A novel design of microfluidic platform for metronomic combinatorial chemotherapy drug screening based on 3D tumor spheroid model

Sankar, Sharanya; Mehta, Viraj; Ravi, Subhashini; Sharma, Chandra Shekhar; Rath, Subha Narayan

doi:10.1007/s10544-021-00593-w

Cited by 9 publications

(5 citation statements)

References 25 publications

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“…The 3D spheroid system has gained popularity due to the representation of physicochemical gradients as seen in real tumors which proved to be helpful in deciphering the effect of anticancer agents. 59 However, many studies show the reciprocal interaction of spheroids with the ECM influencing the drug sensitivity. 60,61 However, recent evidence highlights the utilization of tissue-specific decellularized scaffolds in tumor engineering of organotypic models and organ-specific metastases to facilitate drug discovery and screening.…”

Section: Generation Of Patient-derived 3d Oral Spheroids From Cancer ...mentioning

confidence: 99%

Osteomatrix as a personalized 3D tissue-specific invasion test-bed for oral carcinoma

Sukanya

Giri

Nellore³

et al. 2023

Biomater. Sci.

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Section: Generation Of Patient-derived 3d Oral Spheroids From Cancer ...mentioning

confidence: 99%

Osteomatrix as a personalized 3D tissue-specific invasion test-bed for oral carcinoma

Sukanya

Giri

Nellore³

et al. 2023

Biomater. Sci.

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“…Thus, MBs close to and away from the chamber are removed (Figure 1a(ii)). The removal rate dV/dt through a thin PDMS wall can be theoretically expressed as follows [15]:…”

Section: Working Principle Of the Removal Of Mbsmentioning

confidence: 99%

“…However, the long-term culture of organoids and tissues is challenging because of culture conditions and waste accumulation. Therefore, perfusion cultures of the organoids and tissues were developed using microfluidic devices [14][15][16][17][18], which enable the expression of actual behaviors and long-term cell culture. However, microbubbles (MBs, optically visible at the micro scale) often form in microfluidic devices, change/stop the flow of culture medium in a microchannel, and apply surface tension to cells when MBs and cells are brought into contact.…”

Section: Introductionmentioning

confidence: 99%

Local Microbubble Removal in Polydimethylsiloxane Microchannel by Balancing Negative and Atmospheric Pressures

Tokuoka,

Ishida

2023

Micromachines

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Long-term experiments using organoids and tissues are crucial for drug development. Microfluidic devices have been regularly used in long-term experiments. However, microbubbles often form in these devices, and they may damage and starve cells. A method involving the application of negative pressure has been reported to remove microbubbles from microfluidic devices composed of polydimethylsiloxane; however, negative pressure affects the cells and tissues in microfluidic devices. In this study, a local microbubble removal method was developed using a microfluidic device with 0.5 mm thin polydimethylsiloxane sidewalls. The thin sidewalls counterbalanced the negative and atmospheric pressures, thereby localizing the negative pressure near the negatively pressurized chamber. Microbubbles were removed within 5 mm of the negatively pressurized chamber; however, those in an area 7 mm and more from the chamber were not removed. Using the local removal method, a long-term perfusion test was performed, and no contact was confirmed between the bubbles and the simulated tissue for 72 h.

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“…Continued interest in designing and evaluating novel therapeutic drugs remains the utmost requirement, considering cancerrelated fatalities. [1] In general, the fatality rate due to lung cancer exceeds that of the colon, breast, and pancreatic cancer forms combined. [2] Despite significant efforts that have been put forward to curb the fatality rate, it still demands a novel design strategy in conjunction with the existing models, which may augment the efficacy of the drug.…”

Section: Introductionmentioning

confidence: 99%

“…Continued interest in designing and evaluating novel therapeutic drugs remains the utmost requirement, considering cancer‐related fatalities [1] . In general, the fatality rate due to lung cancer exceeds that of the colon, breast, and pancreatic cancer forms combined [2] .…”

Section: Introductionmentioning

confidence: 99%

Design, Synthesis, Biological Evaluation, and Molecular Docking Studies of N3‐Functionalized TZD‐Indole Conjugates as Potential Anticancer Agents

Ravi,

Gaddamedi,

Gopal Agrawal

et al. 2024

ChemistrySelect

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Investigation pertaining to the design and synthesis of therapeutic drugs against lung cancer remains in high demand, considering the high fatality rate associated with this disease. Herein, a carboxyl and ester functionalization at the N3 position of the TZD (thiazolidine‐2,4‐dione) moiety in an indole‐TZD conjugate was envisaged and evaluated as a potential anticancer drug. Although TIA (thiazoindole acid) did not demonstrate significant effectiveness against the A549 human lung adenocarcinoma cell line, the ester analog TIE (thiazoindole ester) showed high cytotoxic potential against the A549 cell line in their monolayer and spheroid form. Further, preliminary mechanistic investigations, such as cell viability studies and nuclear staining, revealed typical apoptotic features, including chromatin condensation, cellular shrinkage, and the formation of horseshoe‐shaped nuclei. In addition, the TIE compound can disrupt the F‐actin protein, thereby decreasing cell migration. Moreover, the cell cycle analysis revealed a notable halt in the G2/M phase. Further docking studies elucidate the interaction between TIE and the (peroxisome proliferator‐activated receptor) PPAR‐γ receptor. In summary, the findings of our investigation suggest that TIE compounds possess considerable efficacy as an anti‐proliferative agent and hold promise for therapeutic applications in lung cancer treatment.

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A novel design of microfluidic platform for metronomic combinatorial chemotherapy drug screening based on 3D tumor spheroid model

Cited by 9 publications

References 25 publications

Osteomatrix as a personalized 3D tissue-specific invasion test-bed for oral carcinoma

Osteomatrix as a personalized 3D tissue-specific invasion test-bed for oral carcinoma

Local Microbubble Removal in Polydimethylsiloxane Microchannel by Balancing Negative and Atmospheric Pressures

Design, Synthesis, Biological Evaluation, and Molecular Docking Studies of N3‐Functionalized TZD‐Indole Conjugates as Potential Anticancer Agents

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