A novel derivatization method for the determination of Fosfomycin in human plasma by liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometric detection via phase transfer catalyzed derivatization

Papakondyli, Theodora A.; Gremilogianni, Aikaterini M.; Megoulas, Nikolaos C.; Koupparis, Michael A.

doi:10.1016/j.chroma.2014.01.041

Cited by 14 publications

(4 citation statements)

References 24 publications

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“…7 Conversely, the fAUC/MIC ratio represents the best PK/PD targets against gram-negative pathogens at .83 and .40.8 for maximizing clinical efficacy in infections caused by Enterobacterales or Pseudomonas aeruginosa, respectively. 6,8 Fosfomycin has been measured in human tissues and bodily fluids using various techniques, including microbiological assays, 9 gas chromatography, 10,11 flow injection spectrophotometry, 12 ion-exchange chromatography, 13 high-performance liquid chromatographytandem mass spectrometry (LC-MS/MS), 14,15 and hydrophilic interaction liquid chromatography (HILIC) coupled with MS. 16,17 High concentrations of fosfomycin can be retrieved from human plasma, depending on the dose, delivery method, and drug elimination rate. 18 However, several measurement methods are affected by the limited sensitivity of analytical techniques.…”

Section: Introductionmentioning

confidence: 99%

“…Fosfomycin has been measured in human tissues and bodily fluids using various techniques, including microbiological assays, 9 gas chromatography, 10,11 flow injection spectrophotometry, 12 ion-exchange chromatography, 13 high-performance liquid chromatography – tandem mass spectrometry (LC-MS/MS), 14,15 and hydrophilic interaction liquid chromatography (HILIC) coupled with MS. 16,17…”

Section: Introductionmentioning

confidence: 99%

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Fast and Sensitive Analysis of Fosfomycin in Human Plasma Microsamples Using Liquid Chromatography–Tandem Mass Spectrometry for Therapeutic Drug Monitoring

Barone,

Conti,

Giorgi

et al. 2023

Therapeutic Drug Monitoring

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Background: Fosfomycin is an antibiotic recently repurposed as a potential combination treatment for difficult-to-treat Gram-negative bacterial infections. The pharmacokinetic features of fosfomycin have demonstrated that different pathophysiologic alterations may affect its exposure. Therapeutic drug monitoring may improve real-time management of fosfomycin therapy in different clinical scenarios. Objectives: To develop and validate a fast and sensitive liquid chromatography – tandem mass spectrometry method for measuring fosfomycin in human plasma microsamples (3 µL). Methods: Analysis was preceded by a user-friendly pre-analytical single-step process performed via a rapid chromatographic run of 2.5 minutes, followed by negative electrospray ionization and detection on a high-sensitivity triple quadrupole tandem mass spectrometer operated in the multiple reaction monitoring mode. European Medicines Agency guidelines were used to validate the specificity, sensitivity, linearity, precision, accuracy, matrix effects, extraction recovery, limits of quantification, and stability of the analytical method. Results: The new assay produced accurate (BIAS%: 0.9–9.1) and precise (coefficient of variation [CV]%: 8.1–9.5) measurements of fosfomycin over a concentration range of 1–1000 mg/L. Overall, analyte recovery was consistent (mean values: 91.2%–97.2%) at all tested concentration levels. The analyte was also stable in human plasma and the final extract under various storage conditions. The clinical applicability of the assay was confirmed through quantitation of plasma samples obtained from patients. Conclusions: A sensitive liquid chromatography – tandem mass spectrometry method for measuring fosfomycin in plasma was developed and validated according to the European Medicines Agency criteria. Quantitation of fosfomycin in clinical plasma samples confirmed that the assay is suitable for therapeutic drug monitoring in clinical scenarios.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fast and Sensitive Analysis of Fosfomycin in Human Plasma Microsamples Using Liquid Chromatography–Tandem Mass Spectrometry for Therapeutic Drug Monitoring

