A novel dual-color total internal reflection fluorescence detecting platform using compact optical structure and silicon-based photodetector

Song, Dan; Yang, Rong; Fang, Shunyan; Liu, Yanping; Liu, Jiayao; Xu, Wenjuan; Long, Feng; Zhu, Anna

doi:10.1016/j.talanta.2018.12.039

Cited by 9 publications

(6 citation statements)

References 25 publications

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“…The simultaneous detections of aflatoxin M1 (AFM1) and ochratoxin A (OTA) [ 54 ], Escherichia coli ( E. coli ) O157:H7 and Salmonella typhimurium [ 55 ], or acetamiprid and fipronil [ 56 ] were respectively achieved. The group further simplified the optical structure, where a single-multi mode fiber optic coupler was employed to replace the sophisticated confocal optical system for the transmission of two excitation lights and dual-color fluorescence [ 57 , 58 ]. A photodiode detector was used instead of a photomultiplier for the simultaneous detection of dual-color fluorescence.…”

Section: The Major Components Of Fluorescent Foew Sensors and Optical...mentioning

confidence: 99%

Recent Progress in Functional-Nucleic-Acid-Based Fluorescent Fiber-Optic Evanescent Wave Biosensors

Wang

Lou

2023

Biosensors

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Biosensors capable of onsite and continuous detection of environmental and food pollutants and biomarkers are highly desired, but only a few sensing platforms meet the “2-SAR” requirements (sensitivity, specificity, affordability, automation, rapidity, and reusability). A fiber optic evanescent wave (FOEW) sensor is an attractive type of portable device that has the advantages of high sensitivity, low cost, good reusability, and long-term stability. By utilizing functional nucleic acids (FNAs) such as aptamers, DNAzymes, and rational designed nucleic acid probes as specific recognition ligands, the FOEW sensor has been demonstrated to be a general sensing platform for the onsite and continuous detection of various targets ranging from small molecules and heavy metal ions to proteins, nucleic acids, and pathogens. In this review, we cover the progress of the fluorescent FNA-based FOEW biosensor since its first report in 1995. We focus on the chemical modification of the optical fiber and the sensing mechanisms for the five above-mentioned types of targets. The challenges and prospects on the isolation of high-quality aptamers, reagent-free detection, long-term stability under application conditions, and high throughput are also included in this review to highlight the future trends for the development of FOEW biosensors capable of onsite and continuous detection.

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Section: The Major Components Of Fluorescent Foew Sensors and Optical...mentioning

confidence: 99%

Recent Progress in Functional-Nucleic-Acid-Based Fluorescent Fiber-Optic Evanescent Wave Biosensors

Wang

Lou

2023

Biosensors

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“…A neoteric approach for sensitivity enhancement employed by Akhtar's group includes growing ZnO nanoflowers on a glass slide coated with silver nanofilm for ultrasensitive detection of insulin amyloid [178]. Sophisticated assemblies such as dual-colour TIR fluorescence detecting platform [179] as well as optical fibre-polymer waveguide-optical fibre sandwich [180] set up have facilitated small-molecule detection down to picomolar levels.…”

Section: Evanescent Wave-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

“…Micromachines 2020, 11, x FOR PEER REVIEW 13 of 31 fluorescence detecting platform [179] as well as optical fibre-polymer waveguide-optical fibre sandwich [180] set up have facilitated small-molecule detection down to picomolar levels.…”

Section: Bioluminescence-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

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“…Our group recently developed a portable platform (i.e., the evanescent wave optofluidic immunosensing platform, OIP) that combines the advantages of evanescent wave fluorescence, microfluidics, and immunoassay [ 17 ]. Compared with other evanescent wave fluorescence biosensors (e.g., RAPTOR [ 18 ] and Analyte 2000 [ 19 ]), the OIP had a simpler and more compact optical structure, in which no rigorous optical alignment was required. The OIP possesses great potential for detecting various pollutants because of its several unique characteristics including low reagent consumption, rapidity, reproducibility, and portability, which are also the desired characteristics for on-site detection of food pollutants.…”

Section: Introductionmentioning

confidence: 99%

“…The hapten-carrier protein conjugates were covalently immobilized onto the bio-probe surface as biorecognition molecules using N,N′-disuccinimidyl carbonate (DSC) as the bifunctional reagent instead of other bifunctional reagents (e.g., glutaraldehyde). Second, a low-cost, Si-based photodiode detector (SOP-1000) with small volume and high sensitivity was used as an alternative to a photomultiplier tube (PMT) or lock-in amplifier for the detection of a weak fluorescence signal [ 18 ]. This not only reduced the cost instrument, but it also allowed for improved portability due to its small size.…”

Section: Introductionmentioning

confidence: 99%

Rapid, Sensitive On-Site Detection of Deoxynivalenol in Cereals Using Portable and Reusable Evanescent Wave Optofluidic Immunosensor

Liu

Chen

et al. 2022

IJERPH

Self Cite

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This paper develops an improved portable and reusable evanescent wave optofluidic immunosensor (OIP-v2) for rapid and sensitive on-site determination of deoxynivalenol (DON), one of the most frequently detected mycotoxins mainly produced by Fusarium species. Using the bifunctional reagent N,N′-Disuccinimidyl carbonate, deoxynivalenol-bovine-serum-albumin (DON-BSA) were covalently modified onto a bio-probe surface as biorecognition elements, whose robustness allowed it to perform multiple detections without significant activity loss. An indirect competitive immunoassay strategy was applied for DON detection. Under optimal conditions, the limit of detection of 0.11 μg/L and the linear dynamic detection range of 0.43 to 36.61 μg/L was obtained when the concentration of the Cy5.5-anti-DON antibody was 0.25 μg/mL. The OIP-v2 was also applied to detect DON in various cereals, and the recoveries ranged from 81% to 127%. The correlation between OIP-v2 and enzyme-linked immunosorbent assay (ELISA) through the simultaneous detection of maize-positive samples was in good agreement (R2 = 0.9891).

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A novel dual-color total internal reflection fluorescence detecting platform using compact optical structure and silicon-based photodetector

Cited by 9 publications

References 25 publications

Recent Progress in Functional-Nucleic-Acid-Based Fluorescent Fiber-Optic Evanescent Wave Biosensors

Recent Progress in Functional-Nucleic-Acid-Based Fluorescent Fiber-Optic Evanescent Wave Biosensors

Recent Progress in Optical Sensors for Biomedical Diagnostics

Rapid, Sensitive On-Site Detection of Deoxynivalenol in Cereals Using Portable and Reusable Evanescent Wave Optofluidic Immunosensor

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