A novel CuFe2O4 nanospheres molecularly imprinted polymers modified electrochemical sensor for lysozyme determination

Liang, Axin; Tang, Bo; Hou, Huipeng; Sun, Liquan; Luo, Aiqin

doi:10.1016/j.jelechem.2019.113465

Cited by 34 publications

(11 citation statements)

References 48 publications

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“…An interesting application of MIP to the electrochemical detection of lysozyme has been recently proposed by Liang et al [ 71 ] who obtained good sensing performances but using a quite complex sensor assembly integrating CuFe 2 O 4 magnetic nanospheres. A very long-time procedure (about 70 h) is used for sensor development including preparation of nanoparticles and subsequent MIP chemical polymerization.…”

Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Polyscopoletin for the Electrochemical Detection of the Chronic Disease Marker Lysozyme

2020

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Herein we report the electropolymerization of a scopoletin based molecularly imprinted polymer (MIP) for the detection of lysozyme (Lyz), an enzymatic marker of several diseases in mammalian species. Two different approaches have been used for the imprinting of lysozyme based, respectively, on the use of a monomer-template mixture and on the covalent immobilization of the enzyme prior to polymer synthesis. In the latter case, a multi-step protocol has been exploited with preliminary functionalization of gold electrode with amino groups, via 4-aminothiophenol, followed by reaction with glutaraldehyde, to provide a suitable linker for lysozyme. Each step of surface electrode modification has been followed by cyclic voltammetry and electrochemical impedance spectroscopy, which has been also employed to test the electrochemical responses of the developed MIP. The sensors show good selectivity to Lyz and detect the enzyme at concentrations up to 292 mg/L (20 μM), but with different performances, depending on the used imprinting approach. An imprinting factor equal to 7.1 and 2.5 and a limit of detection of 0.9 mg/L (62 nM) and 2.1 mg/L (141 nM) have been estimated for MIPs prepared with and without enzyme immobilization, respectively. Competitive rebinding experiment results show that this sensing material is selective for Lyz determination. Tests were performed using synthetic saliva to evaluate the potential application of the sensors in real matrices for clinical purposes.

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Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Polyscopoletin for the Electrochemical Detection of the Chronic Disease Marker Lysozyme

2020

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“…Along with MIP NPs, other examples of the fruitful coupling of nanotechnology and imprinting technology have been illustrated in several works exploiting the integration of MIP film with both metallic [ 92 , 123 , 168 ] and carbon-based [ 55 , 88 , 169 , 170 ] nanomaterials in order to exploit their well-known properties in signal transduction, due to their increased surface area, remarkable conductivity, and excellent catalytic activity, all key elements in the design of an electrochemical sensor. The documented multifunctional properties of these nanomaterials, including high conductivity, interactive functions at the surface, and large surface-to-volume ratios, have indeed proved to increase sensing sensitivity [ 97 , 98 , 171 – 173 ].…”

Section: Mip Format Suitable For the Electrochemical Sensing Of Macro...mentioning

confidence: 99%

Electrochemical sensing of macromolecules based on molecularly imprinted polymers: challenges, successful strategies, and opportunities

2022

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Looking at the literature focused on molecularly imprinted polymers (MIPs) for protein, it soon becomes apparent that a remarkable increase in scientific interest and exploration of new applications has been recorded in the last several years, from 42 documents in 2011 to 128 just 10 years later, in 2021 (Scopus, December 2021). Such a rapid threefold increase in the number of works in this field is evidence that the imprinting of macromolecules no longer represents a distant dream of optimistic imprinters, as it was perceived until only a few years ago, but is rapidly becoming an ever more promising and reliable technology, due to the significant achievements in the field. The present critical review aims to summarize some of them, evidencing the aspects that have contributed to the success of the most widely used strategies in the field. At the same time, limitations and drawbacks of less frequently used approaches are critically discussed. Particular focus is given to the use of a MIP for protein in the assembly of electrochemical sensors. Sensor design indeed represents one of the most active application fields of imprinting technology, with electrochemical MIP sensors providing the broadest spectrum of protein analytes among the different sensor configurations. Graphical abstract

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“…Compared with traditional separation materials, an increasing number of researchers have applied magnetic materials containing excellent properties to extract trace substances in environmental, food, clinical, and bioanalytical areas. 32 The commonly used MNPs include Ni and its oxides, 33,34 CuFe 2 O 4 , 35,36 γ-Fe 2 O 3 37,38 and Fe 3 O 4 . 39,40 With the emergence of MIPs as an “artificial antibody”, MMIPs that combine the advantages of MNPs and MIPs, have entered the field of vision of scientific researchers.…”

Section: Preparation and Surface Modification Of Mmipsmentioning

confidence: 99%

Application progress of magnetic molecularly imprinted polymers chemical sensors in the detection of biomarkers

Wang

Yang

Pang

et al. 2022

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A novel CuFe2O4 nanospheres molecularly imprinted polymers modified electrochemical sensor for lysozyme determination

Cited by 34 publications

References 48 publications

Molecularly Imprinted Polyscopoletin for the Electrochemical Detection of the Chronic Disease Marker Lysozyme

Molecularly Imprinted Polyscopoletin for the Electrochemical Detection of the Chronic Disease Marker Lysozyme

Electrochemical sensing of macromolecules based on molecularly imprinted polymers: challenges, successful strategies, and opportunities

Application progress of magnetic molecularly imprinted polymers chemical sensors in the detection of biomarkers

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