A Novel Equine Hoof Lamellar Tissue Biopsy Technique

Ribeiro, Rodrigo Martins; Mendes, Heloisa Maria Falcão; Valadares, R.C.; Paz, Cahuê Francisco Rosa; Oliveira, Alvaro de Paula Lage de; Spadeto, Odael; Faleiros, Rafael Resende

doi:10.1016/j.jevs.2016.10.007

Cited by 2 publications

(4 citation statements)

References 16 publications

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“…While the stereomicroscope or distance of the camera lens were not effective in determining configuration changes in the tissues and the limits between L-C and D-P, which were described in the qualitative findings of the transmural technique [1], histological sections could improve these aspects and confirm alterations to or normality of the hooves' soft tissues [19,20,[37][38][39]. The implementation of in vivo lamellar biopsy procedures with sedation techniques and regional anesthesia has also been described [38,39]. However, the invasive nature of the procedure could limit its applicability.…”

Section: Discussionmentioning

confidence: 99%

“…These observations are supported by the fact that the distance D was not statistically similar between ultrasonography and anatomical sections in Tables 1 and 2. Therefore, the implementation of histological sections in future studies would improve these aspects and confirm alterations to or the normality of the hooves soft tissues [19,20,[37][38][39]. However, the invasive nature of the in vivo lamellar biopsy procedure [38,39] could limit its applicability.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the implementation of histological sections in future studies would improve these aspects and confirm alterations to or the normality of the hooves soft tissues [19,20,[37][38][39]. However, the invasive nature of the in vivo lamellar biopsy procedure [38,39] could limit its applicability. The measurement of distance B, involving the tubular hoof wall (P), showed consistency between the methods used.…”

Section: Discussionmentioning

confidence: 99%

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Transmural Ultrasonography in the Evaluation of Horse Hoof Internal Structures: Comparative Quantitative Findings—Part 2

Castro-Mesa,

Resende Faleiros,

Martínez-Aranzales

2023

Animals

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The transmural ultrasound allows for the definition of the normal appearance of the hoof tissues and internal structures. Determining such measurements and proportions could contribute to evaluations of the normal spatial distal phalanx–hoof relationship. The objective was to describe the hoof’s dermis and epidermis measurements using the transmural technique, in comparison with DR and anatomical sections. Sixty-two healthy hooves without digital radiographic abnormalities, made up of 30 anatomical pieces (phase 1) and 32 alive horse hooves (phase 2), and 16 sagittal sections of the first ones were used. The proximal and distal planes were compared, defining the following measurements: coronary band-extensor process, distal phalanx apex–hoof wall, sole dermis, middle stratum, parietal dermis, lamellar layer, the sublamellar dermis, and ratios between them. Most of the measurements were consistent among methods. Some showed measurement differences and a minority was impossible to determine. The transmural technique allowed for the observation and replication of measurements of the hoof structures, described with other diagnostic aids. It also allowed for the implementation of new measurements that would help to determine the spatial location of the distal phalanx. Additionally, it contributes to the visualization of normal findings, which will be useful to compare variations in the different phases of laminitis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Transmural Ultrasonography in the Evaluation of Horse Hoof Internal Structures: Comparative Quantitative Findings—Part 2

Castro-Mesa,

Resende Faleiros,

Martínez-Aranzales

2023

Animals

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“…Aseptic preparation of the dorsal hoof wall was performed using 2% chlorhexidine and alcohol 70%. Lamellar biopsies were obtained from the dorsal portion of hoof capsule [ 19 ], and the samples were placed in 10% buffered formalin. Subsequently, the biopsy site was covered with dental impression material (Perfil Coltene, Brazil) soaked in 10% gentamicin, followed by application of a compressive bandage.…”

Section: Methodsmentioning

confidence: 99%

Histological comparison of the lamellar tissue of Iberian origin breed horses created in semi-feral conditions or in an intensive system

et al. 2023

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Although the external conformation of wild horse hooves has been proposed as an ideal model for domesticated modern horses, histological signs of laminitis have been reported among them. With the hypothesis that the lamellar tissue of horses of Iberian origin raised in semi-feral is healthier than those raised in an intensive management system (stall confinement and high-calorie diet intake), the objective was to compare their lamellar tissues. Lamellar tissue samples were taken from the forelimb hoof of eight domesticated Mangalarga Marchador (MM) horses and from six semi-feral Marajoara (MJ) and Puruca (MP) horses. Primary epidermal lamella (PEL) and secondary epidermal lamella (SEL) were measured (length and width) in several regions, and their shapes were morphologically classified into different types. Breed groups were compared using analysis of variance, followed by Tukey or Dunn tests (P<0.05). Early signs of laminitis such as abnormal keratinization of the abaxial primary dermal lamella, tapered tips of the axial PELs, pointed tips of the SEL, nuclei condensation, and abnormal spatial orientation of the secondary epidermal basal cells (SELBC), were seen only in MM horses. MP horses had a greater interface of epidermis/dermis contact than MJ horses and more rounded nuclei in the round SELBC than MM horses. In agreement with the study hypothesis, semi-feral MJ and MP horses’ lamellae were classified as healthier than MM horses, which showed early signs of endocrinopathic laminitis.

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A Novel Equine Hoof Lamellar Tissue Biopsy Technique

Cited by 2 publications

References 16 publications

Transmural Ultrasonography in the Evaluation of Horse Hoof Internal Structures: Comparative Quantitative Findings—Part 2

Transmural Ultrasonography in the Evaluation of Horse Hoof Internal Structures: Comparative Quantitative Findings—Part 2

Histological comparison of the lamellar tissue of Iberian origin breed horses created in semi-feral conditions or in an intensive system

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