A novel fed-batch process based on the biology of Aurantiochytrium sp. KRS101 for the production of biodiesel and docosahexaenoic acid

Kim, Kyochan; Kim, Eun Jung; Ryu, Byung Gon; Park, Soojung; Choi, Yoon E.; Yang, Ji Won

doi:10.1016/j.biortech.2012.10.139

Cited by 55 publications

(23 citation statements)

References 24 publications

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“…Kim et al (2015) suggested that increasing the concentration of sea salt from 2 g/L to 20 g/L reduced cell rupturing of Aurantiochytrium sp. KRS101 and augmented biomass from 13.61 to 17.31 g/L 27 .…”

Section: Resultsmentioning

confidence: 97%

Co-production of DHA and squalene by thraustochytrid from forest biomass

Patel

Liefeldt

Rova

et al. 2020

Sci Rep

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Omega-3 fatty acids, and specifically docosahexaenoic acid (DHA), are important and essential nutrients for human health. thraustochytrids are recognised as commercial strains for nutraceuticals production, they are group of marine oleaginous microorganisms capable of co-synthesis of DHA and other valuable carotenoids in their cellular compartment. the present study sought to optimize DHA and squalene production by the thraustochytrid Schizochytrium limacinum SR21. The highest biomass yield (0.46 g/g substrate) and lipid productivity (0.239 g/g substrate) were observed with 60 g/L of glucose, following cultivation in a bioreactor, with the DHA content to be 67.76% w/w total lipids. to reduce costs, cheaper feedstocks and simultaneous production of various value-added products for pharmaceutical or energy use should be attempted. To this end, we replaced pure glucose with organosolv-pretreated spruce hydrolysate and assessed the simultaneous production of DHA and squalene from S. limacinum SR21. After the 72 h of cultivation period in bioreactor, the maximum DHA content was observed to 66.72% w/w total lipids that was corresponded to 10.15 g/L of DHA concentration. While the highest DHA productivity was 3.38 ± 0.27 g/L/d and squalene reached a total of 933.72 ± 6.53 mg/L (16.34 ± 1.81 mg/ g CDW). In summary, we show that the co-production of DHA and squalene makes S. limacinum SR21 appropriate strain for commercial-scale production of nutraceuticals. Omega-6 (n-6) and omega-3 (n-3) are polyunsaturated fatty acids (PUFAs), whose precursors include linoleic acid and alpha-linolenic acid. They are considered essential because humans cannot synthesize them due to insufficient levels of elongases and delta-6-desaturases 1,2. Although conversion of alpha-linolenic acid into omega-3 fatty acids, such as eicosapentaenoic acid (C 20:5n−3), docosapentaenoic acid (C 22:5n−3), and docosahexaenoic acid (C 22:6n−3 , DHA) occurs in humans, it happens at a very slow rate 3 and so these fatty acids must be provided by the diet. These PUFAs have several health benefits and have been found to be very effective in the preventions and treatment of fetal diseases 4. DHA plays a major role in cell signalling and is found mainly in the brain and retina tissues 5. Additionally, it acts as an anti-inflammatory agent, a precursor of several metabolites and potent lipid mediators, and could be used to treat several cardiovascular or neurologic disorders, such as hypertension and Alzheimer's 6. Fish of the Salmonidae, Scombridae, and Clupeidae families have been the sole commercial source of DHA as their oil contains approximately 20 to 30% DHA 3. However, DHA purification and concentration from fish oil is costly and the resulting oil quality depends on species, location, and pollution 7. Furthermore, fish-derived DHA is unsuitable for vegetarians, and diminishing fish stocks together with marine pollution limit the increasing demand for DHA 7. Oils from genetically engineered plant oilseeds, such as Brassica juncea, Arabidopsis thaliana, an...

