A Novel Enzyme Immunoassay for Conidia of <i>Fusarium oxysporum</i> f.sp. <i>cucumerinum</i> F504

Kitagawa, Tsunehiro; Sakamoto, Yuichiro; Ogura, Hirosuke

doi:10.1111/j.1348-0421.1989.tb00963.x

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…In contrast, the assay with Ssp was significantly less sensitive than with Smy when tested against mycelial extracts. These latter results are in accordance with those of Kitagawa, Sakamoto & Ogura (1989) who had produced an anticonidium serum against a Fusarium oxysporum strain that almost did not react with the mycelium. This may be due to differences of immunogen concentration.…”

Section: Discussionsupporting

confidence: 91%

Comparison of responses of ascospores and mycelium by ELISA with anti‐mycelium and anti‐ascospore antisera for the development of a method to detect Sclerotinia sclerotiorum on petals of oilseed rape

Jamaux-Despréaux¹,

Spire²

1999

Annals of Applied Biology

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The goal of this project was the development of a serological test for the detection of Sclerotinia sclerotiorum on oilseed rape petals. Since the fungus exists in two forms on petals, as ascospores and mycelium, the responses of anti-mycelium and anti-ascospore antisera against these two kinds of antigens were compared. Two anti-mycelium sera, Smy and Smy', were produced against mycelial soluble extracts at different concentrations (0.3 mg and 0.1 mg of protein ml-I). Smy gave the greatest response level with eight S. sclerotiorum mycelial extracts tested. It had a very superior level of recognition to that of anti-ascospore serum, Ssp, when mycelium was tested as antigen. In contrast, Ssp and Smy were equally reactive when exposed to ascospores but the sensitivity of the assay was low. For each antiserum, the solution from which ascospores had been removed reacted similarly to the original suspension containing ascospores. A collection of fungi likely to be found on oilseed rape petals was examined. Cross-reactions were produced with both antisera, especially with Botrytis cinerea for which greater cross-reactivities were produced with Smy. The cross-absorption of antiserum Smy with a mycelial extract of B. cinerea considerably reduced this cross-reaction. The choice of antiserum for the development of a reliable detection system is discussed.

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Section: Discussionsupporting

confidence: 91%