Barone,

Conti,

Giorgi

et al. 2023

Therapeutic Drug Monitoring

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show abstract

“…Derivatization is employed to improve the sensitivity of detection for these analytes. Most of the derivatization techniques used for ESI focus on introduction of a permanently charged moiety or readily ionizable group onto the analytes, whereas the derivatization techniques for API tend to introduce moieties with high proton affinity or electron affinity onto the analytes .…”

Section: Methods Of Detection and Quantification Of 5mc And Its Derivmentioning

confidence: 99%

Advances in detection and quantification of methylcytosine and its derivatives

Asenso

Wang

et al. 2019

J. Sep. Sci.

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Methylation of the fifth carbon atom in cytosine is an epigenetic modification of deoxyribonucleic acid that plays important roles in numerous cellular processes and disease pathogenesis. Three additional states of cytosine, that is, 5‐hydroxymethylcytosine, 5‐formylcytosine and 5‐carboxylcytosine, have been identified and associated with the diagnosis and/or prognosis of diseases. However, accurate measurement of those intermediates is a challenge since their global levels are relatively low. A number of innovative methods have been developed to detect and quantify these compounds in biological samples, such as blood, tissue and urine, etc. This review focuses on recent advancement in detection and quantification of four cytosine modifications, based on which, the development, diagnosis, and prognosis of diseases could be monitored through non‐invasive procedures.

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“…Recent data suggest high thermal stability of FOS powders and solutions as well long stability, even at room temperature, in biological samples using different approaches ranging from Thermogravimetry (TG) and Differential Scanning Calorimetry (DSC) to liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) or microbiological testing [ 25 , 26 , 27 , 28 , 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Stability Study of Fosfomycin in Elastomeric Pumps at 4 °C and 34 °C: Technical Bases for a Continuous Infusion Use for Outpatient Parenteral Antibiotic Therapy

Manca,

Palermiti,

Mula

et al. 2023

Pharmaceutics

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Background: Fosfomycin acts against aerobic Gram−/+ bacteria by blocking the synthesis of peptidoglycan. Its use has been currently re-evaluated for intravenous administration for the treatment of systemic infections by multidrug-resistant bacteria. Concentration-/time-dependent activity has been suggested, with potential clinical advantages from prolonged or continuous infusion. Nevertheless, little is known about Fosfomycin stability in elastomeric pumps. The aim of the present work was stability investigation before administration at 4 °C and during administration at 34 °C. Methods: InfectoFos® (InfectoPharm s.r.l., Milan, Italy) preparation for intravenous use in elastomeric pumps at 4 °C and 34 °C was analyzed following EMA guidelines for drug stability. Samples were analyzed with an ultra-high performance liquid chromatography coupled with tandem mass spectrometry method on a LX50® UHPLC system equipped with a QSight 220® (Perkin Elmer, Milan, Italy) tandem mass spectrometer. Results: Fosfomycin in elastomeric preparation is stable for at least 5 days at a storage temperature of 4 °C and 34 °C. Conclusions: The results suggest Fosfomycin eligibility for continuous infusion even in the context of outpatient parenteral antibiotic therapy. Therefore, this approach should be tested in clinical and pharmacokinetic studies, in order to evaluate the possible gains in the pharmacokinetic profile and the clinical effectiveness.

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A novel derivatization method for the determination of Fosfomycin in human plasma by liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometric detection via phase transfer catalyzed derivatization

Cited by 14 publications

References 24 publications

Fast and Sensitive Analysis of Fosfomycin in Human Plasma Microsamples Using Liquid Chromatography–Tandem Mass Spectrometry for Therapeutic Drug Monitoring

Fast and Sensitive Analysis of Fosfomycin in Human Plasma Microsamples Using Liquid Chromatography–Tandem Mass Spectrometry for Therapeutic Drug Monitoring

Advances in detection and quantification of methylcytosine and its derivatives

Stability Study of Fosfomycin in Elastomeric Pumps at 4 °C and 34 °C: Technical Bases for a Continuous Infusion Use for Outpatient Parenteral Antibiotic Therapy

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