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Section: Resultsmentioning

confidence: 97%

Co-production of DHA and squalene by thraustochytrid from forest biomass

Patel

Liefeldt

Rova

et al. 2020

Sci Rep

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“…Crude glycerol derived from the biodiesel production process probably contains several potentially toxic compounds, which would be inhibitive to cell growth when using high concentration feeding [23]. Similar to exponential feeding by varying the feeding rate, a novel modified fed-batch cultivation method was developed for the cultivation of Aurantiochytrium sp., in which nitrogen and carbon sources were supplied at different time intervals [24]. This method increased biomass, palmitic acid, and DHA production 2-to 4-fold and utilized carbon and nitrogen sources more efficiently compared with the yields obtained using batch and non-modified fed-batch methods.…”

Section: Effects Of Feeding Criteria On the Growth Of R Glutinis In mentioning

confidence: 93%

The effects of feeding criteria on the growth of oleaginous yeast—Rhodotorula glutinis in a pilot-scale airlift bioreactor

Yen

Liu

Chang

2015

Journal of the Taiwan Institute of Chemical Engineers

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“…In addition to lipids, thraustochytrids have also been reported to produce carotenoid pigments, carbohydrates, proteins and several degrading enzymes . Recently, researchers altered the fatty acid compositions of lipids of Aurantiochytrium using different compositions of the nutrient medium . The major advantage of thraustochytrids is that they grow fast and attain high cell density within a short period of time.…”

Section: Oleaginous Microorganismsmentioning

confidence: 99%

“…[53][54][55] Recently, researchers altered the fatty acid compositions of lipids of Aurantiochytrium using different compositions of the nutrient medium. 56 The major advantage of thraustochytrids is that they grow fast and attain high cell density within a short period of time. However, thraustochytrids require a large quantity of nutrients, especially glucose, for their growth.…”

Section: Oleaginous Fungimentioning

confidence: 99%

Role of flow cytometry for the improvement of bioprocessing of oleaginous microorganisms

Sonowal

Chikkaputtaiah

Velmurugan

2019

J of Chemical Tech & Biotech

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Oleaginous microorganisms, such as yeast, fungi, microalgae and bacteria, represent a key segment of second generation feed‐stock materials and are considered to synthesize a wide range of industrially important chemical compounds. Oleaginous microorganisms possess a broad varieties of chemical compounds such as carotenoids, pigments, carbohydrates, chlorophyll, and storage‐material lipids. Oleaginous microorganisms have been recognized as promising sources for the synthesis of unsaturated, especially polyunsaturated fatty acids (PUFA). So far, a variety of high‐throughput screening methodologies (HTMs) have been employed for the development of bioprocessing of oleaginous microorganisms for sustainable production of industrially valuable compounds. Of HTMs, flow cytometry (FC) and sorters (FACS) have received substantial interest as better HTMs because of their ability to screen large numbers of cells within seconds, and interrogate and isolate living cells at single‐cell level. Forward and side scattering signals of FC are used to determine the physiological state of the cell while different channels available in the FC facilitate the detection of signals produced from fluorophores. Simultaneous measurement of physiological characteristics along with specific compound accumulation at single‐cell level enables the possibility of separating a particular phenotype with specific properties from a population. Different microbial strain development strategies in combination with FACS produced improved phenotypes with desired properties. This review first summarizes the FACS methodologies suitable for oleaginous microorganisms and the significant progress that has been achieved in oleaginous microorganisms using FACS, and highlights the important, advanced and future prospects of FACS methodologies that are suitable for the development of bioprocessing in oleaginous microorganisms. © 2018 Society of Chemical Industry

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A novel fed-batch process based on the biology of Aurantiochytrium sp. KRS101 for the production of biodiesel and docosahexaenoic acid

Cited by 55 publications

References 24 publications

Co-production of DHA and squalene by thraustochytrid from forest biomass

Co-production of DHA and squalene by thraustochytrid from forest biomass

The effects of feeding criteria on the growth of oleaginous yeast—Rhodotorula glutinis in a pilot-scale airlift bioreactor

Role of flow cytometry for the improvement of bioprocessing of oleaginous microorganisms

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