A novel family of genomic resistance islands, AbGRI2, contributing to aminoglycoside resistance in Acinetobacter baumannii isolates belonging to global clone 2

Nigro, Steven J.; Farrugia, Daniel N.; Paulsen, Ian T.; Hall, Ruth M.

doi:10.1093/jac/dks459

Cited by 66 publications

(61 citation statements)

References 20 publications

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“…IS26 is encountered with increasing frequency in plasmids of clinical importance where it is involved in expression of antibiotic resistance genes and plasmid rearrangements (166)(167)(168)(169)(170)(171).…”

Section: Is6 (I) Generalmentioning

confidence: 99%

Everyman's Guide to Bacterial Insertion Sequences

et al. 2015

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The number and diversity of known prokaryotic insertion sequences (IS) have increased enormously since their discovery in the late 1960s. At present the sequences of more than 4000 different IS have been deposited in the specialized ISfinder database. Over time it has become increasingly apparent that they are important actors in the evolution of their host genomes and are involved in sequestering, transmitting, mutating and activating genes, and in the rearrangement of both plasmids and chromosomes. This review presents an overview of our current understanding of these transposable elements (TE), their organization and their transposition mechanism as well as their distribution and genomic impact. In spite of their diversity, they share only a very limited number of transposition mechanisms which we outline here. Prokaryotic IS are but one example of a variety of diverse TE which are being revealed due to the advent of extensive genome sequencing projects. A major conclusion from sequence comparisons of various TE is that frontiers between the different types are becoming less clear. We detail these receding frontiers between different IS-related TE. Several, more specialized chapters in this volume include additional detailed information concerning a number of these.In a second section of the review, we provide a detailed description of the expanding variety of IS, which we have divided into families for convenience. Our perception of these families continues to evolve and families emerge regularly as more IS are identified. This section is designed as an aid and a source of information for consultation by interested specialist readers.

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Section: Is6 (I) Generalmentioning

confidence: 99%

Everyman's Guide to Bacterial Insertion Sequences

et al. 2015

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“…The aacC1 and aadA1 genes are located in a class 1 integron in the cassette arrangement of aacC1-orfP-orfP-orfQ-aadA1. AbGRI1, found in comM of GC2 isolates, has a different structure than AbaR islands and contains different resistance genes (6,11,12).…”

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confidence: 99%

“…1A). AbGRI2 is bounded by copies of IS26 and has variable gene content (11). AbGRI3 is another IS26-bounded region that contains the 16S methylase gene, armA, which confers resistance to all clinically used aminoglycosides (13).…”

mentioning

confidence: 99%

“…AbGRI2-1 was the first version of AbGRI2 described in detail and was found in Australian isolates WM99c from 1999 and A91 from 2005 (11). A related IS26-bounded structure, AbGRI2-2, was present in a Chinese isolate, MDR-TJ (14).…”

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confidence: 99%

“…AbGRI2-1 contains aphA1b in Tn6020 and bla TEM as well as a class 1 integron with the same cassette arrangement as in AbaR islands, aacC1-orfP-orfP-orfQaadA1 and sul1 in the 3=-conserved sequence (3=-CS). In addition to having the same cassettes, another segment of AbGRI2-1 is identical to a region in AbaR islands (11). Further versions of AbGRI2 that have been reported recently vary in size as well as the resistance genes they contain (11,13,15).…”

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confidence: 99%

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Evolution of AbGRI2-0, the Progenitor of the AbGRI2 Resistance Island in Global Clone 2 of Acinetobacter baumannii

Blackwell

Nigro

Hall

2016

Antimicrob Agents Chemother

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A320, isolated in the Netherlands in 1982 and also known as RUH134, is the earliest available multiply antibiotic-resistant (MAR) Acinetobacter baumannii isolate belonging to global clone 2 (GC2) and is the reference strain for this clone. The draft genome sequence of A320 was used to investigate the original location and configuration of the IS26-bounded AbGRI2 resistance island found in current GC2 isolates. PCR mapping and sequencing were used to order contigs composing the resistance islands. A320 contains two IS26-bounded resistance islands, AbGRI2-0a and AbGRI2-0b, of 7.8 kb and 25.4 kb, respectively. Together they contain bla TEM , aacC1, aadA1, sul1, catA1, and aphA1b genes, which confer resistance to antibiotics used clinically in the 1970s, as well as an incomplete mercury resistance module. Tracking the continuity of the chromosome and the target site duplications revealed that the two resistance islands were originally together as AbGRI2-0, an island of 32.4 kb, and were subsequently separated via an IS26-mediated intramolecular inversion that reversed the orientation of 1.54 Mb of the chromosome and duplicated an IS26. A320 contains an ancestral form of AbGRI2, and the original insertion site of the AbGRI2 island was identified. Many of the AbGRI2 versions present in the completed GC2 genomes can be derived from it via the variant AbGRI2-1. IS26-mediated inversions have also played a part in forming AbGRI2-0, and, upon reversal, large regions of AbGRI2-0 are identical to parts of AbaR0, the ancestral version of the AbaR islands present in GC1 isolates. This indicates a common source.

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