A novel cysteine carbamoyl-switch is responsible for the inhibition of formamidase, a nitrilase superfamily member

Martínez‐Rodríguez, Sergio; Conejero-Muriel, Mayte; Gavira, José A.

doi:10.1016/j.abb.2018.12.008

Cited by 3 publications

(1 citation statement)

References 53 publications

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“…Compounds such as Hg 2+ ions, iodoacetamide, or iodoacetate inhibit the formamidases of B. cereus and H. pylori as they react with a catalytic cysteine residue (Skouloubris et al 2001 ; Soriano-Maldonado et al 2011 ). Urea and thiourea, which resemble formamide, inhibit the formamidases of B. cereus and M. methylotrophus (Wyborn et al 1996 ; Martínez-Rodríguez et al 2019 ), and the presence of Ni 2+ ions activates the H. pylori enzyme (Bury-Moné et al 2004 ).…”

Section: Formamidase: Phylogeny Enzyme Activity Biochemical and Genet...mentioning

confidence: 99%

Prospects of formamide as nitrogen source in biotechnological production processes

Schwardmann,

Benninghaus,

Lindner

et al. 2024

Appl Microbiol Biotechnol

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This review presents an analysis of formamide, focussing on its occurrence in nature, its functional roles, and its promising applications in the context of the bioeconomy. We discuss the utilization of formamide as an innovative nitrogen source achieved through metabolic engineering. These approaches underscore formamide’s potential in supporting growth and production in biotechnological processes. Furthermore, our review illuminates formamide’s role as a nitrogen source capable of safeguarding cultivation systems against contamination in non-sterile conditions. This attribute adds an extra layer of practicality to its application, rendering it an attractive candidate for sustainable and resilient industrial practices. Additionally, the article unveils the versatility of formamide as a potential carbon source that could be combined with formate or CO2 assimilation pathways. However, its attributes, i.e., enriched nitrogen content and comparatively limited energy content, led to conclude that formamide is more suitable as a co-substrate and that its use as a sole source of carbon for biomass and bio-production is limited. Through our exploration of formamide’s properties and its applications, this review underscores the significance of formamide as valuable resource for a large spectrum of industrial applications. Key points • Formidases enable access to formamide as source of nitrogen, carbon, and energy • The formamide/formamidase system supports non-sterile fermentation • The nitrogen source formamide supports production of nitrogenous compounds

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Section: Formamidase: Phylogeny Enzyme Activity Biochemical and Genet...mentioning

confidence: 99%

Prospects of formamide as nitrogen source in biotechnological production processes

Schwardmann,

Benninghaus,

Lindner

et al. 2024

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

The structures of the C146A variant of the amidase from Pyrococcus horikoshii bound to glutaramide and acetamide suggest the basis of amide recognition

Makumire

Weber

et al. 2022

Journal of Structural Biology

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Biotransformation of free cyanide to formic acid by a cyanide hydratase−formamidase cascade reaction

Martínková,

Kulik,

Rucká

et al. 2024

Process Biochemistry

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A novel cysteine carbamoyl-switch is responsible for the inhibition of formamidase, a nitrilase superfamily member

Cited by 3 publications

References 53 publications

Prospects of formamide as nitrogen source in biotechnological production processes

Prospects of formamide as nitrogen source in biotechnological production processes

The structures of the C146A variant of the amidase from Pyrococcus horikoshii bound to glutaramide and acetamide suggest the basis of amide recognition

Biotransformation of free cyanide to formic acid by a cyanide hydratase−formamidase cascade reaction

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