . 43 ± 8 . 27 % ) FAs.

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“…Furthermore, the biomass of thraustochytrids may be used as feed for marine fish and poultry to improve the quality of the products (Burja et al 2006;Singh et al 2014;Ueda et al 2015). Moreover, thraustochytrids have been found to be involved in biodiesel production, which could help to alleviate energy shortages (Chen et al 2015(Chen et al , 2020.…”

Section: Introductionmentioning

confidence: 99%

Cultivation and diversity analysis of novel marine thraustochytrids

Liu

Wang

2020

Mar Life Sci Technol

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Thraustochytrids are a group of unicellular marine heterotrophic protists, and have long been known for their biotechnological potentials in producing squalene, polyunsaturated fatty acids (PUFAs) and other bioactive products. There are less than a hundred known strains from diverse marine habitats. Therefore, the discovery of new strains from natural environments is still one of the major limitations for fully exploring this interesting group of marine protists. At present, numerous attempts have been made to study thraustochytrids, mainly focusing on isolating new strains, analyzing the diversity in specific marine habitats, and increasing the yield of bioactive substances. There is a lack of a systematic study of the culturable diversity, and cultivation strategies. This paper reviews the distribution and diversity of culturable thraustochytrids from a range of marine environments, and describes in detail the most commonly used isolation methods and the control of culture parameters. Furthermore, the perspective approaches of isolation and cultivation for the discovery of new strains are discussed. Finally, the future directions of novel marine thraustochytrid research are proposed. The ultimate goal is to promote the awareness of biotechnological potentials of culturable thraustochytrid strains in industrial and biomedical applications.

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A novel feedstock for biodiesel production: The application of palmitic acid from Schizochytrium

Cited by 42 publications

References 63 publications

Screening of new British thraustochytrids isolates for docosahexaenoic acid (DHA) production

Screening of new British thraustochytrids isolates for docosahexaenoic acid (DHA) production

Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters

Cultivation and diversity analysis of novel marine thraustochytrids

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