A novel DNA binding protein-based platform for electrochemical detection of miRNA

Umer, Muhammad; Aziz, Nahian Binte; Mahmudunnabi, Rabbee G.; Shim, Yoon‐Bo; Salomón, Carlos; Shiddiky, Muhammad J. A.

doi:10.1039/d1an00935d

Cited by 8 publications

(3 citation statements)

References 27 publications

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“…Electrochemical detection, which is characterized by high sensitivity, rapid response, good selectivity [ 13 ], and simplicity of operation, has been employed by researchers for microRNA detection [ 14 – 20 ]. However, conventional heterogeneous electrochemical biosensors typically involve the immobilization of a probe on a working electrode, which requires laborious and time-consuming electrode pretreatment and probe immobilization steps [ 21 – 25 ].…”

Section: Introductionmentioning

confidence: 99%

Label-Free Ratiometric Homogeneous Electrochemical Strategy Based on Exonuclease III-Aided Signal Amplification for Facile and Rapid Detection of miR-378

Fan,

Wang,

Wang

et al. 2024

International Journal of Analytical Chemistry

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MiR-378 is abnormally expressed in various cancers, such as hepatocellular carcinoma, renal cell carcinoma, and nonsmall cell lung cancer. Here, we developed a label- and immobilization-free ratiometric homogeneous electrochemical strategy based on exonuclease III (Exo III) for the facile and rapid determination of miR-378. Two 3′-protruding hairpin DNA probes (HPs) are designed in this strategy. Doxorubicin (DOX) and potassium ferrocyanide (Fe2+) were used as label-free probes to produce a response signal (IDOX) and a reference signal (IFe2+) in the solution phase. When no target was present in the solution, the HP was stable, most of the DOX was intercalated in the stem of the HP, and the diffusion rate of DOX was significantly reduced, resulting in reduced electrochemical signal response. When miR-378 was present, double-cycle signal amplification triggered by Exo III cleavage was initiated, ultimately disrupting the hairpin structures of HP1 and HP2 and releasing a large amount of DOX into the solution, yielding a stronger electrochemical signal, which was low to 50 pM. This detection possesses excellent selectivity, demonstrating high application potential in biological systems, and offers simple and low-cost electrochemical detection for miR-378.

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Section: Introductionmentioning

confidence: 99%

Label-Free Ratiometric Homogeneous Electrochemical Strategy Based on Exonuclease III-Aided Signal Amplification for Facile and Rapid Detection of miR-378

Fan,

Wang,

Wang

et al. 2024

International Journal of Analytical Chemistry

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“…The amperometric signal was monitored in presence of hydroquinone and H 2 O 2 , obtaining a limit of detection equal to 22 fM. [25] Shiddiky's research group developed different amplificationfree electrochemical approaches for the detection of miRNA using screen-printed gold electrodes. The first device relies on gold-loaded nanoporous superparamagnetic iron oxide nanocubes for the detection of miRNA-107 (Au-NPFe 2 O 3 NC).…”

Section: Introductionmentioning

confidence: 99%

“…The amperometric signal was monitored in presence of hydroquinone and H 2 O 2 , obtaining a limit of detection equal to 22 f m . [ 25 ]…”

Section: Introductionmentioning

confidence: 99%

Liquid Biopsy beyond Cancer: A miRNA Detection in Serum with Electrochemical Chip for Non‐Invasive Coeliac Disease Diagnosis

Caratelli

Moccia

Paggioro

et al. 2022

Advanced NanoBiomed Research

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Coeliac disease is a very common autoimmune disease estimated to affect 1 in 100 people worldwide. It occurs in genetically predisposed people where the ingestion of gluten leads to damage in the small intestine, and it is accurately diagnosticated through duodenal biopsy, an invasive diagnostic method. The liquid biopsy, generally used for monitoring cancer, is an appealing alternative even for autoimmune pathology such as coeliac disease, allowing for detecting disease progression or resistance to treatment. For this reason, an electrochemical peptide nucleic acid (PNA) device combined with a smartphone‐assisted potentiostat for non‐invasive coeliac disease diagnosis is proposed, by measuring the selected overexpressed miRNA‐486‐5p in serum, enlarging the application of liquid biopsy in nontumor pathologies. For highly sensitive detection, the polyester‐based printed sensor is nanomodified with gold nanoparticles and a synthetic customized PNA probe. The designed sensor can detect the target analyte in the range of 10–100 nm with a limit of detection of 0.7 nm by measuring the variation of the response of the electrochemical mediator hexaammineruthenium in the presence of PNA–miRNA duplex on the nanostructured working electrode surface. The analyses testing serum samples are found in agreement with ones obtained by real‐time quantitative polymerase chain reaction (RT‐qPCR), demonstrating the reliability of this innovative electrochemical chip developed.

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Electrochemical Biosensor for Ultrasensitive Detection of Microrna‐21 through In Situ Synthesis of Palladium Nanoparticles

Huang,

Ma,

Kong

et al. 2023

ChemistrySelect

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The detection of trace microRNAs (miRNA) is crucial for early cancer diagnosis. Here, a sensitive biosensor for miRNA directive detection was put forward, which was based on RNA‐templated palladium nanoparticles (PdNPs) signal amplification strategy. Uncharged peptide nucleic acids (PNA) probe was used to capture the target miRNA(T‐RNA) specifically. Subsequently, a large amount of palladium ions absorbed on T‐RNA strands via electrostatic forces, and the palladium nanocluster is rapidly generated based on chemical reduction. Obviously, RNA metallization method enables biosensor to be carried out efficiently at room temperature also simplifies the preparation and detection of the sensor. Under optimal conditions, this method can analyze the concentration of miRNA in the range of 10 fM to 1 nM, and the detection limit is 3.33 fM. At the same time, this method has strong anti‐interference ability in serum samples, which provides a broad prospect for the early screening and diagnosis of cancer.

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A novel DNA binding protein-based platform for electrochemical detection of miRNA

Abstract: We present a novel amplification-free sandwich type platform assay for electrochemical detection of miRNA. The assay is based on T4 DNA polymerase mediated synthesis of p53 binding DNA sequence at...

Cited by 8 publications

References 27 publications

Label-Free Ratiometric Homogeneous Electrochemical Strategy Based on Exonuclease III-Aided Signal Amplification for Facile and Rapid Detection of miR-378

Label-Free Ratiometric Homogeneous Electrochemical Strategy Based on Exonuclease III-Aided Signal Amplification for Facile and Rapid Detection of miR-378

Liquid Biopsy beyond Cancer: A miRNA Detection in Serum with Electrochemical Chip for Non‐Invasive Coeliac Disease Diagnosis

Electrochemical Biosensor for Ultrasensitive Detection of Microrna‐21 through In Situ Synthesis of Palladium Nanoparticles

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