A novel enzymatic decarboxylation of oxalic acid by the lignin peroxidase system of white‐rot fungus <i>Phanerochaete chrysosporium</i>

Akamatsu, Yasumi; Ma, Dengbo; Higuchi, Takayoshi; Shimada, Morimi

doi:10.1016/0014-5793(90)81169-o

Cited by 68 publications

(49 citation statements)

References 15 publications

(4 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, ODH [3], as another key enzyme of the TCA cycle, was not detected, and NAD-specific IDH also was not found (data not shown). Thus, it has been shown that this fungus has the modified TCA cycle with ICL [1] as a major enzyme and does not contain the Krebs TCA cycle, which oxidizes 1 mol acetyl-CoA to 2 mol CO 2 (dotted line in Fig.…”

Section: Resultsmentioning

confidence: 95%

“…Fig. 3 A and B show the changes in total and specific activities, respectively, of the two branch-point enzymes (ICL and IDH) and ODH [3]. Interestingly, the results Glyoxylate dehydrogenase (GLOXDH) 65 *The enzymes, listed in numerical order, correspond to enzymes that catalyze the reaction numbered in the metabolic system in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…5 indicates that 2 mol acetyl-CoA, derived from 1 mol glucose through the glycolysis shunt, enter Cycles A and B first and finally exit as oxalate of the end product from recycling routes C and D. A biochemical role of the metabolic system is to primarily oxidize acetyl-CoA to yield oxalate, which accumulates in the culture fluid, because this fungus does not contain oxalate-decomposing enzyme systems such as oxalate oxidase (42), oxalate decarboxylase (43,44), and the ligninolytic systems occurring in white-rot fungi (3)(4)(5)(6)(7).…”

Section: Biochemical Role Of a Metabolic System For Biosynthesis Of Omentioning

confidence: 99%

See 2 more Smart Citations

A physiological role for oxalic acid biosynthesis in the wood-rotting basidiomycete Fomitopsis palustris

Munir

Yoon

Tokimatsu

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

147

112

View full text Add to dashboard Cite

A metabolic mechanism for oxalic acid biosynthesis in the woodrotting basidiomycete Fomitopsis palustris has been proposed on the basis of biochemical analyses of glucose metabolism. There was a strong correlation between glucose consumption and oxalate production. Oxalic acid was found to accumulate in the culture fluid in about 80% of the theoretical yield or about 5-fold, on the basis of the fungal biomass harvested. The results clearly indicate that glucose was not completely oxidized to CO2 by the tricarboxylic acid (TCA) cycle but converted mainly to oxalate. The determination of the 12 enzymes concerned has revealed the occurrence of the unprecedented metabolic coupling of the TCA and glyoxylate cycles that support oxalate biosynthesis. In this metabolic system, isocitrate lyase (EC 4.1.3.1), together with oxaloacetase (EC 3.7.1.1), was found to play a pivotal role in yielding oxalate from oxaloacetate via the acetate-recycling routes. Moreover, malate dehydrogenase (EC 1.1.1.37), with an extraordinarily high activity among the enzymes tested, was shown to play an important role in generating NADH by oxidation of malate to oxaloacetate. Thus, it is proposed that the wood-rotting basidiomycete acquires biochemical energy by oxidizing glucose to oxalate.

show abstract

Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Section: Biochemical Role Of a Metabolic System For Biosynthesis Of Omentioning

confidence: 99%

See 1 more Smart Citation

A physiological role for oxalic acid biosynthesis in the wood-rotting basidiomycete Fomitopsis palustris

Munir

Yoon

Tokimatsu

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

147

112

View full text Add to dashboard Cite

show abstract

“…It has been suggested as a detoxification mechanism for excess cations such as copper (Murphy and Levy 1983;Sutter et al 1983), and involved in cellulose depolymerisation (Espejo and Agos/n 1991;Schmidt et al 1981). Recent research on the whiterot fungus, Phanerochaete chrysosporium has suggested that oxalate might play a role in lignin depolymerisation (Popp et al 1990;Akamatsu et al 1990).…”

Section: Introductionmentioning

confidence: 99%

Oxalate production by Basidiomycetes, including the white-rot species Coriolus versicolor and Phanerochaete chrysosporium

Dutton¹,

Evans²,

Atkey³

et al. 1993

Appl Microbiol Biotechnol

155

View full text Add to dashboard Cite

Oxalate was found to accumulate in liquid culture media from the growth of the white-rot basidiomycetes Coriolus versicolor, Heterobasidion annosum, Pleurotus florida and Phanerochaete chrysosporium. Whereas little oxalate accumulated during active growth, millimolar concentrations of oxalate were detected in culture media during the stationary phase. The basidiomycete Agaricus bisporus, the cultivated mushroom, also accumulated oxalate in its culture medium in the stationary phase. In comparison, the brown-rot fungi Amyloporia xantha, Coniophora marmorata, C. puteana and Poria vaporaria accumulated oxalate in the primary metabolic phase and throughout growth up to 35 days. Oxalate accumulation (0.04-10.0 mM) in whiterot cultures did not lower the pH of the medium during growth, whereas in brown-rot cultures oxalate (2.0-20.0 mM) reduced the media pH during growth. Cultures of Agaricus bisporus, C. puteana and Coriolus versicolor grown on solid media containing high levels of calcium (50 or 100 mM calcium chloride) produced calcium oxalate crystals to varying extents on the surface of the hyphae.

show abstract

“…We demonstrated that this reaction involves the decarboxylation of EDTA by the veratryl alcohol cation radical (15). There was also a report in the literature that the fungus produces oxalate and that it is decarboxylated by lignin peroxidase in the presence of veratryl alcohol (17). The mechanism by which veratryl alcohol and oxalate can catalyze reduction is shown in Figure 2.…”

mentioning

confidence: 88%

Mechanisms of Degradation by White Rot Fungi

Aust¹

1995

Environmental Health Perspectives

View full text Add to dashboard Cite

White rot fungi use a variety of mechanisms to accomplish the complete degradation of lignin and a wide variety of environmental pollutants. Both oxidative and reductive reactions are required for the metabolism of both lignin and environmental pollutants. The fungi secrete a family of peroxidases to catalyze both direct and indirect oxidation of chemicals. The peroxidases can also catalyze reductions using electron donors to generate reductive radicals. A cell-surface membrane potential can also be used to reduce chemicals such as TNT. -Environ Health Perspect 103(Suppl 5):59-61 (1995)

show abstract

A novel enzymatic decarboxylation of oxalic acid by the lignin peroxidase system of white‐rot fungus Phanerochaete chrysosporium

Cited by 68 publications

References 15 publications

A physiological role for oxalic acid biosynthesis in the wood-rotting basidiomycete Fomitopsis palustris

A physiological role for oxalic acid biosynthesis in the wood-rotting basidiomycete Fomitopsis palustris

Oxalate production by Basidiomycetes, including the white-rot species Coriolus versicolor and Phanerochaete chrysosporium

Mechanisms of Degradation by White Rot Fungi

Contact Info

Product

Resources

About