A novel dodecadepsipeptide, cereulide, is an emetic toxin of<i>Bacillus cereus</i>

Agata, Norio; Ohta, Michio; Mori, Masashi; Isobe, Mitsuaki

doi:10.1111/j.1574-6968.1995.tb07550.x

Cited by 145 publications

(172 citation statements)

References 1 publication

Supporting

Mentioning

167

Contrasting

Unclassified

Order By: Relevance

“…Indeed, 99n0 % of the cells were dead after incubation with supernatant from the parental strain, 407 Cry − , whereas only 5n3 % of cells were dead after treatment with a supernatant from the 407 Cry − ∆plcR mutant. The vacuole formation observed with the supernatant of the 407 Cry − ∆plcR mutant was not dependent on PlcR-regulated functions and may have been due to other compounds, such as the B. cereus emetic toxins, which are known to cause vacuole formation in eukaryotic cells (Agata et al, 1995). In contrast, sterile LB and the supernatant of a B. subtilis strain, used as controls, had no cytolytic effect on G. mellonella haemocytes (Fig.…”

Section: Cytotoxic and Haemolytic Activities Are Controlled By Plcrmentioning

confidence: 81%

The plcR regulon is involved in the opportunistic properties of Bacillus thuringiensis and Bacillus cereus in mice and insects

Ramisse²,

et al. 2000

View full text Add to dashboard Cite

Bacillus thuringiensis has been widely used for 40 years as a safe biopesticide for controlling agricultural pests and mosquitoes because it produces insecticidal crystal proteins. However, spores have also been shown to contribute to overall entomopathogenicity. Here, the opportunistic properties of acrystalliferous B. thuringiensis Cry N and Bacillus cereus strains were investigated in an insect species, Galleria mellonella, and in a mammal, BALB/c mice. In both animal models, the pathogenicity of the two bacterial species was similar. Mutant strains were constructed in which the plcR gene, encoding a pleiotropic regulator of extracellular factors, was disrupted. In larvae, coingestion of 10 6 spores of the parental strain with a sublethal concentration of Cry1C toxin caused 70 % mortality whereas only 7 % mortality was recorded if spores of the ∆plcR mutant strain were used. In mice, nasal instillation of 10 8 spores of the parental strain caused 100 % mortality whereas instillation with the same number of ∆plcR strain spores caused much lower or no mortality. Similar effects were obtained if vegetative cells were used instead of spores.The cause of death is unknown and is unlikely to be due to actual growth of the bacteria in mice. The lesions caused by B. thuringiensis supernatant in infected mice suggested that haemolytic toxins were involved. The cytolytic properties of strains of B. thuringiensis and B. cereus, using sheep, horse and human erythrocytes and G. mellonella haemocytes, were therefore investigated. The level of cytolytic activity is highly reduced in ∆plcR strains.Together, the results indicate that the pathogenicity of B. thuringiensis strain 407 and B. cereus strain ATCC 14579 is controlled by PlcR.

show abstract

Section: Cytotoxic and Haemolytic Activities Are Controlled By Plcrmentioning

confidence: 81%

The plcR regulon is involved in the opportunistic properties of Bacillus thuringiensis and Bacillus cereus in mice and insects

Ramisse²,

et al. 2000

View full text Add to dashboard Cite

show abstract

“…This strain however, did not harbour cesA gene as revealed by cesA-specific PCR using primers CER1 and EMT1. Cereulide is a small heat stable cyclic dodecadepsipeptide produced by some strains of B. cereus which has high toxicity to humans (1,36). Cereulide is synthesized by a non-ribosomal peptide synthetase, encoded by the ces genes located on a 270-kb pXOI-like virulence plasmid named pCER270 (10,26), more often found associated with clinical isolates of B. cereus.…”

Section: Discussionmentioning

confidence: 99%

“…were isolated and identified by biochemical tests and fatty acid methyl ester analysis (FAME). These included B. pumilus (16) (1) and Bacillus GC group 22 (1). The Bacillus GC group 22 corresponds to the gas chromatographic profile of a Bacillus species in the Sherlock TSBA Library version 3.9 (Microbial ID, MIDI Inc.), the 16S rDNA sequence of which does not match any known species of the genus Bacillus.…”

Section: Taxonomic Identification Of Bacillus Spp Isolated From Coasmentioning

confidence: 99%

Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India

Parvathi¹,

Krishna²,

Jose³

et al. 2009

Braz. J. Microbiol.

