A novel detection method of infection in cattle based on 1

Tai, Lixin; Li, Jianhua; Yin, Jun; Zhang, Nan; Ju, Yang; He, Lin; Yang, Zhengtao; Gong, Pengtao; Zhang, Xichen

doi:10.1093/abbs/gmy143

Cited by 5 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, virus RdRp has more diversity than the capsid gene of CSpV1. Since there is no efficient and reliable differential diagnosis to determine Cryptosporidium by sub‐species level, we and others suggest that virus RdRp‐directed molecular or immune assay methods could be useful to achieve this goal (Tai et al., 2019). Targeting the symbiont viruses to produce efficient vaccine against cryptosporidiosis is a novel strategy that may produce successful vaccines.…”

Section: Discussionmentioning

confidence: 99%

Newly identified Cryptosporidium parvum virus‐1 from newborn calf diarrhoea in Turkey

Berber

Şimşek

Çanakoğlu

et al. 2020

Transbound Emerg Dis

View full text Add to dashboard Cite

Cryptosporidium is a common enteric parasite that primarily affects those immunocompromised susceptible individuals and newborns. Detailed investigations have revealed that Cryptosporidium (C.) oocysts contain dsRNA segments which are recently classified under the Partitiviridae family. The relationship between parasite and virus whether or not affect the clinical outcomes of newborn calf diarrhoea is not apparent. The aim of this study was the identification and characterization of Cryptosporidium parvum virus-1 (CSpV1) from newborn calves. We also aimed to understand that parasite-virus symbiont relationship role in the severity of disease cases. Parasitic screening was performed with the help of morphological examinations, immunoassay and molecular polymerase chain reaction (PCR) methods. To further identification of C. parvum oocysts, confocal laser, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) image analysis were used for the morphological investigations. Software-based in silico comparison and identity analyses were conducted from the CSpV1 genome for the genomic sequence characterizations. Cryptosporidium prevalence was 56.2% in newborn calf diarrhoeal cases.Virus dsRNA segments isolated from purified and clarified oocysts. Sequence results showed that we have successfully isolated CSpV1 from C. parvum oocysts. Virus RNA-dependent RNA polymerase (RdRp) was found to be highly variable and showed a species-specific relationship with their carriers. We also identified that CSpV1 frequency was around 8.8% from diarrhoea-showing newborn calves. Cryptosporidium was strongly associated with diarrhoea at early ages of newborns, but the parasite and CSpV1 relationship is not associated with the severity of newborn calf diarrhoea.The current study provides the first report and molecular characterization of CSpV1 in Turkey.

show abstract

Section: Discussionmentioning

confidence: 99%

Newly identified Cryptosporidium parvum virus‐1 from newborn calf diarrhoea in Turkey

Berber

Şimşek

Çanakoğlu

et al. 2020

Transbound Emerg Dis

View full text Add to dashboard Cite

show abstract

“…Based on dsRNA2 sequence comparison, a greater divergence exists between CSpV1 and viruses from C. hominis, C. felis and C. meleagridis, suggesting that the virus might have a certain degree of host-specificity and, therefore, the existence of more than one species in the genus Cryspovirus [104]. Infection by CSpV1 is commonly detected in field and clinical isolates of C. parvum [105]. A correlation between CSpV1 dsRNA2 levels and C. parvum fecundity has been reported in culture models, where higher level of the viral symbiont are associated with greater parasite multiplication [106].…”

Section: Other Viruses Of Protozoan Parasites Of Medical Relevancementioning

confidence: 99%

Viruses of protozoan parasites and viral therapy: Is the time now right?

