A novel cysteine proteinase inhibitor from seeds of Enterolobium contortisiliquum and its effect on Callosobruchus maculatus larvae

Nunes, Natalia Neto dos Santos; Ferreira, Rodrigo S.; Sá, Leonardo Figueira Reis de; Oliveira, Antônia Elenir Amâncio; Oliva, Maria Luiza Vilela

doi:10.1016/j.bbrep.2020.100876

Cited by 3 publications

(4 citation statements)

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“…The functions of part‐specific genes reflect well‐known processes and pathways that are key to the physiology of each body part, such as fatty acid accumulation and response to maturation‐ and germination‐related phytohormones in seeds (abscisic acid and gibberellin, respectively) (Chauffour et al., 2019; Li et al., 2022; Ogawa et al., 2003), defense‐related genes in seed coats (Nunes et al., 2021; Shalovylo et al., 2021; Silva et al., 2018), nodulation‐related transporters and signaling components in root nodules (Banasiak et al., 2021; Niño‐González et al., 2019; Zeng et al., 2023), and photosynthesis‐ and plant growth‐related gene families in aerial parts (Jeena et al., 2019; Ren and Gray, 2015; Stortenbeker and Bemer, 2019). Collectively, our results validate the data reliability in the Soybean Expression Atlas v2.…”

Section: Resultsmentioning

confidence: 99%

The Soybean Expression Atlas v2: A comprehensive database of over 5000 RNA‐seq samples

Almeida‐Silva,

Pedrosa‐Silva,

Venancio

2023

The Plant Journal

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SUMMARYSoybean is a crucial crop worldwide, used as a source of food, feed, and industrial products due to its high protein and oil content. Previously, the rapid accumulation of soybean RNA‐seq data in public databases and the computational challenges of processing raw RNA‐seq data motivated us to develop the Soybean Expression Atlas, a gene expression database of over a thousand RNA‐seq samples. Over the past few years, our database has allowed researchers to explore the expression profiles of important gene families, discover genes associated with agronomic traits, and understand the transcriptional dynamics of cellular processes. Here, we present the Soybean Expression Atlas v2, an updated version of our database with a fourfold increase in the number of samples, featuring transcript‐ and gene‐level transcript abundance matrices for 5481 publicly available RNA‐seq samples. New features in our database include the availability of transcript‐level abundance estimates and equivalence classes to explore differential transcript usage, abundance estimates in bias‐corrected counts to increase the accuracy of differential gene expression analyses, a new web interface with improved data visualization and user experience, and a reproducible and scalable pipeline available as an R package. The Soybean Expression Atlas v2 is available at https://soyatlas.venanciogroup.uenf.br/, and it will accelerate soybean research, empowering researchers with high‐quality and easily accessible gene expression data.

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Section: Resultsmentioning

confidence: 99%

The Soybean Expression Atlas v2: A comprehensive database of over 5000 RNA‐seq samples

Almeida‐Silva,

Pedrosa‐Silva,

Venancio

2023

The Plant Journal

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“…Proteinase inhibitors may play a role as reserve proteins in plants ( Richardson, 1991 ; Hansen et al, 2007 ) and be involved in plant defense mechanisms. Cysteine PIs were previously isolated from seeds of the Enterolobium contortisiliquum tree targeting larval growth inhibition in the pest Collasobruchus maculatus ( Nunes et al, 2021 ). Several studies have shown feeding insects with transgenic plants that express proteinase inhibitors to delay insect growth and development, and to cause starvation and death ( Koiwa et al, 1998 ; Schuler et al, 1998 ; Mosolov et al, 2001 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of Proteases and Protease Inhibitors in Seeds of the Recalcitrant Forest Tree Species Quercus ilex

et al. 2022

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Proteases and protease inhibitors have been identified in the recalcitrant species Quercus ilex using in silico and wet methods, with focus on those present in seeds during germination. In silico analyses showed that the Q. ilex transcriptome database contained 2,240 and 97 transcripts annotated as proteases and protease inhibitors, respectively. They belonged to the different families according to MEROPS,1 being the serine and metallo ones the most represented. The data were compared with those previously reported for other Quercus species, including Q. suber, Q. lobata, and Q. robur. Changes in proteases and protease inhibitors alongside seed germination in cotyledon and embryo axis tissues were assessed using proteomics and in vitro and in gel activity assays. Shotgun (LC–MSMS) analysis of embryo axes and cotyledons in nonviable (NV), mature (T1) and germinated (T3) seeds allowed the identification of 177 proteases and 12 protease inhibitors, mostly represented by serine and metallo types. Total protease activity, as determined by in vitro assays using azocasein as substrate, was higher in cotyledons than in embryo axes. There were not differences in activity among cotyledon samples, while embryo axis peaked at germinated T4 stage. Gel assays revealed the presence of protease activities in at least 10 resolved bands, in the Mr range of 60–260 kDa, being some of them common to cotyledons and embryo axes in either nonviable, mature, and germinated seeds. Bands showing quantitative or qualitative changes upon germination were observed in embryo axes but not in cotyledons at Mr values of 60–140 kDa. Proteomics shotgun analysis of the 10 bands with protease activity supported the results obtained in the overall proteome analysis, with 227 proteases and 3 protease inhibitors identified mostly represented by the serine, cysteine, and metallo families. The combined use of shotgun proteomics and protease activity measurements allowed the identification of tissue-specific (e.g., cysteine protease inhibitors in embryo axes of mature acorns) and stage-specific proteins (e.g., those associated with mobilization of storage proteins accumulated in T3 stage). Those proteins showing differences between nonviable and viable seeds could be related to viability, and those variables between mature and germinated could be associated with the germination process. These differences are observed mostly in embryo axes but not in cotyledons. Among them, those implicated in mobilization of reserve proteins, such as the cathepsin H cysteine protease and Clp proteases, and also the large number of subunits of the CNS and 26S proteasome complex differentially identified in embryos of the several stages suggests that protein degradation via CNS/26S plays a major role early in germination. Conversely, aspartic proteases such as nepenthesins were exclusively identified in NV seeds, so their presence could be used as indicator of nonviability.

