A novel electrochemical biosensor based on Fe 3 O 4 nanoparticles-polyvinyl alcohol composite for sensitive detection of glucose

Abstract

In this research, a new electrochemical biosensor was constructed for the glucose detection. Iron oxide nanoparticles (FeO) were synthesized through co-precipitation method. Polyvinyl alcohol-FeO nanocomposite was prepared by dispersing synthesized nanoparticles in the polyvinyl alcohol (PVA) solution. Glucose oxidase (GOx) was immobilized on the PVA-FeO nanocomposite via physical adsorption. The mixture of PVA, FeO nanoparticles and GOx was drop cast on a tin (Sn) electrode surface (GOx/PVA-FeO/Sn). The FeO nanoparticles were characterized by X-ray diffraction (XRD). Also, Fourier transform infrared (FTIR) spectroscopy and field emission scanning electron microscopy (FE-SEM) techniques were utilized to evaluate the PVA-FeO and GOx/PVA-FeO nanocomposites. The electrochemical performance of the modified biosensor was investigated using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Presence of FeO nanoparticles in the PVA matrix enhanced the electron transfer between enzyme and electrode surface and the immobilized GOx showed excellent catalytic characteristic toward glucose. The GOx/PVA-FeO/Sn bioelectrode could measure glucose in the range from 5 × 10 to 30 mM with a sensitivity of 9.36 μA mM and exhibited a lower detection limit of 8 μM at a signal-to-noise ratio of 3. The value of Michaelis-Menten constant (K) was calculated as 1.42 mM. The modified biosensor also has good anti-interfering ability during the glucose detection, fast response (10 s), good reproducibility and satisfactory stability. Finally, the results demonstrated that the GOx/PVA-FeO/Sn bioelectrode is promising in biosensor construction.

Carbon nanofiber modified with osmium based redox polymer for glucose sensing

“…Here obtained value of the Michaelis-Menten constant of 0.99 mM is much lower than 5.20 mM for GOX/gold-platinum alloy nanoparticles/carbon nanotubes/chitosan system [41] and also 1.42 mM for GOX/PVA-Fe3O4/Sn [42]. Our experiment was performed in the acetate buffer of pH 5.5 which is slightly different from the optimum pH for GOX activity.…”

Disposable Amperometric Biosensor Based on Poly‐L‐lysine and Fe3O4 NPs‐chitosan Composite for the Detection of Tyramine in Cheese

“…The high conductivity of PLL/SPCE could be ascribed to the electrostatic attraction between Fe(CN) 6 3−/4− and positively charged PLL molecules due to its plentiful active amino groups . In addition, the decrease in electron transfer resistance of Fe 3 O 4 NP−CH/PLL/SPCE may be attributed to the improved electron transfer rate accelerated by the movement of electrons through hydrogen bonds between CH and PLL and the presence of highly conductive Fe 3 O 4 NP on the electrode surface .…”

“…The CVs of (a)PLL/SPCE, (b)Fe 3 O 4 −CH/SPCE, (c)Fe 3 O 4 −CH/PLL/SPCE and (d)Ty/Fe 3 O 4 −CH/PLL/SPCE obtained in 100 mM KCl solution containing 5.0 mM Fe(CN) 6 3−/4− are shown in Figure A. The Fe 3 O 4 −CH/SPCE exhibited higher currents than PLL/SPCE which may be assigned to the existence of Fe 3 O 4 NP with high electron mobility at the electrode surface resulting in enhanced electron transfer . After the PLL/SPCE was modified with Fe 3 O 4 −CH composite the peak currents were observed to be higher than those obtained with PLL/SPCE and Fe 3 O 4 −CH/SPCE.…”

“…Fe 3 O 4 NP have been also considered as useful materials for biomolecule immobilization and they can provide a suitable microenvironment for biomolecules to exchange electrons directly with an electrode, resulting in improved sensitivity of electrochemical biosensing platforms . Different authors have pointed out that the aggregation and rapid degradation drawbacks of Fe 3 O 4 NP can be eliminated by modifying them, using conductive polymers and biopolymers .…”

“…To date, various polymer and Fe 3 O 4 NP composites such as polypyrrole/Fe 3 O 4 NP , Fe 3 O 4 NP/chitosan‐g‐polyaniline , Fe 3 O 4 NP‐polyvinyl alcohol have been investigated. The main goal of these approaches is to improve the sensing capabilities of sensor and biosensors, such as sensitivity, operational stability, reproducibility, selectivity and long‐term stability.…”