Barrow

Dujardin

Fasel

et al. 2020

Virol J

View full text Add to dashboard Cite

Infections caused by protozoan parasites burden the world with huge costs in terms of human and animal health. Most parasitic diseases caused by protozoans are neglected, particularly those associated with poverty and tropical countries, but the paucity of drug treatments and vaccines combined with increasing problems of drug resistance are becoming major concerns for their control and eradication. In this climate, the discovery/repurposing of new drugs and increasing effort in vaccine development should be supplemented with an exploration of new alternative/synergic treatment strategies. Viruses, either native or engineered, have been employed successfully as highly effective and selective therapeutic approaches to treat cancer (oncolytic viruses) and antibiotic-resistant bacterial diseases (phage therapy). Increasing evidence is accumulating that many protozoan, but also helminth, parasites harbour a range of different classes of viruses that are mostly absent from humans. Although some of these viruses appear to have no effect on their parasite hosts, others either have a clear direct negative impact on the parasite or may, in fact, contribute to the virulence of parasites for humans. This review will focus mainly on the viruses identified in protozoan parasites that are of medical importance. Inspired and informed by the experience gained from the application of oncolytic virus- and phage-therapy, rationally-driven strategies to employ these viruses successfully against parasitic diseases will be presented and discussed in the light of the current knowledge of the virus biology and the complex interplay between the viruses, the parasite hosts and the human host. We also highlight knowledge gaps that should be addressed to advance the potential of virotherapy against parasitic diseases.

show abstract

“…A single study has assessed the cryspovirus prevalence in parasite populations, in Japan [ 25 ]. It showed that CSpV1 has 100% apparent prevalence and appears to co-diversify with C. parvum so that viral sequences might be used for sensitive detection of C. parvum subtype IIaA15G2R1 and to determine the regional origin of the parasite [ 25 – 27 ].…”

Section: Introductionmentioning

confidence: 99%

First identification of Cryptosporidium parvum virus 1 (CSpV1) in various subtypes of Cryptosporidium parvum from diarrheic calves, lambs and goat kids from France

Adjou,

Chevillot,

Lucas

et al. 2023

Vet Res

View full text Add to dashboard Cite

Cryptosporidium spp. remain a major cause of waterborne diarrhea and illness in developing countries and represent a significant burden to farmers worldwide. Cryptosporidium parvum virus 1 (CSpV1), of the genus Cryspovirus, was first reported to be present in the cytoplasm of C. parvum in 1997. Full-length genome sequences have been obtained from C. parvum from Iowa (Iowa), Kansas (KSU) and China. We aimed at characterizing the genome of CSpV1 from France and used sequence analysis from Cryptosporidium isolates to explore whether CSpV1 genome diversity varies over time, with geographical sampling location, C. parvum genetic diversity, or ruminant host species. A total of 123 fecal samples of cattle, sheep and goats were collected from 17 different French departments (57 diseased animal fecal samples and 66 healthy animal fecal samples). Subtyping analysis of the C. parvum isolates revealed the presence of two zoonotic subtype families IIa and IId. Sequence analysis of CSpV1 revealed that all CSpV1 from France, regardless of the subtype of C. parvum (IIaA15G2R1, IIaA17G2R1 and IIdA18G1R1) are more closely related to CSpV1 from Turkey, and cluster on a distinct branch from CSpV1 collected from C. parvum subtype IIaA15G2R1 from Asia and North America. We also found that samples collected on a given year or successive years in a given location are more likely to host the same subtype of C. parvum and the same CSpV1 strain. Yet, there is no distinct clustering of CSpV1 per French department or ruminants, probably due to trade, and transmission of C. parvum among host species. Our results point towards (i) a close association between CSpV1 movement and C. parvum movement, (ii) recent migrations of C. parvum among distantly located departments and (iii) incidental transmission of C. parvum between ruminants. All together, these results provide insightful information regarding CSpV1 evolution and suggest the virus might be used as an epidemiological tracer for C. parvum. Future studies need to investigate CSpV1’s role in C. parvum virulence and on subtype ability to infect different species.

show abstract

A novel detection method of infection in cattle based on 1

Cited by 5 publications

References 20 publications

Newly identified Cryptosporidium parvum virus‐1 from newborn calf diarrhoea in Turkey

Newly identified Cryptosporidium parvum virus‐1 from newborn calf diarrhoea in Turkey

Viruses of protozoan parasites and viral therapy: Is the time now right?

First identification of Cryptosporidium parvum virus 1 (CSpV1) in various subtypes of Cryptosporidium parvum from diarrheic calves, lambs and goat kids from France

Contact Info

Product

Resources

About