A Novel ES Cell Line, TT2, with High Germline-Differentiating Potency

Yagi, Takeshi; Tokunaga, Tomoyuki; Furuta, Yasuhide; Nada, Shigeyuki; Yoshida, Michio; Tsukada, Takehiro; Saga, Yumiko; Takeda, Nobuo; Ikawa, Yoji; Aizawa, Shinichi

doi:10.1006/abio.1993.1458

Cited by 452 publications

(309 citation statements)

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“…The homologous recombinant ES clones and mutant mice were generated as described (Yagi et al, 1993;Murata et al, 2004). To determine the PCR condition to assign homologous recombinant ES clones, the control vector was electroporated into ES cells after SalI digestion.…”

Section: Isolation Of Homologous Recombinant Es Cells and Generation mentioning

confidence: 99%

“…The PCR conditions thus established were as follows: primers, p1(5Ј-CATCGCCTTCTATC-GCCTTCTTGACG-3Ј) and p2 (5Ј-TT-GGTTGTCACAGCCCTGCCTCTGC-3Ј) of which locations are indicated in Figure 3A; pretreatment, at 94°C for 1 min; 45 cycles of the PCR (denaturation, 20 sec at 96°C; annealing, 5 min at 68°C; extension, 15 min at 72°C). The targeting vector was linearized by the SalI digestion, and electroporated into TT2 ES cells (Yagi et al, 1993). Of 192 G418 resistant clones examined, 12 were assigned homologous recombinants by the PCR, 10 of which were confirmed to be correct homologous recombinants by Southern blot analysis (Fig.…”

Section: Isolation Of Homologous Recombinant Es Cells and Generation mentioning

confidence: 99%

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A new serine/threonine protein kinase,Omphk1, essential to ventral body wall formation

Hirano¹,

Kanazawa

Furushima

et al. 2006

Developmental Dynamics

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Here, we report a new serine/threonine protein kinase of the SNF1 subfamily Omphk1. Two Omphk homologues exist in each vertebrate species, and one homologue exists in Drosophila and Caenorhabditis elegans; the kinase domain is highly conserved among these homologues, and several domains are conserved among vertebrate Omphk. Omphk1 expression dynamically changes in the developing central nervous system, is found ubiquitously in epidermis, and is present uniquely in several other tissues. Its expression is also found in each tissue associated with the ventral body wall closure: the primary body wall composed of primitive ectoderm and each component of the secondary body wall. Concomitantly, its null mutant exhibits omphalocele with a failure in closure of the secondary body wall. There are no apparent gross morphological defects in brain, however, despite the unique Omphk1 expression in this tissue.

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Section: Isolation Of Homologous Recombinant Es Cells and Generation mentioning

confidence: 99%

Section: Isolation Of Homologous Recombinant Es Cells and Generation mentioning

confidence: 99%

A new serine/threonine protein kinase,Omphk1, essential to ventral body wall formation

Hirano¹,

Kanazawa

Furushima

et al. 2006

Developmental Dynamics

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“…A 6.0 kb XbaI fragment and a 0.5 kb HincII-HindIII fragment were blunted with T4 DNA polymerase, and inserted into PmeI and SmaI sites of the modified version of pND1, respectively. The resulting targeting vector was linearized with SacI, and introduced into TT2 ES cell lines as described previously (Yagi et al 1993a). Two out of 140 G418 resistant clones were isolated as positive clones by polymerase chain reaction (PCR) analysis using neomycin primer (5 0 -TCGTGCTTTACGC TATCGCCGCTCCCGATT-3 0 ) and p48 primer (5 0 -TCAATGTTCCGATGTGGCAGTTCAAAG-GATC-3 0 ).…”

Section: Disruption Of the P48 Gene In Mouse Es Cellsmentioning

confidence: 99%

Essential and non‐redundant roles of p48 (ISGF3γ) and IRF‐1 in both type I and type II interferon responses, as revealed by gene targeting studies

et al. 1996

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“…The genomic DNAs extracted from ES cells were digested with several enzymes, and Southern blot analyses were performed as described (Yagi et al 1993a,b;Matsuo et al 1995). Two mutant mouse lines were generated from two independent homologous recombinant ES cell clones (Yagi et al 1993a). Since no difference was found in the phenotypes between the two lines, no reference is made as to which lines were used in each analysis.…”

Section: Generation Of Mutant Micementioning

confidence: 99%

Otx1 function overlaps with Otx2 in development of mouse forebrain and midbrain

et al. 1996

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Background: We previously reported that the homozygous mutation of Otx2 gene, a mouse cognate of the Drosophila head gap gene orthodenticle, causes failure in the development of the rostral head anterior to rhombomere 3, which may correspond to earlier Otx2 expression in cells destined for the anterior mesoendoderm. At the same time, the Otx2 heterozygous mutation displayed a phenotype characterized as otocephaly, probably related to expression in the anterior neuroectoderm at the subsequent pharyngula stage. Defects were characteristic in the most anterior and posterior regions of Otx2 expression where Otx1, another mouse cognate of orthodenticle, is not or weakly expressed. They were not found in the region where Otx1 is expressed.

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A Novel ES Cell Line, TT2, with High Germline-Differentiating Potency

Cited by 452 publications

References 0 publications

A new serine/threonine protein kinase,Omphk1, essential to ventral body wall formation

A new serine/threonine protein kinase,Omphk1, essential to ventral body wall formation

Essential and non‐redundant roles of p48 (ISGF3γ) and IRF‐1 in both type I and type II interferon responses, as revealed by gene targeting studies

Otx1 function overlaps with Otx2 in development of mouse forebrain and midbrain

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