A Novel Early Estrogen-Regulated Gene gec1 Encodes a Protein Related to GABARAP

Vernier-Magnin, Sandrine; Müller, Stephanie; Sallot, Myriam; Radom, Jean; Musard, Jean-François; Adami, Pascale; Dulieu, Philippe; Rémy-Martin, Jean-Paul; Jouvenot, Michèle; Fraîchard, Annick

doi:10.1006/bbrc.2001.4908

Cited by 30 publications

(20 citation statements)

References 30 publications

(29 reference statements)

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“…The highest expression levels were initially observed in the brain, heart, liver, skeletal muscle, kidney, spleen, ovary, small intestine, placenta and peripheral blood leukocytes. 4,7,19 In contrast to its differential expression in adult human tissues, GABARAPL1 is present at comparable mRNA levels in all fetal tissues.…”

Section: The Gabarap Familymentioning

confidence: 95%

“…It is also worth mentioning that the GABARAPL1 protein sequence is highly conserved throughout evolution from plants to mammals (100% identity) suggesting that this protein plays an essential role in these organisms. 4,7 Regulation and Expression of the GABARAPL1 Gene GABARAPL1 mRNA is expressed in a variety of tissues within the mouse, rat and human. The highest expression levels were initially observed in the brain, heart, liver, skeletal muscle, kidney, spleen, ovary, small intestine, placenta and peripheral blood leukocytes.…”

Section: The Gabarap Familymentioning

confidence: 99%

“…in the guinea pig gene, the iNr element is sufficient to direct transcription and the ere located in the first exon permits induction of the gene by estradiol-17β via erα. 4 These sequences are conserved in the human gene but their roles have not yet been addressed. A similar analysis of the equivalent region for the GABARAP gene revealed no estrogen responsive cis-regulatory sequences.…”

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GABARAPL1 (GEC1): Original or copycat?

Grand

Chakrama²,

Seguin-Py³

et al. 2011

Autophagy

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Section: The Gabarap Familymentioning

confidence: 95%

Section: The Gabarap Familymentioning

confidence: 99%

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GABARAPL1 (GEC1): Original or copycat?

Grand

Chakrama²,

Seguin-Py³

et al. 2011

Autophagy

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“…[9][10][11] GABARAPL1 was first discovered in 1993 and described as part of the GABARAP family in 2001. 12 When performing a far from exhaustive search on PubMed, we counted about 31 publications citing this protein (using three different names: GEC1, GABARAPL1 or GABARAP-L1). Within these 31 publications, only nine presented antibody-detection of the GABARAPL1 protein.…”

Section: Introductionmentioning

confidence: 99%

GABARAPL1 antibodies: Target one protein, get one free!

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Chakrama

Seguin-Py³

et al. 2011

Autophagy

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“…GABARAP interacts with the ␥2 subunit of the GABA A receptor (15), promotes clustering of the GABA A receptor, modulates the binding affinity of GABA, and alters the kinetics of desensitization and inactivation of the receptor (23). To date no biological functions have been demonstrated for GEC1.Following cloning of the guinea pig GEC1 (1), GEC1 has been cloned from several species (13,24). The amino acid sequences are identical * This work was supported by National Institutes of Health Grants DA17302 and DA04745.…”

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GEC1 Interacts with the κ Opioid Receptor and Enhances Expression of the Receptor

Chen

et al. 2006

Journal of Biological Chemistry

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We identified a truncated form (38 -117) of GEC1 that interacts with the C-tail of the human opioid receptor (hKOR) by yeast two-hybrid screening. GEC1-(38 -117) did not interact with the C-tail of the or ␦ opioid receptors. GEC1, a 117-amino acid protein (Pellerin, I., Vuillermoz, C., Jouvenot, M., Ordener, C., Royez, M., and Adessi, G. L. (1993) Mol. Cell Endocrinol. 90, R17-R21), is highly homologous to GABARAP, GATE-16, and Apg8/aut7, all members of the microtubule associated protein (MAP) family. In pull-down assays, GST-GEC1 interacted directly with the hKOR C-tail, full-length hKOR, and tubulin. When expressed in Chinese hamster ovary (CHO) cells, GEC1 co-immunoprecipitated with FLAG-hKOR. Expression of GEC1 greatly increased total and cellsurface KOR but not or ␦ opioid receptors. GEC1 expression slightly reduced U50,488H-promoted down-regulation, without affecting ligand binding affinity, receptor-G protein coupling, or U50,488H-induced desensitization and internalization. HA-GEC1 expressed in CHO cells was localized in the Golgi apparatus and endoplasmic reticulum (ER). When cells were pulsed with [ 35 S]Met/ Cys, GEC1 expression enhanced the level of the mature form (55-kDa band) of FLAG-hKOR at 4, 8, and 22 h after pulse without affecting the precursors (39-and 45-kDa bands), indicating that GEC1 facilitates trafficking of FLAG-hKOR from the ER/Golgi to plasma membranes. GEC1 interacted with N-ethylmaleimide-sensitive factor (NSF) in pull-down assays and co-immunoprecipitated with NSF in rat brain extracts. The interaction with NSF may contribute to GEC1 effects. This is the first report on biological functions of GEC1 and the first demonstration that a GPCR interacts with a protein of the MAP family. The interaction is important for trafficking of the receptor in the biosynthesis pathway.Activation of opioid receptors in vivo produces many effects, including antinociception (1, 2), psychotomimesis (2, 3), and water diuresis (1, 2). At the cellular level, opioid receptors are coupled through pertussis toxin-sensitive G proteins to affect a variety of effectors, which include adenylate cyclase, potassium, and calcium channel and the p42/ p44 mitogen-activated protein kinase (for a review, see Ref. 5). Following cloning of the ␦ opioid receptor, the opioid receptor has been cloned from several species (for a review, see Ref. 6). The opioid receptors belong to the rhodopsin sub-family of the G protein-coupled receptor (GPCR) 2 family.In recent years, GPCRs have been found to interact with many proteins besides G proteins, and such non-G protein interactions are unique to individual receptors and have been shown to play significant roles in signal transduction, receptor regulation, or receptor biogenesis (for reviews, see Refs. 7-9). We have demonstrated that activation of opioid receptors stimulates Na ϩ , H ϩ -exchanger 3 activity via NHERF-1/EBP-50 independent of pertussis toxin-sensitive G proteins (10). In this study, we identified proteins interacting with the human opioid receptor (hKOR) by yeast ...

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A Novel Early Estrogen-Regulated Gene gec1 Encodes a Protein Related to GABARAP

Cited by 30 publications

References 30 publications

GABARAPL1 (GEC1): Original or copycat?

GABARAPL1 (GEC1): Original or copycat?

GABARAPL1 antibodies: Target one protein, get one free!

GEC1 Interacts with the κ Opioid Receptor and Enhances Expression of the Receptor

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