A novel electrochemical PCB77-binding DNA aptamer biosensor for selective detection of PCB77

Wu, Lidong; Qi, Peipei; Fu, Xiaochen; Liu, Huan; Li, Jincheng; Wang, Qiong; Fan, Hao

doi:10.1016/j.jelechem.2016.04.003

Cited by 32 publications

(22 citation statements)

References 36 publications

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“… 36 Further, the achieved detection limit is 3 orders superior to most of the previous reports as shown in Table 1 . 5 , 13 , 18 , 37 − 39 Noted elsewhere, 40 a single Au nanoneedle (400 nm in diameter) modulated by β-cyclodextrin was proposed as the electrode to realize a detection limit of 0.21 fM, while its linear detection was in a narrow range of 2–16 fM. Comparatively, the ATA/BDD sensor offers a rigid and recyclable diamond-based electrode with a wide linearity range and low background noise signals, implying that the electrode in this work is more favorable for practical applications.…”

Section: Results and Discussionmentioning

confidence: 99%

“… 15 − 17 It was reported that the aptamers added in carbon nanotubes could importantly increase the detection limit to nM. 18 Also, Au-based biosensors with aptamers could realize the detection limit to pM 13 since Au electrodes had the advantages of high electrical conductivity, chemical stability, and easy attachment to aptamers. 19 , 20 Au-nanoparticles (Au-NPs) with large specific surface area would provide more adsorption sites for aptamers by forming Au–S bonds.…”

Section: Introductionmentioning

confidence: 99%

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Polychlorinated Biphenyl Electrochemical Aptasensor Based on a Diamond–Gold Nanocomposite to Realize a Sub-Femtomolar Detection Limit

et al. 2020

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Polychlorinated biphenyls (PCBs) with high toxicity, low lethal dose, and bioaccumulation have been inhibited for application in wide fields, and a highly efficient trace detection is thus greatly desirable. In this study, we produce dense Au-nanoparticles by twice sputtering and twice annealing (T-Au-NPs) on boron-doped diamond (BDD). The successful formation of T-Au-NPs/BDD nanocomposites was confirmed by scanning electron microscopy, X-ray diffraction, Raman spectroscopy, and X-ray photoelectron spectroscopy analysis. Based on T-Au-NPs/BDD, an electronic biosensor with aptamers is fabricated to detect trace polychlorinated biphenyl-77 (PCB-77) by electrochemical impedance. A good linear relationship in the range of femtomolar to micromolar and significantly low detection limit of sub-femtomolar level (0.32 fM) are realized based on the biosensor. The emphasis of this research lies in the key role of the diamond substrate in the biosensor. It is demonstrated that the biosensor has excellent sensitivity, specificity, stability, and recyclability, which are favorable for detecting the trace PCB-77 molecule. It is attributed to the important effect presented by the BDD substrate and the synergistic influence of T-Au-NPs combined with aptamers.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polychlorinated Biphenyl Electrochemical Aptasensor Based on a Diamond–Gold Nanocomposite to Realize a Sub-Femtomolar Detection Limit

et al. 2020

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“…Aptamers had been used extensively in drug transfer, protein analysis, immunodiagnosis and treatment, biosensors, due to their many unique properties, such as easy synthesis, excellent affinity, high specificity, nice stability, and simple modification. A variety of novel technologies and programs based aptamers have been studied, including microfluidics [ 61 ], fluorescent [ 62 ], and electrochemical processes [ 63 ].…”

Section: Aptamer Biosensor Based On Rcamentioning

confidence: 99%

“…In contrast, aptamers can be synthesized in vitro through the SELEX technique. Aptamers have been widely used to fabricate biosensors in numerous new fields of study [ 63 , 103 , 104 , 105 ].…”

Section: Protein Biosensor Based On Rcamentioning

confidence: 99%

Research Progress on Rolling Circle Amplification (RCA)-Based Biomedical Sensing

Yan

Liu

et al. 2018

Pharmaceuticals

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Enhancing the limit of detection (LOD) is significant for crucial diseases. Cancer development could take more than 10 years, from one mutant cell to a visible tumor. Early diagnosis facilitates more effective treatment and leads to higher survival rate for cancer patients. Rolling circle amplification (RCA) is a simple and efficient isothermal enzymatic process that utilizes nuclease to generate long single stranded DNA (ssDNA) or RNA. The functional nucleic acid unit (aptamer, DNAzyme) could be replicated hundreds of times in a short period, and a lower LOD could be achieved if those units are combined with an enzymatic reaction, Surface Plasmon Resonance, electrochemical, or fluorescence detection, and other different kinds of biosensor. Multifarious RCA-based platforms have been developed to detect a variety of targets including DNA, RNA, SNP, proteins, pathogens, cytokines, micromolecules, and diseased cells. In this review, improvements in using the RCA technique for medical biosensors and biomedical applications were summarized and future trends in related research fields described.

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“…Among them, label-free electrochemical impedance spectroscopy (EIS) as a powerful and sensitive technique to monitor aptamer-ligand binding has appeared as a promising strategy [ 56 ]. More importantly, EIS method is nondestructive, which makes it highly attractive for aptamer-based low weight molecules detection [ 57 ]. In addition, some novel methods using microwires formed by platinum nanoparticles (PtNPs) [ 58 ], AuNPs dotted rGO nanocomposite [ 59 ], thionine–graphene nanocomposites [ 60 ] are developed for detecting low molecular weight pollutants in water samples recently.…”

Section: Aptasensors and Their Applications For Detecting Low Molementioning

confidence: 99%

Practical Application of Aptamer-Based Biosensors in Detection of Low Molecular Weight Pollutants in Water Sources

et al. 2018

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Water pollution has become one of the leading causes of human health problems. Low molecular weight pollutants, even at trace concentrations in water sources, have aroused global attention due to their toxicity after long-time exposure. There is an increased demand for appropriate methods to detect these pollutants in aquatic systems. Aptamers, single-stranded DNA or RNA, have high affinity and specificity to each of their target molecule, similar to antigen-antibody interaction. Aptamers can be selected using a method called Systematic Evolution of Ligands by EXponential enrichment (SELEX). Recent years we have witnessed great progress in developing aptamer selection and aptamer-based sensors for low molecular weight pollutants in water sources, such as tap water, seawater, lake water, river water, as well as wastewater and its effluents. This review provides an overview of aptamer-based methods as a novel approach for detecting low molecular weight pollutants in water sources.

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A novel electrochemical PCB77-binding DNA aptamer biosensor for selective detection of PCB77

Cited by 32 publications

References 36 publications

Polychlorinated Biphenyl Electrochemical Aptasensor Based on a Diamond–Gold Nanocomposite to Realize a Sub-Femtomolar Detection Limit

Polychlorinated Biphenyl Electrochemical Aptasensor Based on a Diamond–Gold Nanocomposite to Realize a Sub-Femtomolar Detection Limit

Research Progress on Rolling Circle Amplification (RCA)-Based Biomedical Sensing

Practical Application of Aptamer-Based Biosensors in Detection of Low Molecular Weight Pollutants in Water Sources

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