A novel electrochemical enzyme biosensor for detection of 17β-estradiol by mediated electron-transfer system

Wang, Andrew; Ding, Yaping; Li, Li; Duan, Dingding; Mei, Qianwen; Qi, Zhuang; Cui, Shiqiang; He, Xinyu

doi:10.1016/j.talanta.2018.09.018

Cited by 52 publications

(20 citation statements)

References 33 publications

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“…E2 has been used frequently in animal husbandry to promote animal growth, increasing the lean meat percentage and milk production. The excess E2 may remain in food like meat, milk and may cause women‘s fertility problem and increasing the risk of exposure to ovarian and breast cancer [5]. In some countries, there are residual E2 limits, which have been stipulated for products such as meat, but there is no regulation for dairy product [6].…”

Section: Introductionmentioning

confidence: 99%

Sensitive Determination of 17β‐Estradiol using a Magneto Sensor Based on Magnetic Molecularly Imprinted Polymer

et al. 2020

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The analysis of 17β‐estradiol with high sensitivity and selectivity is extremely relevant to control the impacts that this compound can cause on health and the environment. Thus, we describe the development and application of a magneto carbon paste electrode based on magnetic molecularly imprinted polymer (MCPE‐MMIP) for determination of 17β‐estradiol. The analyte adsorbed on the MMIP was immobilized on the electrode surface by magnetic capture. The morphological and structural characterization of the obtained MMIP suggests that the material was effectively synthesized. MCPE‐MMIP showed an improvement in the sensitivity for 17β‐estradiol detection when compared to electrode configurations in the absence of this material. The optimum conditions (0.10 mol L−1 phosphate buffer pH 7.0) were reached by differential pulse adsorptive stripping voltammetry (DPAdSV), in which the method presented linearity ranged from 0.06 to 175 μmol L−1 with limits of detection and quantification of 0.02 and 0.06 μmol L−1, respectively. The proposed sensor was applied effectively in the analysis of 17β‐estradiol in river water and raw milk samples, exhibiting excellent recovery values (between 96.20 and 104 %), which were confirmed by HPLC analysis.

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Section: Introductionmentioning

confidence: 99%

Sensitive Determination of 17β‐Estradiol using a Magneto Sensor Based on Magnetic Molecularly Imprinted Polymer

et al. 2020

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“…The responses obtained with ten different dual immune platforms prepared in the same way in the same day for 1 ng mL −1 E2 provided a relative standard deviation (RSD) value of 2.7 %, which proves the good reproducibility of the whole working protocol. It is worth highlighting the simplicity and the good sensitivity achieved with the immune platform and in a test time considerably shorter than that required by previously reported for the preparation of electrochemical enzymatic biosensors (∼6 [21] and 48 [22] h). In addition, compared with the available ELISA kits, the immune platform allows working in a decentralized and multiplexed way [23, 24].…”

Section: Resultsmentioning

confidence: 93%

Multiplexed Determination of Fertility‐related Hormones in Saliva Using Amperometric Immunosensing

et al. 2021

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This paper reports a dual immunosensor for the simultaneous determination of two important fertility‐related hormones: 17β‐estradiol, E2, and follicle‐stimulating hormone, FSH. The implemented method involves direct competitive (E2) or sandwich‐type (FSH) immunoassays carried out on magnetic microparticles (MBs) and amperometric detection at screen‐printed dual carbon electrodes (SPdCEs) involving the hydroquinone (HQ)/H2O2 system. The developed immune platform demonstrates LOD values of 6.88 pg mL−1 and 0.11 mIU mL−1 for E2 and FSH standards, respectively and usefulness for the determination in saliva samples collected from different volunteers, giving results in agreement with the conventional ELISA methodologies.

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“…E2 and other natural and synthetic compounds can mimic endogenous hormones [220]. They can interfere with the proper functioning of the hormonal, immune, and nervous systems of mammals [221]. Zhang et al fabricated a polymeric/enzymatic biosensor for sensing E2 with electrodeposited poly (4,7-bis(5-(3,4-ethylenedioxythiophene)thiophen-2-yl)benzothiadiazole) (Pol) and HRP onto platinum electrode [222].…”

Section: -β-Estradiol (E2)mentioning

confidence: 99%

Electrochemical Sensors Based on Conducting Polymers for the Aqueous Detection of Biologically Relevant Molecules

et al. 2021

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Electrochemical sensors appear as low-cost, rapid, easy to use, and in situ devices for determination of diverse analytes in a liquid solution. In that context, conducting polymers are much-explored sensor building materials because of their semiconductivity, structural versatility, multiple synthetic pathways, and stability in environmental conditions. In this state-of-the-art review, synthetic processes, morphological characterization, and nanostructure formation are analyzed for relevant literature about electrochemical sensors based on conducting polymers for the determination of molecules that (i) have a fundamental role in the human body function regulation, and (ii) are considered as water emergent pollutants. Special focus is put on the different types of micro- and nanostructures generated for the polymer itself or the combination with different materials in a composite, and how the rough morphology of the conducting polymers based electrochemical sensors affect their limit of detection. Polypyrroles, polyanilines, and polythiophenes appear as the most recurrent conducting polymers for the construction of electrochemical sensors. These conducting polymers are usually built starting from bifunctional precursor monomers resulting in linear and branched polymer structures; however, opportunities for sensitivity enhancement in electrochemical sensors have been recently reported by using conjugated microporous polymers synthesized from multifunctional monomers.

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A novel electrochemical enzyme biosensor for detection of 17β-estradiol by mediated electron-transfer system

Cited by 52 publications

References 33 publications

Sensitive Determination of 17β‐Estradiol using a Magneto Sensor Based on Magnetic Molecularly Imprinted Polymer

Sensitive Determination of 17β‐Estradiol using a Magneto Sensor Based on Magnetic Molecularly Imprinted Polymer

Multiplexed Determination of Fertility‐related Hormones in Saliva Using Amperometric Immunosensing

Electrochemical Sensors Based on Conducting Polymers for the Aqueous Detection of Biologically Relevant Molecules

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