View full text Add to dashboard Cite

Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical methods, fatty acid methyl ester (FAME) analysis and partial 16S rDNA sequencing. Analysis of the data revealed that Bacillus pumilus was the most predominant species in the region under study followed by B. cereus and B. sphaericus. The B. pumilus isolates were further characterized by arbitrarily primed PCR (AP-PCR), antibiotic sensitivity profiling and PCR screening for known toxin genes associated with Bacillus spp. All B. pumilus isolates were biochemically identical, exhibited high protease and lipase activity and uniformly sensitive to antibiotics tested in this study. One strain of B. pumilus harboured cereulide synthetase gene cesB of B. cereus which was indistinguishable from rest of the isolates biochemically and by AP-PCR. This study reports, for the first time, the presence of the emetic toxin gene cesB in B. pumilus.

show abstract

“…To detect the virulence genes of BSI isolates, polymerase chain reaction (PCR) assays were performed with the enterotoxin FM (entFM) gene [16], the enterotoxin T (bceT) gene [17], the haemolytic enterotoxin complex (hblACD) genes [18], the non-haemolytic enterotoxin (NHE) complex (nheABC) genes, the cytK gene [11] and the piplc gene [19].…”

Section: Virulence Factorsmentioning

confidence: 99%

Erratum to: Seasonal trend and clinical presentation of Bacillus cereus bloodstream infection: association with summer and indwelling catheter

Kato

Matsumura

Yamamoto

et al. 2016

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

Bacillus cereus, an opportunistic pathogen, can cause fatal infection. However, B. cereus bloodstream infections (BSIs) have not been well characterised. From 2008 to 2013, B. cereus isolates from all of the specimens and patients with B. cereus BSIs were identified. Environmental samples were collected to detect B. cereus contamination. We also characterised the clinical presentation of B. cereus BSI through analyses of risk factors for BSI and mortality. A total of 143 clinical B. cereus isolates was detected. Fifty-one patients with nosocomial infections were diagnosed as B. cereus BSI, and 37 had contaminated blood cultures. The number of B. cereus isolates and BSI patients was significantly greater from June to September than from January to April (3.4 vs. 1.0 per month and 1.4 vs. 0.2, respectively). All BSIs were nosocomial and related to central or peripheral vascular catheter. Urinary catheter [odds ratio (OR) 6.93, 95 % confidence interval (CI) 2.40-20.0] was the independent risk factor associated with BSI patients when compared to patients regarded as contaminated. In-hospital mortality among BSI patients was 20 % and was associated with urinary catheter (OR 12.3, p = 0.045) and higher Charlson index (OR 1.99,). The number of B. cereus isolates and BSI increased during summer. Inpatients with indwelling vascular or urinary catheters should be carefully monitored for potential B. cereus BSIs.

show abstract

A novel dodecadepsipeptide, cereulide, is an emetic toxin ofBacillus cereus

Cited by 145 publications

References 1 publication

The plcR regulon is involved in the opportunistic properties of Bacillus thuringiensis and Bacillus cereus in mice and insects

The plcR regulon is involved in the opportunistic properties of Bacillus thuringiensis and Bacillus cereus in mice and insects

Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India

Erratum to: Seasonal trend and clinical presentation of Bacillus cereus bloodstream infection: association with summer and indwelling catheter

Contact Info

Product

Resources

About