A novel diazoresin/polyethylene glycol covalent capillary coating for analysis of proteins by capillary electrophoresis

Abstract

A novel method for the preparation of covalently linked capillary coatings of polyethylene glycol (PEG) was demonstrated using photosensitive diazoresin (DR) as the coupling agent. Layer by layer (LBL) selfassembly film of DR and PEG based on hydrogen bonding was first fabricated on the inner wall of capillary, then the hydrogen bonding was converted into covalent bonding after treatment with UV light through a unique photochemistry reaction of DR. The covalently bonded coatings suppressed protein adsorption on the inner surface of the capillary, and thus a baseline separation of lysozyme (Lys), cytochrome c (Cyt-c), bovine serum albumin (BSA) and ribonuclease A (RNase A) was achieved using capillary electrophoresis (CE). Compared with the bare capillary or non-covalently bonded DR/PEG coatings, the covalently linked DR/PEG capillary coatings not only improved the CE separation performance for proteins, but also exhibited good stability and repeatability. Due to the replacement of highly toxic and moisture sensitive silane coupling agents by DR in the covalent coating preparation, this method may provide a green and easy way to make the covalently coated capillaries for CE.

Sugar fractionation using surface-modified nanofiltration membranes

“…6, covalently linked capillary coating of PSS is prepared using the layer by layer self-assembly technique combined with photochemistry reactions. PSS coating based on ionic bonding was first assembled on the membrane surface by using photosensitive diazoresin (DR) as the coupling agent, and then the ionic bonding was converted into covalent bonding after treatment with UV light through a unique photochemistry reaction of DR [43][44][45]. Compared with uncrosslinked [PSS/PDADMAC] n PSS coating, the covalent PSS/DR coating has good stability and reproducibility.…”

Recent advances in protein analysis by capillary and microchip electrophoresis

“…The Diazo-PEG provided higher and more stable EOF suppression than DR/PEG previously reported by the same group. 48 …”

Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis

“…An example of such a process was described by Yu et al using a photosensitive diazoresin (DR) in combination with either PVA [29] or polyethylene glycol (PEG) [30]. After exposure to 365 nm light, both the DR/PVA and DR/PEG coatings were able to prevent protein adsorption and achieve an efficient separation of several model basic proteins (Table 1) with a migration time precision less than 4% RSD.…”