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“…The mean linear intercept (Lm) is the most-used parameter to demonstrate the presence of alveolar enlargement [17]. The reticulum with 50 lines (100 points) was coupled under an optical microscope (E200 MV, Nikon Corporation, Tokyo, Japan), and the analysis was performed by counting the intersections between the reticulum lines and the lung parenchyma.…”

Section: Lmmentioning

confidence: 99%

“…PI 0601390-2). Pep-3EcTI has eight amino acids in its composition, a molecular mass of 994.21 Da, and an isoelectric point of 9.75 [17].…”

Section: Introductionmentioning

confidence: 99%

Investigating the Effects of a New Peptide, Derived from the Enterolobium contortisiliquum Proteinase Inhibitor (EcTI), on Inflammation, Remodeling, and Oxidative Stress in an Experimental Mouse Model of Asthma–Chronic Obstructive Pulmonary Disease Overlap (ACO)

Barbosa,

da Silva,

João

et al. 2023

IJMS

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The synthesized peptide derived from Enterolobium contortisiliquum (pep3-EcTI) has been associated with potent anti-inflammatory and antioxidant effects, and it may be a potential new treatment for asthma–COPD overlap—ACO). Purpose: To investigate the primary sequence effects of pep3-EcTI in an experimental ACO. BALB/c mice were divided into eight groups: SAL (saline), OVA (ovalbumin), ELA (elastase), ACO (ovalbumin + elastase), ACO-pep3-EcTI (treated with inhibitor), ACO-DX (treated with dexamethasone), ACO-DX-pep3-EcTI (treated with dexamethasone and inhibitor), and SAL-pep3-EcTI (saline group treated with inhibitor). We evaluated the hyperresponsiveness to methacholine, exhaled nitric oxide, bronchoalveolar lavage fluid (BALF), mean linear intercept (Lm), inflammatory markers, tumor necrosis factor (TNF-α), interferon (IFN)), matrix metalloproteinases (MMPs), growth factor (TGF-β), collagen fibers, the oxidative stress marker inducible nitric oxide synthase (iNOS), transcription factors, and the signaling pathway NF-κB in the airways (AW) and alveolar septa (AS). Statistical analysis was conducted using one-way ANOVA and t-tests, significant when p < 0.05. ACO caused alterations in the airways and alveolar septa. Compared with SAL, ACO-pep3-EcTI reversed the changes in the percentage of resistance of the respiratory system (%Rrs), the elastance of the respiratory system (%Ers), tissue resistance (%Gtis), tissue elastance (%Htis), airway resistance (%Raw), Lm, exhaled nitric oxide (ENO), lymphocytes, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, TNF-α, INF-γ, MMP-12, transforming growth factor (TGF)-β, collagen fibers, and iNOS. ACO-DX reversed the changes in %Rrs, %Ers, %Gtis, %Htis, %Raw, total cells, eosinophils, neutrophils, lymphocytes, macrophages, IL-1β, IL-6, IL-10, IL-13, IL-17, TNF-α, INF-γ, MMP-12, TGF-β, collagen fibers, and iNOS. ACO-DX-pep3-EcTI reversed the changes, as was also observed for the pep3-EcTI and the ACO-DX-pep3-EcTI. Significance: The pep3-EcTI was revealed to be a promising strategy for the treatment of ACO, asthma, and COPD.

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A novel cysteine proteinase inhibitor from seeds of Enterolobium contortisiliquum and its effect on Callosobruchus maculatus larvae

Cited by 3 publications

References 63 publications

The Soybean Expression Atlas v2: A comprehensive database of over 5000 RNA‐seq samples

The Soybean Expression Atlas v2: A comprehensive database of over 5000 RNA‐seq samples

Identification of Proteases and Protease Inhibitors in Seeds of the Recalcitrant Forest Tree Species Quercus ilex

Investigating the Effects of a New Peptide, Derived from the Enterolobium contortisiliquum Proteinase Inhibitor (EcTI), on Inflammation, Remodeling, and Oxidative Stress in an Experimental Mouse Model of Asthma–Chronic Obstructive Pulmonary Disease Overlap (ACO)

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