A novel DNA biosensor based on ssDNA/Cyt c/l-Cys/GNPs/Chits/GCE

Li, Mengyao; Huang, Shasheng; Zhu, Peisi; Kong, Lingmi; Peng, Bin; Gao, Huan

doi:10.1016/j.electacta.2008.10.040

Cited by 30 publications

(16 citation statements)

References 40 publications

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“…For comparison purpose, SERS spectrum of R6G on the solid gold nanoparticles (size distribution from 30 nm to 70 nm, average size of 43 nm) prepared according to the procedure described by Turkevich 22 was also recorded. According to the procedure reported elsewhere, 23 a DNA biosensor was fabricated by using sample A HGNs and denoted as ssDNA/Cyt c/L-Cys/HGNs/Chits/GCE. This DNA biosensor was hybridized in 2.1×10 -8 mol•L -1 complementary and non-complementary DNA solution for 3 h, respectively, and then incubated in 1.0×10…”

Section: Samples For Sers and Biosensor Experimentsmentioning

confidence: 99%

Synthesis of Hollow Gold Nanospheres and Their Applications in Surface‐enhanced Raman Scattering and DNA Biosensor

Meng

Yang

et al. 2010

Chin. J. Chem.

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Hollow gold nanospheres (HGNs) with nanopinholes were prepared by chemical reduction of HAuCl 4 with aqueous KBH 4 in the presence of the Bu 4 PBr template. The HGNs could be served as substrates for surface-enhanced Raman scattering (SERS), which exhibited much higher surface enhancement than the solid gold nanoparticles in case of Rhodamine 6G as a molecule probe. Moreover, the HGNs could also be used in the modified electrode to detect DNA and superior to the solid counterpart of the similar size. Based on the detailed characterizations by transmission electron micrograph (TEM), field emission scanning electron micrograph (FESEM) and UV-visible absorption spectroscopy, a plausible mechanism for forming HGNs was proposed. Meanwhile, the promoting effect of the hollow gold nanospheres on the SERS was discussed briefly.

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Section: Samples For Sers and Biosensor Experimentsmentioning

confidence: 99%

Synthesis of Hollow Gold Nanospheres and Their Applications in Surface‐enhanced Raman Scattering and DNA Biosensor

Meng

Yang

et al. 2010

Chin. J. Chem.

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“…20-mer thiolated probe DNA immobilized on electrodeposited poly(propyleneimine) doped with gold nanoparticles on glassy carbon electrode was prepared Arotiba et al The nanosensor responded to target DNA in a dynamic linear range of 0.01-5 nM using [Fe(CN) 6 ] 3-/4-redox probe [55]. A DNA hybridization biosensor based on ssDNA/cytochrome c/L-cysteine/gold nanoparticles/chitosan monolayer modified on glassy carbon electrode by Li et al The amount of adsorbed DNA was found as 1.672 x 10 -10 mol/cm 2 based on DPV response of daunomycin indicator [8].…”

Section: Studies Based On Both Nanoparticles and Polymersmentioning

confidence: 98%

“…Electrochemical *Address correspondence to this author at the Analytical Chemistry Department, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey; Tel: +90-232-311 10 10 Ext. 5131; Fax: +90-232-388 5258; E-mail: arzum.erdem@ege.edu.tr methods including cyclic voltammetry, differential pulse voltammetry, square wave voltammetry, amperometry, impedance spectroscopy and chrono-potentiometry are commonly used for monitoring DNA hybridization [8].…”

Section: Introductionmentioning

confidence: 99%

The Recent Electrochemical Biosensor Technologies for Monitoring of Nucleic Acid Hybridization

Karadeniz¹,

Kuralay²,

Abacı³

et al. 2011

CAC

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Electrochemical biosensors have a key importance in many areas including clinical, biological, pharmaceutical, forensic, environmental and agricultural applications. They have the advantages of rapid, easy and low cost preparation allowing the localization of biocompound on electrodes of any size with independence of sample turbidity, high sensitivity and high selectivity. Electrochemical devices have attracted considerable interest in the development of DNA hybridization biosensors. They rely on the conversion of hybridization event into a direct electrochemical signal. Many efforts have been carried out in the detection of DNA hybridization for improving the stability, reproducibility and sensitivity.This review summarizes the advanced electrochemical methodologies involved in nucleic acid hybridization performed by using polymer technology, nanoparticles (NPs), magnetic particles, and carbon nanotubes (CNTs). The preparation of polymer modified electrodes especially including conducting polymers and their applications for DNA hybridization are given with the advantages of electrocatalysis, good electroactivity, high sensitivity and providing suitable matrix for DNA immobilization. The role of various nanomaterials in biosensors with the advantages of high surface area, fast heterogeneous electron transfer, excellent biocompatibility and enhanced signal with a good selectivity are presented. The combination of these techniques is also discussed in details.

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“…27 The position of the Soret absorption band of Cyt c can provide information about possible denaturation of Cyt c, especially conformational change in the heme region. 31 According to literature 32 these bands are p-p* essentially band associated with the porphyrin ring which is present in Cyt c. Both dry Cyt c and the porous Cyt c-CA lms on quartz slides showed the absorption bands at 409 nm, 530 nm and 560 nm with a similar peak shape ( Fig. The UV-vis spectra of Cyt c exhibits three major bands, the Soret band at 409 nm, a band at 530 nm, and b band located at 560 nm.…”

Section: Conformational Studiesmentioning

confidence: 99%

The electrochemical studies of cytochrome c incorporated in 3D porous calcium alginate films on glassy carbon electrodes

Jian¹,

Liu²,

Sun³

et al. 2014

RSC Adv.

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In this work, the cytochrome c (Cyt c) was incorporated in three-dimension (3D) porous calcium alginate (CA) films prepared by using sodium alginate, calcium chloride and polyethylene glycol, and formed the electroactive porous Cyt c-CA composite films. The stable composite films were characterized by means of scanning electron microscopy (SEM), UV-vis absorption, cyclic voltammetry (CV) and squarewave voltammetry (SWV) techniques. UV-vis experiments demonstrated that Cyt c assembled in the 3D porous CA films retains its near native conformation at medium pH. The Cyt c-CA films modified on glassy carbon electrodes showed a pair of well-defined and nearly reversible cyclic voltammetry peaks at about À0.352 V vs. SCE in protein-free buffer, which originated from heme Fe III/II redox couples within Cyt c. The electrochemical parameters such as apparent heterogeneous electron transfer rate constant (k s ) and formal potential (E o 0 ) were estimated by fitting the data of SWV with nonlinear regression analysis. The Cyt c-CA films showed the electrocatalytic activity toward dioxygen, hydrogen peroxide, nitrite and trichloroacetic acid with significant decreases in the electrode potential required. Experimental data demonstrated that the porous film can provide a favorable microenvironment for the protein to directly transfer electrons with the underlying electrode and possess good stability and reproducibility, showing the possible future application of the films for biosensors and biocatalysis.

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A novel DNA biosensor based on ssDNA/Cyt c/l-Cys/GNPs/Chits/GCE

Cited by 30 publications

References 40 publications

Synthesis of Hollow Gold Nanospheres and Their Applications in Surface‐enhanced Raman Scattering and DNA Biosensor

Synthesis of Hollow Gold Nanospheres and Their Applications in Surface‐enhanced Raman Scattering and DNA Biosensor

The Recent Electrochemical Biosensor Technologies for Monitoring of Nucleic Acid Hybridization

The electrochemical studies of cytochrome c incorporated in 3D porous calcium alginate films on glassy carbon electrodes

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