A novel family 9 β-1,3(4)-glucanase from thermoacidophilic Alicyclobacillus sp. A4 with potential applications in the brewing industry

Bai, Yingguo; Wang, Jianshe; Zhang, Zhifang; Shi, Pengjun; Luo, Huiying; Huang, Huoqing; Luo, Chunliang; Yao, Bin

doi:10.1007/s00253-010-2452-3

Cited by 43 publications

(17 citation statements)

References 27 publications

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“…Exogenous enzymes, usually β-glucanases, have been used to improve lautering performance (9)(10)(11)(12)(13)(14)(15). Depending on the difference in substrate selectivity, GH family origin, and presence of inhibitors and modular architecture, xylanases have different degradation behaviour towards arabinoxylan (16).…”

Section: Introductionmentioning

confidence: 99%

Secretome analysis of Trichoderma reesei CICC41495 for degradation of arabinoxylan in malted barley

Sun

Xie

2018

J. Inst. Brew.

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Arabinoxylan, one of the major non‐starch polysaccharides of the endosperm cell wall of barley, has a negative impact on the filterability of wort and beer. The addition of microbial xylanase would be an effective strategy to degrade arabinoxylan. In this study, the secretome of Trichoderma reesei CICC 41495 cultivated under submerged fermentation with wheat bran as a carbon source was characterised by two‐dimensional electrophoresis combined with MALDI‐TOF/TOF tandem mass spectrometry. A total of 24 proteins were identified in the culture supernatant, 23 of which were involved in non‐starch polysaccharide degradation. The proteins belonged to 10 different families of glycoside hydrolases, including xyloglucanase, cellobiohydrolase I, cellobiohydrolase II, endoglucanase II, endo‐1,4‐β‐glucanase VII, β‐glucosidase, endo‐1,4‐β‐xylanase I and II, endo‐1,4‐β‐xylanase III, and α‐l‐arabinofuranosidase. Of these, four were correlated with arabinoxylan degradation. Supplementation of the secretome loading of 0.14 mg protein/g malt to mash resulted in the total degradation of high molecular weight arabinoxylan, a 90% increase in filtration rate and a 7.9% decrease in viscosity. The results suggest that T. reesei produces a tailored xylanolytic enzyme system that efficiently hydrolysed high molecular weight arabinoxylan. Accordingly, the secretome of this fungi has potential value in the brewing industry. © 2018 The Institute of Brewing & Distilling

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Section: Introductionmentioning

confidence: 99%

Secretome analysis of Trichoderma reesei CICC41495 for degradation of arabinoxylan in malted barley

Sun

Xie

2018

J. Inst. Brew.

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“…Among them, endo-1,3-1,4-b-glucanases and endo-1,3(4)-b-glucanases have been widely applied as industrial enzymes in the brewing process to reduce the viscosity and filtration time of mash and in animal feed to improve the digestibility of b-glucan [2,7,38]. A number of bacterial glucanases have been reported from Streptomyces [33], Fibrobacter succinogenes [36], Bacillus [30,37], and Paenibacillus [10,39].…”

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confidence: 99%

High-level expression of a novel Penicillium endo-1,3(4)-β-d-glucanase with high specific activity in Pichia pastoris

Chen

Meng

Shi

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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A novel endo-1,3(4)-β-D-glucanase gene (bgl16C1) from Penicillium pinophilum C1 was cloned and sequenced. The 945-bp full-length gene encoded a 315-residue polypeptide consisting of a putative signal peptide of 18 residues and a catalytic domain belonging to glycosyl hydrolase family 16. The deduced amino acid sequence showed the highest identity (82%) with the putative endo-1,3(4)-β-glucanase from Talaromyces stipitatus ATCC 10500 and 60% identity with the characterized β-1,3(4)-glucanase from Paecilomyces sp. FLH30. The gene was successfully overexpressed in Pichia pastoris. Recombinant Bgl16C1 constituted 95% of total secreted proteins (2.61 g l⁻¹) with activity of 28,721 U ml⁻¹ in a 15-l fermentor. The purified recombinant Bgl16C1 had higher specific activity toward barley β-glucan (12,622 U mg⁻¹) than all known glucanases and also showed activity against lichenan and laminarin. The enzyme was optimally active at pH 5.0 and 55°C and exhibited good stability over a broad acid and alkaline pH range (>85% activity at pH 3.0-7.0 and even 30% at pH 11.0). All these favorable enzymatic properties make it attractive for potential applications in various industries.

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“…Alicyclobacillus sp. contain endoglucanases (Bai et al ., , b) and xylanases (Bai et al ., ; Wang et al ., ), as may some species of Sulfolobus (Girfoglio et al ., ). Most work has focused on fungal enzymes, some of these are quite acid‐ or thermal‐tolerant (Iefuji et al ., ; Kimura et al ., ; de Lemos Esteves et al ., ; Collins et al ., ; Viikari et al ., ; Zhang et al ., ), and include enzymes produced by taxa associated with geothermal or acid environments that we have found in BSL, such as Acidomyces or Phialophora .…”

Section: Discussionmentioning

confidence: 99%

Microbial heterotrophic production in an oligotrophic acidic geothermal lake: responses to organic amendments and terrestrial plant litter

Wolfe

Fitzhugh

Almasary

et al. 2014

FEMS Microbiol Ecol

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Boiling Springs Lake (BSL) is an oligotrophic, acidic geothermal feature where even very low levels of microbial heterotrophic production still exceed autotrophy. To test whether allochthonous leaf litter (LL) inputs fuel this excess, we quantified leaf litterfall, leaching and decomposition kinetics, and measured the impact of organic amendments on production, germination and cell growth, using pyrosequencing to track changes in microbial community composition. Coniferous leaves in BSL exhibited high mass loss rates during leaching and decomposition, likely due to a combination of chemical hydrolysis and contributions of both introduced and endemic microbes. We measured very low in situ (3)H-thymidine incorporation over hours by the dominant chemolithotroph Acidimicrobium (13-65 μg C L(-1) day(-1)), which was inhibited by simple C sources (acetate, glucose). Longer term incubations with additions of 0.01-0.02% complex C/N sources induced germination of the Firmicute Alicyclobacillus within 1-2 days, as well as growth of Acetobacteraceae after 3-4 days. LL additions yielded the opposite successional patterns of these r-selected heterotrophs, boosting production to 30-150 μg C L(-1) day(-1). Growth and germination studies suggest both prokaryotes and fungi likely consume allochthonous organics, and might be novel sources of lignocellulose-degrading enzymes. A model of BSL's C budget supports the hypothesis that allochthonous inputs fuel seasonal microbial heterotrophy, but that dissolved organic C sources greatly exceed direct LL inputs.

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A novel family 9 β-1,3(4)-glucanase from thermoacidophilic Alicyclobacillus sp. A4 with potential applications in the brewing industry

Cited by 43 publications

References 27 publications

Secretome analysis of Trichoderma reesei CICC41495 for degradation of arabinoxylan in malted barley

Secretome analysis of Trichoderma reesei CICC41495 for degradation of arabinoxylan in malted barley

High-level expression of a novel Penicillium endo-1,3(4)-β-d-glucanase with high specific activity in Pichia pastoris

Microbial heterotrophic production in an oligotrophic acidic geothermal lake: responses to organic amendments and terrestrial plant litter

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