A novel cucumisin-like serine protease from leaf of legume Canavalia ensiformis

Gonçalves, Rayane Natashe; Kalume, Dário Eluan; Ferrara, Maria Antonieta; Silva-López, Raquel Elisa da

doi:10.1007/s13562-020-00578-5

Cited by 4 publications

(1 citation statement)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, MS/MS scans were only triggered for precursor ions having a minimum signal threshold of 10,000 counts. Precursors selected for MS/MS scans were dynamically excluded for 30 s from a repeated product ion scan within a ± 10 ppm mass error (Gonçalves et al 2021).…”

Section: Crotalaria Spectabilis On Leishmania Amazonensismentioning

confidence: 99%

Antileishmanial effects of Crotalaria spectabilis Roth aqueous extracts on Leishmania amazonensis

PACHECO,

TEIXEIRA,

PASCHOAL

et al. 2023

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

Fifteen polar extracts from leaf, seed, pod, stem, flower and root of Crotalaria spectabilis were prepared using aqueous systems, based on the principles of green chemistry, and showed different protease inhibitor (PI) activities on trypsin, papain, pepsin and the extracellular L. amazonensis serine protease (LSPIII). The most pronounced inhibitory effect on LSPIII was observed in leaf (CS-P), root, stem, flower (CS-FPVPP) and pod (CS-VA) extracts. Crotalaria extracts exhibited low cytotoxicity on macrophages; however, they decreased the viability of L. amazonensis promastigotes and amastigotes, as observed in leaf (CS-AE, CS-P, CS-T and CS-PVPP), seed (CS-ST), flower and root (CS-RA) extracts. CS-P was chosen to study PI and secondary metabolites and a 10-12 kDa protein, analyzed by mass spectrometry, was identified as a serine PI homologous with papaya latex serine PI. Glycosylated flavonoids, such as quercetins, vitexin and tricin were the major secondary metabolites of CS-P. The presence of PIs in C. spectabilis is a new finding, especially in other organs than seeds since PIs have been reported only in seed legumes. Besides, this is the first report of antileishmanial activity of C. spectabilis extracts and the identification of serine polypeptide PI and glycosylated flavonoids from leaf.

show abstract

Section: Crotalaria Spectabilis On Leishmania Amazonensismentioning

confidence: 99%